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Offlineaccesstwo
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clone fruitbody on popcorn
    #5679978 - 05/27/06 10:14 AM (17 years, 8 months ago)

can you clone the inside of a fruit body on popcorn?


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InvisibleFooMan
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5679990 - 05/27/06 10:17 AM (17 years, 8 months ago)

That's an interesting question. You always see clones on agar. Why not just skip the agar and go straight to grains? I'm sure it's been done, I'm just wondering why you don't see or hear about it it much.


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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: FooMan]
    #5680019 - 05/27/06 10:30 AM (17 years, 8 months ago)

im going to try it with one of my fruitbodies sometime nextweek


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InvisiblePsychoslut
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680054 - 05/27/06 10:39 AM (17 years, 8 months ago)

theres a ghetto tek somwhere for brown rice agar. All it is is moist brown rice flour in the bottom of a half pint.


http://www.shroomery.org/index.php/par/25948


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: Psychoslut]
    #5680179 - 05/27/06 11:21 AM (17 years, 8 months ago)

might try that.

do you know about popcorn?


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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680187 - 05/27/06 11:25 AM (17 years, 8 months ago)

thanks for the great link. i think im going to clone the next largest fruit body of my second flush-i tried an lc but i don't know if its working?

came store bought brf work as well?


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OfflineRogerRabbitM
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680209 - 05/27/06 11:35 AM (17 years, 8 months ago)

Clones have been successfully transferred to grains. The reason agar is usually used is because it's really hard to get a clone contaminant free. Agar lets you transfer actual mycelium away from contaminants. You'll likely go through a lot of grain jars, but if you do enough, one of them will work. Young, rapidly growing fruits from the first flush are best suited for cloning.
RR


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InvisiblemushboyMDiscord
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680266 - 05/27/06 12:01 PM (17 years, 8 months ago)

I tried that tek with the brf paste. didnt work and got dried out really fast.

two days ago i did some grain - poo, one quart is about 30% in 2days. Im transfering about 20 half pints of brf to quarts of poo.

also, you can clone a shroom on cardboard. just use a small amount of tissue and pit on a piece of cardboard. ripped it down the middle to form 2 halfs of cardboard. soak in water with tiny bit of h2o2. put in glovebox or something similar, than transfer that colonzied piece to spawn/subsrtate of your choice.


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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: mushboy]
    #5680312 - 05/27/06 12:26 PM (17 years, 8 months ago)

maybe you should have made smaller holes in the lid and tried again

i think i will try the brf paste, with a very(small nail) small hole with tyvek


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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680316 - 05/27/06 12:28 PM (17 years, 8 months ago)

isn't agar suppossed to be keep wrapped so it doesn't dry out?
i think i will try 3, two wrapped and one open


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OfflineRogerRabbitM
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680329 - 05/27/06 12:33 PM (17 years, 8 months ago)

Agar is wrapped with parafilm to prevent contamination, not to prevent drying. You won't be growing on agar for more than a week or two anyway, so drying out isn't a problem.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineaccesstwo
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Re: clone fruitbody on popcorn [Re: RogerRabbit]
    #5680342 - 05/27/06 12:40 PM (17 years, 8 months ago)

oh

so basically when using this tek i should only use a small hole


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InvisiblePsychoslut
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Re: clone fruitbody on popcorn [Re: accesstwo]
    #5680481 - 05/27/06 01:37 PM (17 years, 8 months ago)

There is a tek in one of stamets books for getting clean clones and prints. Basically you just sterilize a inch or two of grains in the bottom of a 2 quart jar, inoculate it, let it colonize and let it fruit in the jar. That way the jar can be taken into a glove box and the mushroom specimen stays pretty much contaminant free.

Popcorn makes good spawn it just needs to be simmered about twice as long as wbs.

IMO newbs would most likely have a better chance of getting moisture content right on popcorn than wbs because its easier to drain and all that.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineaccesstwo
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Registered: 04/29/06
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Last seen: 12 years, 5 months
Re: clone fruitbody on popcorn [Re: Psychoslut]
    #5680578 - 05/27/06 02:15 PM (17 years, 8 months ago)

im going to try the "fahlstar popcorn isolation/myc syringe" first, i already made two popcorn jars(filled 1/5 full)

im also going to try two brf agar jars

so in total i should have 4 substrains to choose from. if the brf don't work i will have 2 substrains to work with.


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