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Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures, Substrate Mix   North Spore Bulk Substrate, Injection Grain Bag

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Offlinejohndoeradio
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Newbie Question?
    #5673650 - 05/25/06 04:17 PM (17 years, 8 months ago)

Sterilized my one pint jars last night using the pf-tek, and boiling/steam method. I just inoculated the jars and while doing so noticed dull colored "residue", maybe just moisture, on the side and bottom of some of the jars. It Is this a problem?

Any help would be awesome. Thanks guys.


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Offlinemakaveli8x8
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Re: Newbie Question? [Re: johndoeradio]
    #5673661 - 05/25/06 04:20 PM (17 years, 8 months ago)

as long as its steril and your water content is right you should be fine. i mean you just sterilized it i highly doubt its contamed already if thats what your worried about.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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Offlinejohndoeradio
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Re: Newbie Question? [Re: makaveli8x8]
    #5673671 - 05/25/06 04:23 PM (17 years, 8 months ago)

and if thers is a dry verm layer on top, with foil and no tape covering innoc holes, should i not worry about any cover and just lose the foil top.

Once again you all are awesome.


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Offlinemakaveli8x8
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Re: Newbie Question? [Re: johndoeradio]
    #5673676 - 05/25/06 04:25 PM (17 years, 8 months ago)

no you want that foil there man, that verm is your second line of defence. You really should have put a wad of pollyfill on the top under that foil and screwed your lid over it all.

but you'll prob be ok just keep foil over it.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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Invisiblemonstermitch
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Re: Newbie Question? [Re: makaveli8x8]
    #5673707 - 05/25/06 04:36 PM (17 years, 8 months ago)

Quote:

makaveli8x8 said:
no you want that foil there man, that verm is your second line of defence. You really should have put a wad of pollyfill on the top under that foil and screwed your lid over it all.

but you'll prob be ok just keep foil over it.




I disagree.

If you have dry verm on top, do not use tape and do not use foil.

that foil is trapping moisture and gas exchange keeping it damp underneath the foil. This will promote contams quite a bit.

foil is only to be used to keep water out of the jars during sterilization. After sterilization it is to be prompty removed.

that is my opinion.


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Offlinezeegos
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Re: Newbie Question? [Re: monstermitch]
    #5673737 - 05/25/06 04:45 PM (17 years, 8 months ago)

Does that mean that jar lids can be removed?


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Offlinejohndoeradio
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Re: Newbie Question? [Re: monstermitch]
    #5673751 - 05/25/06 04:47 PM (17 years, 8 months ago)

im gonna take a few off and leave a few on. Maybe we can get a few more comments on foil suggestions. Next time i will use a tyvekk filter.

As far as water content i just poured filtered tap water into a bowl with verm till it was pretty moist, then mixed in brf, and a little bone meal in a few. They are now in a TIT incubater with a little tupper of perlite and water at 80f and about 85 humid. I will try not to take off the top, but i need to check temp and humid once a day right?


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Invisiblemonstermitch
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Re: Newbie Question? [Re: zeegos]
    #5673753 - 05/25/06 04:47 PM (17 years, 8 months ago)

on a pf jar with dry verm...

yeah, it does. You only want the gas exchange of the 4 holes though.

If you remove the lid, it will dry out the substrate.


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Offlinemakaveli8x8
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Re: Newbie Question? [Re: johndoeradio]
    #5673775 - 05/25/06 04:56 PM (17 years, 8 months ago)

Quote:

johndoeradio said:
im gonna take a few off and leave a few on. Maybe we can get a few more comments on foil suggestions. Next time i will use a tyvekk filter.

As far as water content i just poured filtered tap water into a bowl with verm till it was pretty moist, then mixed in brf, and a little bone meal in a few. They are now in a TIT incubater with a little tupper of perlite and water at 80f and about 85 humid. I will try not to take off the top, but i need to check temp and humid once a day right?




um wait a min...should his jars be in a tupper with perlite and water in the bottom??? or are you just doing that because you have a heater under there?


