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Offlinethedesolateone
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Registered: 05/10/06
Posts: 5
Last seen: 17 years, 4 months
Would appriciate a second opinion(s) on my current situation...
    #5675003 - 05/25/06 10:31 PM (17 years, 8 months ago)

Well let me explain the current situation to you all. I knocked up 12 PF Style jars a week ago, 6 B+ strain and 6 Cambodian, I just checked them today and the B+ jars are moving along rather nicely but I noticed that the 6 Cambodian are developing questionable greenish (trich?) spots. Now with the background information aside here's my question: is there a possibility that my Cambodian syringe could have been compromised? I mean seeing as how all 12 jars were filled with the same substrate batch, pressure cooked all at the same time, inoculated all at the same time in the same (what i would believe to be a pretty sterile environment-I showered, wiped all surfaces in bleach followed by 91% rubbing alcohol, dressed in clean clothes, wore a hat and a filtration mask, sprayed Lysol on everything including myself, sprayed oust profusely, ran a air purifier/ionizer for about a half an hour before inoculation in the bathroom), covered all the inoculation holes with micro-pore tape right after inoculation and incubated all in the same chamber. The only difference I can find between the preparation of the two jars is in the strains I used  :confused:. Any response is greatly appreciated. BTW the syringes were from a reputable vendor who I won't name here out of respect so don't ask.


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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
Re: Would appriciate a second opinion(s) on my current situation... [Re: thedesolateone]
    #5676069 - 05/26/06 06:20 AM (17 years, 8 months ago)

Yes, occasionally a vendor will have contammed syringes. If they are a shroomery vendor you can go to the vendor forum and find out who they're rep is and PM them.


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Offlinethedesolateone
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Registered: 05/10/06
Posts: 5
Last seen: 17 years, 4 months
Re: Would appriciate a second opinion(s) on my current situation... [Re: thedesolateone]
    #5676167 - 05/26/06 07:37 AM (17 years, 8 months ago)

Alright well I'll try that and see what happens...thanks alot for your reply


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InvisibleCureCat
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Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
Re: Would appriciate a second opinion(s) on my current situation... [Re: thedesolateone]
    #5676806 - 05/26/06 11:40 AM (17 years, 8 months ago)

Yes, if it seems that is the only difference and ALL 6 became contammed, then I think it's gotta be the syringe or even solution itself that was the source of contamination.  Your sterile methods sound thorough  :smile:


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Offlinethedesolateone
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Registered: 05/10/06
Posts: 5
Last seen: 17 years, 4 months
Re: Would appriciate a second opinion(s) on my current situation... [Re: thedesolateone]
    #5677372 - 05/26/06 02:42 PM (17 years, 8 months ago)

Glad to see someone sees my reasoning could possibly be correct, thanks for the complement too, I read and read and read some more until finally I decided to formulate my own sterilization methods built from the best of what I read. It seems to have worked for the 6 B+'s so I guess my pouring over of information paid off IMHO...


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