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shroomydan
exshroomerite


Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Experimental Woodlover Tek
#5681243 - 05/27/06 06:47 PM (17 years, 8 months ago) |
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A few years ago I made a post about putting Gymnopilus spore water in a spray bottle and walking through the woods spraying dead logs. I don't know if that tek worked or not because I moved and haven't been back to check, but not long after I posted it, Tahoe made a post about putting Ps. cyan spores in a spray bottle with Karo and water. He let the LC colonize, and then sprayed freshly laid wood chips. He had some success, but he also had a good bit of contam problems due to left over sugars in the LC.
The other night I was thinking about the best way to get P. caerulipes from spores to wood chips, and I had this idea. Make a gallon or so honey water LC. Allow it to sit until visible myc growth stops (stirring occasionally of course). After the growth has stopped most of the sugar should be depleted.
Here's the new part: Pour the colonized LC through a fine mesh filter, rinse and resuspend in fresh water. Now you have LC with virtually no free sugar for contams to get a foot hold. Use this solution to soak fresh clean unpasteurized unsterilized wood chips. After a day or two, drain off the excess water. Theoretically, this should produce colonized wood chips in record time.
Any thoughts on this?
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5681260 - 05/27/06 06:52 PM (17 years, 8 months ago) |
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I also have a woodlovers LC underway without any sugars so I'm sure we'll be in touch now that I know your working on something similar.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5681271 - 05/27/06 06:54 PM (17 years, 8 months ago) |
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I take it you were wanting to directly spray down outdoor beds with the myceliated water? I'm wondering what would support the mycelium until it gets established into the wood? Perhaps you could have a bit of sterilized sawdust in the LC mix, that the mycelium would be attached to so it wouldn't pour off with the rest of the LC. That might sustain it until it began to colonize the wood. Good luck. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5681333 - 05/27/06 07:17 PM (17 years, 8 months ago) |
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Well I dont think this is exactly what your asking but since I have read this recently and remembered it.
The new Stamets book Mycelium Running, he talked about about spore oil solutions. Made from corn, canola, safflower oil, and that can be run in chain saws and such and sprayers.
Though it is only few paragraphs long.
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llamabox
Myco/Ethnoresearcher


Registered: 11/23/05
Posts: 564
Loc: Third moon of the Indole ...
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Re: Experimental Woodlover Tek [Re: RogerRabbit]
#5681339 - 05/27/06 07:19 PM (17 years, 8 months ago) |
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It may not be the same type of thing,but I have taken small amounts of myc rhizos and blended with distilled water. Then I dunked pasturised shredded cardboad in the water, in a spawn bag. Let sit overnight then drained water. It is now sitting in an incubation chamber.
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shroomydan
exshroomerite


Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Re: Experimental Woodlover Tek [Re: RogerRabbit]
#5681353 - 05/27/06 07:24 PM (17 years, 8 months ago) |
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I have observed that sterilization and even pasteurization of wood invites contamination, whereas fresh clean wood chips never contam during a myc transfer.
I do not want to spray outdoor beds. It is much easier to spawn them with colonized wood chips. This is an attempt to quickly colonize wood chips from spores for indoor fruiting.
The fresh dry wood chips could be submerged in the sugarless LC for a few days to hydrate them, and during that time the myc should stick to them.
After a few days the water could be drained off leaving hydrated wood chips covered in a thin layer of wood eating mycelium. I could be wrong, but I think the myc should have enough energy to start eating the wood.
The chips should be given lots of air and should be misted occasionally to keep them from drying out. There would be no need for filter patch bags or anything like that, because uncooked wood will not germinate spores, and is therefore extremely resistant to contamination.
Unlike the other wood lovers, P. caerulipes fruits at room temperature, and I think it will become a very important species once an easy spore-to-fruit tek is written. If this works, it could be done without agar and without a pressure cooker.
I guess the sticking point is whether the washed myc will be able to colonize the wood. Time for an experiment?
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MagicalKnife
Shroomologist


Registered: 02/12/06
Posts: 135
Last seen: 15 years, 5 months
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5681367 - 05/27/06 07:33 PM (17 years, 8 months ago) |
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How easily do sterilized woodchips contaminate? I always thought being made from wood they couldn't be host to as many contaminants as a PF cake/WBS.
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5681372 - 05/27/06 07:34 PM (17 years, 8 months ago) |
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Well that does sound very cool and you sounds like you got a good game plan, I wish I new more to help out.
But since that is not the case I will find this interesting to check out.
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shroomydan
exshroomerite


Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Re: Experimental Woodlover Tek [Re: MagicalKnife]
#5681395 - 05/27/06 07:45 PM (17 years, 8 months ago) |
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I found out the hard way that sterilized sawdust will support red and green mold as well as cobweb. I think cooking breaks down some of the cellulose into glucose, allowing the comtam to get a foothold.
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Morelman
LC Master


Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Experimental Woodlover Tek *DELETED* [Re: shroomydan]
#5681667 - 05/27/06 09:09 PM (17 years, 8 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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kilroy
Hightimes



Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit
Last seen: 13 years, 3 months
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Re: Experimental Woodlover Tek [Re: Morelman]
#5681995 - 05/27/06 11:00 PM (17 years, 8 months ago) |
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I too am going to give this a try and like what you have for an idea. We should all keep in contact so we can get this to work. I think it would be pretty neat to get this one going and it also gives me new things to try with wood loveing spores. Wish you (us) all good luck in triing to bring in a new one to play with.
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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Pinhead
Oregano


Registered: 05/13/00
Posts: 1,819
Loc: Hootersville
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5683036 - 05/28/06 10:28 AM (17 years, 8 months ago) |
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Wow!! Right before reading this post, I started my own LC of P. caerulipes. My plan was to just get a squirt bottle and spray down an area that gets flooded yearly around a creek. Maybe now I'll re plan my strategy a little and rinse my LC and maybe even soak some hardwood chips before spreading.
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Psychoslut
The Mother Fucking Bear-o-dactyl

Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: Experimental Woodlover Tek [Re: shroomydan]
#5683046 - 05/28/06 10:35 AM (17 years, 8 months ago) |
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Is there a reason you cant or don't want to make grain spawn?
IMO LC just isn't established enough to do very well to directly inoculate wood chips but Ive never tried I'm just assuming.
Stamets has a recipe for reishi wood chips where he soaks the chips in water with some molasses in there. But he sterilizes it and spawns it with grain in front of a hepa. Just food for thought.
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[quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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Morelman
LC Master


Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Experimental Woodlover Tek *DELETED* [Re: RogerRabbit]
#5721165 - 06/06/06 11:05 PM (17 years, 7 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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Morelman
LC Master


Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Experimental Woodlover Tek *DELETED* [Re: Morelman]
#5721268 - 06/06/06 11:28 PM (17 years, 7 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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kilroy
Hightimes



Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit
Last seen: 13 years, 3 months
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Re: Experimental Woodlover Tek [Re: Morelman]
#5721283 - 06/06/06 11:32 PM (17 years, 7 months ago) |
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Pellet stove fuel, what is this and where are they found plz.
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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Morelman
LC Master


Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Experimental Woodlover Tek *DELETED* [Re: kilroy]
#5721296 - 06/06/06 11:38 PM (17 years, 7 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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kilroy
Hightimes



Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit
Last seen: 13 years, 3 months
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Re: Experimental Woodlover Tek [Re: Morelman]
#5721325 - 06/06/06 11:51 PM (17 years, 7 months ago) |
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ty
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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