Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemnmn
Stranger
Male

Registered: 05/27/06
Posts: 3
Loc: Canada
Last seen: 7 years, 1 month
first time attempt at pf-tek... contamination?
    #5681228 - 05/27/06 06:37 PM (17 years, 8 months ago)

i am trying cultivation for the first time with the pf-tek and the b+ strain. i inoculated 12 jars about 1 week ago and have not seen any kind of mycelial growth in any of them. near the inoculation sites though, i have noticed what looks like a slightly yellow discolouration of the substrate. in some jars it is more widespread than in others. even in one jar which was accidentally not properly sterilized, no other contaminants are present. but no less, out of 12 jars, not one has anything in it that looks remotely like mycelium.

my first guess is that there is not enough moisture in the jars either as a result of too high heat in sterilization or just not enough water added to the substrate initially.

i have more spores in other strains - should i try starting over? any other advice is appreciated as well.


Extras: Filter Print Post Top
OfflineSnaggletooth
Stranger in a Strange Land
Male User Gallery

Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
Re: first time attempt at pf-tek... contamination? [Re: mnmn]
    #5681261 - 05/27/06 06:52 PM (17 years, 8 months ago)

Well you would now better if you prepared the jars correctly.


But a week is not unheard of, so I would wait it out for now.


--------------------


Atheist Chat


Extras: Filter Print Post Top
InvisibleEasy_Cheese
Cheese in a can

Registered: 05/12/06
Posts: 62
Re: first time attempt at pf-tek... contamination? [Re: mnmn]
    #5681417 - 05/27/06 07:55 PM (17 years, 8 months ago)

what temperture are they incubating at?Ive grown jars at room temp(low-mid 60s)that took 7-10 days to show signs of germination before.
As far as the fact that you see yellow spots at the injection points...well that sounds like a contam but hard to say without seeing it.If you have 1 jar that you admit wasnt steralized properly is it possable they all suffered that fate?

And a good rule of thumb to avoid innoculating too soon is to just leave the jars right in the PC overnight.That way they are in a sterile enviroment while cooling and by the next morning they arre ready to go.


--------------------
....Said the can of Cheese!


Extras: Filter Print Post Top
Offlinemnmn
Stranger
Male

Registered: 05/27/06
Posts: 3
Loc: Canada
Last seen: 7 years, 1 month
Re: first time attempt at pf-tek... contamination? [Re: mnmn]
    #5681510 - 05/27/06 08:25 PM (17 years, 8 months ago)

they're at a pretty even 80-82 degrees.

what i observed is not so much yellow spots as it is yellowing on the vermiculite. it's very faint and not opaque - doesn't seem to have a 'body' of its own. i thought that it might be bacteria.

as for the questionably sterilized jar - it just happened to be sterilized inconsistently with the rest of the jars. though tried to compensate, i can't be -sure- that was as thorough as the rest.


Extras: Filter Print Post Top
OfflineMLBjammer
Invitro Freak
Male User Gallery

Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
Re: first time attempt at pf-tek... contamination? [Re: mnmn]
    #5682779 - 05/28/06 08:19 AM (17 years, 8 months ago)

If you don't see germination of any kind in 2 weeks, I would advise you to start over. The yellowing does not sound good.


Extras: Filter Print Post Top
InvisibleAtheist
Stranger
Male User Gallery

Registered: 01/24/06
Posts: 13,705
Loc: USA
Re: first time attempt at pf-tek... contamination? [Re: MLBjammer]
    #5682791 - 05/28/06 08:24 AM (17 years, 8 months ago)

ya 2 weeks is a long ass time, i dont think anything is going to happen


Extras: Filter Print Post Top
Offlinekilroy
Hightimes
Male

Folding@home Statistics
Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit Flag
Last seen: 13 years, 3 months
Re: first time attempt at pf-tek... contamination? [Re: Atheist]
    #5684449 - 05/28/06 07:57 PM (17 years, 8 months ago)

I will let you know from some of my mistake that I have made with my first grow. I had to add a little more water then what is put in the pf-tek recipe, do the thing of tilting the bowl to see if you just see a little puddle of water forming and be sure to mix vermiculit and water first then brf, next make sure that you innoc through the dry vermiculite. Put the needle in at an angle towards the side of the jar and see it go down the side of the jar, my first time I just squirted it on top of the dry vermiculit(lol). I hope this helps, just in case you made the same mistakes I did, and one more very immportant peice of advice that everyone pounded me with(lol) patiences is very very big with this type of growing. GL


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* PF-Tek or Rye grain DrD525 10,997 17 06/03/16 01:57 PM
by openmindedcanvas
* Re: Useful observation concerning the PF Tek Anonymous 1,547 7 08/03/00 09:54 AM
by Anonymous
* Mazatapec in standard PF Tek jars? Transender46 3,559 4 04/24/02 10:07 PM
by Egyn
* PF Tek Yield? AcidMan42069 26,026 7 05/06/09 09:45 PM
by Doc_T
* Newbie on PF Tek
( 1 2 all )
hazeblaze 6,257 26 04/11/17 08:55 PM
by tanoak
* *deleted* a3d0a3m 1,643 3 10/12/01 11:14 AM
by BeppoMarx
* PF Tek....casing Question The_Doc 1,329 2 05/11/02 06:48 AM
by StainBlue
* Re: Anybody have experience with PF tek? Anonymous 5,282 6 12/19/99 01:25 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,247 topic views. 19 members, 197 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.