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Offlinetrentallica
Lord Trentnar
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Registered: 05/01/06
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can i use boiling water to sterilize???
    #5678045 - 05/26/06 07:07 PM (17 years, 8 months ago)

boiling water wont kill endospores. i understand that. i don't have a PC just yet and i want to use WBS. my question is this. with prepping the WBS it is soaked overnight to hatch any endospores making them susceptible to the heat of boiling water. at that point can i then get satisfactory results sterilizing with just boiling water??
this is kinda of inline with the " fractional sterlization" method.


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OfflineSnaggletooth
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Re: can i use boiling water to sterilize??? [Re: trentallica]
    #5678053 - 05/26/06 07:12 PM (17 years, 8 months ago)

No, kinda fractional sterilization in not fractional sterilization.


Though if you wanted to try, WBS is cheap enough to give it a go.


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OfflineALHOFF177A17
DoWn RU?

Registered: 01/23/02
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Re: can i use boiling water to sterilize??? [Re: trentallica]
    #5678146 - 05/26/06 07:41 PM (17 years, 8 months ago)

When I used to do WBS I had a few jars the contammed after a 45 min PC run. One even had a rhizomorphic fungus sprouting from a single seed.


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Offlinetrentallica
Lord Trentnar
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Re: can i use boiling water to sterilize??? [Re: ALHOFF177A17]
    #5678241 - 05/26/06 08:10 PM (17 years, 8 months ago)

so should i go full fractional sterilization and boil for 30 min 3 days in a row??


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OfflineALHOFF177A17
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Re: can i use boiling water to sterilize??? [Re: trentallica]
    #5678322 - 05/26/06 08:33 PM (17 years, 8 months ago)

Well why not. worst that will happen is you get a cake of mold. Try it and tell all if it works.


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Offlinetrentallica
Lord Trentnar
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Registered: 05/01/06
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Re: can i use boiling water to sterilize??? [Re: ALHOFF177A17]
    #5678423 - 05/26/06 09:03 PM (17 years, 8 months ago)

Quote:

ALHOFF177A17 said:
Well why not. worst that will happen is you get a cake of mold. Try it and tell all if it works.




call me on it


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