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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
My fault or theirs?
    #5681986 - 05/27/06 10:57 PM (17 years, 8 months ago)

Pan Cyan Syringe.
From a vendor here.

Inoculated to:
1 Mea Agar Dish
2 Seperate Liquid Culutes of Seperate Batches
2 Grain Jars
2 PF Style Jars
1 h/poo, wbs, verm fp bag

Every medium got mold. Trich.
The mold came from the solution or needle in my opinion.
Maybe I'm wrong, here's where the mold was:

In the PF Jars the mold was under the small amount of mycelium.
Not near the top, at the injection sites.
In the petri dish it spread from the spore sites.
In the grain jar it spread just like the mycelium would have.
The liquid cultures got cloudy and had tiny suspended bits in them.

Is there anyway this is my fault, or was the solution bunk?
Every Pan Cyan inoculation was done in a closed glove box.
Still air in the room and in the box. Same as always when I use a glove box. Every other strain worked out well by the way.

Needle was even flame sterilized for a few of the above, but nothing, all mold.

I hate to bother vendors, but i think I got a bad one.

May I have some advice please??


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InvisibleCureCat
Strangest
 User Gallery

Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
Re: My fault or theirs? [Re: monstermitch]
    #5683014 - 05/28/06 10:16 AM (17 years, 8 months ago)

I'd say you're probably right.  It can happen, however rarely.  From the info provided, it seems not to have been your fault, but perhaps there is some consistent unsafe procedures you used among this syringe... Seems unlikely, as you said all the other strains have either colonized or begun growth without trich.
:undecided:  Dunno what else to say.


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OfflineMLBjammer
Invitro Freak
Male User Gallery

Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
Re: My fault or theirs? [Re: CureCat]
    #5683067 - 05/28/06 10:45 AM (17 years, 8 months ago)

Mitch, you got a bad one. Hey, it happens every now and then even with the best vendors. I am sure the vendor will replace your order, as you are a helpful poster and obviously a knowledgeable grower. As long as you are professional in your e-mail or PM, you should be good. Peace.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: My fault or theirs? [Re: MLBjammer]
    #5683180 - 05/28/06 11:37 AM (17 years, 8 months ago)

I did send a pm, but I'm not going to get a replacement.
I think I've isolated it and am currently growing it out on agar.
I only have one good dish that made it, so if this one stays good, than I'll be fine.

Thanks for your opinions so far...


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