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OfflineFantazee
journeyman
Registered: 11/24/01
Posts: 94
Loc: New England
Last seen: 16 years, 7 months
Agar confuses me...
    #5674297 - 05/25/06 07:17 PM (17 years, 8 months ago)

The more I read about Agar, the more confused I get...My biggest question is what to do with it once it is growing mycelium...I've read all sorts of information...Some say you use sections of mycelium to inoculate seed jars...Some say you use slices of agar to do the same...Some suggest making a mycelium water solution and using that to inoculate large amounts of sterilized straw...So I ask you...

What's the best use for Agar???


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InvisibleMrMaddHatter
Dementia praecox
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Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
Re: Agar confuses me... [Re: Fantazee]
    #5674367 - 05/25/06 07:38 PM (17 years, 8 months ago)

Depends. Once you've successfully cultured out what you're looking for, you can go any direction with it.

When I get a good culture grown out on agar, I'll usually use a piece to inoculate a grain jar, then another piece to inoculate a liquid solution, and whatever pieces left over to inoculate some more dishes.


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Offlinesoulsizzle
nobody f**kswith The Jesus

Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
Re: Agar confuses me... [Re: MrMaddHatter]
    #5674831 - 05/25/06 09:51 PM (17 years, 8 months ago)

For a moment, I though you were saying that Agar - the person who posts on this message board on a regular basis - confuses you :grin:


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Agar confuses me... [Re: soulsizzle]
    #5674939 - 05/25/06 10:18 PM (17 years, 8 months ago)

Agar is for germinating spores and isolating strains from each other and away from contaminants. You can use agar wedges to inoculate jars, which you then use for grain to grain transfers to expand the mycelium. Agar itself isn't used to expand mycelium.
RR


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