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OfflineRogerRabbitM
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Re: Agar Failure :( [Re: Oatman2000]
    #5678184 - 05/26/06 07:52 PM (17 years, 8 months ago)

For cloning, I don't use anything but MEA. (Malt Extract Agar). A fresh clone should be fuzzy within 48 hours, and spreading mycelium by the third day. Of course, it should go without saying that cloning a young, rapidly growing mushroom from the first flush is far preferable to cloning a large, old, spored out grandpa from the third or fourth flush.
RR


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InvisibleFooMan
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Re: Agar Failure :( [Re: RogerRabbit]
    #5678197 - 05/26/06 07:56 PM (17 years, 8 months ago)

Quote:

RogerRabbit said:
For cloning, I don't use anything but MEA. (Malt Extract Agar). A fresh clone should be fuzzy within 48 hours, and spreading mycelium by the third day.




Is there any particular benefit to using MEA? I've always used PDA and haven't had much luck cloning.


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OfflineRogerRabbitM
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Re: Agar Failure :( [Re: FooMan]
    #5678222 - 05/26/06 08:02 PM (17 years, 8 months ago)

Mushroom mycelium just seems to like it. Sometimes I put a teaspoon of powdered activated carbon into each liter of MEA, especially if I'm germinating spores or cloning. I skip the charcoal for transfers once the mycelium is growing. Lots of guys use potato agar, but personally I found it to be ok, but not great. I think a sponsor or two sell pre-mixed MEA powder. Just add water and pc.
RR


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InvisibleFooMan
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Re: Agar Failure :( [Re: RogerRabbit]
    #5678313 - 05/26/06 08:31 PM (17 years, 8 months ago)

I'll give it a shot.


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