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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Invisiblemonstermitch
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Re: Newbie Question? [Re: makaveli8x8]
    #5673792 - 05/25/06 05:02 PM (17 years, 8 months ago)

If you have a pf style jar with a layer of dry vermiculite on the top:

Do not use foil
Do not use tyvek
Do not use micropore
Do not use poly-fil
Do not use filter discs
Do not use anything except for the verm, lid, and band.

When you start messing with what works, it stops working.


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Invisiblemonstermitch
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Re: Newbie Question? [Re: monstermitch]
    #5673796 - 05/25/06 05:04 PM (17 years, 8 months ago)

p.s. an incubator should not be humid.

no need at all for perlite in an incubator.
no need to monitor humidity in an incubator.


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Offlinejohndoeradio
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Re: Newbie Question? [Re: monstermitch]
    #5673842 - 05/25/06 05:15 PM (17 years, 8 months ago)

My jars dont have perlite in them, just a seperate small tupper with like a 1/4 in of perlite and just a little less water. the jars are sitting next to the tupper. My TIT is water in bottom tub with a heater.

Im about to take off all the foil.


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Offlinejohndoeradio
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Re: Newbie Question? [Re: johndoeradio]
    #5673851 - 05/25/06 05:17 PM (17 years, 8 months ago)

ok so ill take out the perlite also.

and i shouldnt let the top come off too much right. New air brings new contams right?


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Offlinemakaveli8x8
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Re: Newbie Question? [Re: johndoeradio]
    #5673876 - 05/25/06 05:22 PM (17 years, 8 months ago)

you want your lid with holes on the jar, with the screw lid part all the way on as well.

you want these jars sitting somewhere warm. and sealed from external enviroment. IN tupperwear.

but you don't need water or perlite in the bottom because it serves no use at this point. the jars just need to be warm...its humid enough inside the jars.

when you birth the cakes...(take them out of the jars) you put them somewhere humid...perlite and water....but you wouldn't need heat anymore...but you would need light and more air exchanges.

hope this helps.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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Offlinejohndoeradio
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Re: Newbie Question? [Re: makaveli8x8]
    #5674001 - 05/25/06 05:51 PM (17 years, 8 months ago)

im in a place where indoor humidity is less than 30percent. I d like to keep it higher than that inside my tubs. its a pain in the ass to keep temps up, a water heater can be trying on patience. (ie) lifting the tub after its been secured down into the water is the biggest pain.

If you do incubate in open air, are you pretty much s.o.l., or will the verm layer protect. my room temp is 83+ and keepping the water heater consistant is hard. so therefore it only like 76-80 in the incubator if the water isnt warm. my room can heat better.


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Invisiblemonstermitch
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Re: Newbie Question? [Re: johndoeradio]
    #5674013 - 05/25/06 05:55 PM (17 years, 8 months ago)

your tit can be opened at will.
the only thing to worry about there is temp dropping,
not air exchange.
That dry layer of vermiculite will protect your jars, it really will.

I incubate most all of my jars in open air.
If I were you, I would incubate in a box in your room.
No tit.
83 is great, perfect for me.
my incubating room goes from 80-83.

you are set.


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Offlinejohndoeradio
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Re: Newbie Question? [Re: monstermitch]
    #5674087 - 05/25/06 06:15 PM (17 years, 8 months ago)

thanks.

(btw used a 10cc syringe on six one pint jars.)

Ill be using casing after they are done. The thing is i didnt use the right jars. so its gonna be hard to get em out. Since im using a casing method can i just break up the myc inside the jar then dump. If not what should i do?



Edited by johndoeradio (05/25/06 06:17 PM)


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Offlinejohndoeradio
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Re: Newbie Question? [Re: johndoeradio]
    #5674893 - 05/25/06 10:06 PM (17 years, 8 months ago)

anyone with anything else to add?


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Offlinemakaveli8x8
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Re: Newbie Question? [Re: johndoeradio]
    #5675498 - 05/26/06 12:47 AM (17 years, 8 months ago)

yeah ive never done casing but from what ive read your sopposed to break the cakes up...so it doesn't matter where you do it...gl and post up some pics if you get a chance, or at least an update.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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