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Invisibleohmatic
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mex-a metabolic waste - excreted liquid experiment
    #5667877 - 05/24/06 08:37 AM (17 years, 11 months ago)

hey !

im currently experimenting with several mex a isolates which i started out as a mutlispore inoculation on pda.

about half a year later i took the plate from the fridge and for my surprise it had formed various sclerotia already
so i started to tranfer them to individual dishes in order to isolate.

the isolates are now 24 days old and are already producing new sclerotia.

masterplate:


ok so i started various isolates, and two of them particularely caught my interest.

they somehow formed blobs of some waterish looking liquid on top of the sclerotia
forming on the agar which looks absolutely stunning, and i wonder what it could be ?

   

has anybody got a clue what that excreted liquid may be ?



update
as i made transfers of the various isolates yesterday, also examined the blobs on the culture in front of the hood.

it seemed as if they had a sort of coating, and i think those were going to develop
into sclerotia hmm ..

some pics for the porn lovers :wink:



im baffled by teh sclerotia lol, guess this one is a keeper :smirk:

ah jeah i punctured one of the blobs and wiped the liquid onto a agar plate.
i wonder if i was right about the liquid being some sort of pre-stage sclerotia,
if that is the case, it should form a stone, well see.

notice the ring of blobs on the outer edge of the plate, looks so cute.

Quote:

Quote:

RogerRabbit said:
Try cutting off a chunk of the sclerotia itself and transferring it to a new dish.  I'll bet it takes off and grows.
RR









for my other ongoing mexa projects look here:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5702814/Main/5676075/#5702814

k, ill keep it coming :sun:


update

the story of the blob goes on:



god agar rules :wink:

Edited by ohmatic (06/05/06 02:49 PM)

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Invisiblefastfred
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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5667905 - 05/24/06 08:52 AM (17 years, 11 months ago)

I'd guess either myc piss or condensed water. Interesting post.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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Re: mex-a metabolic waste ? [Re: fastfred]
    #5668193 - 05/24/06 10:53 AM (17 years, 11 months ago)

those dishes are condensation free (finally got my pouring down heh)
so that can't be it.

myc piss, hmm, well as i already guessed it could be some metabolic byproduct thats excreted during sclerotia growth but then the question is,
why dont all dishes show it but just those two ?


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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5668209 - 05/24/06 10:58 AM (17 years, 11 months ago)

Perhaps there's a bit of bacteria in those two dishes and the metabolytes are attempting to fight it off.
RR


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semper in excretia sumus solim profundum variat

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Re: mex-a metabolic waste ? [Re: RogerRabbit]
    #5671048 - 05/25/06 12:21 AM (17 years, 11 months ago)

It could also be another fungus growing on your agar with myc. waste on it. Yellow beads of liquid are usually what I see on fungal contaminates in my media.


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Re: mex-a metabolic waste ? [Re: mycogirl]
    #5679293 - 05/27/06 01:19 AM (17 years, 11 months ago)

blobs are growing ..

liquid hasnt got a color to it, entirely looks like water ?

*edit

contam hmmmmm  :confused:


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Edited by ohmatic (05/27/06 01:30 AM)

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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5681849 - 05/27/06 10:16 PM (17 years, 11 months ago)

I dont think that stuff represents a metabolite but I could be wrong.


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Re: mex-a metabolic waste ? [Re: Zen Peddler]
    #5682265 - 05/28/06 01:06 AM (17 years, 11 months ago)

ill do something suggested by anno, transfer some of the liquid to a clean plate and see if it grows.

if it does, its prolly some bacteria / contam ?


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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5682606 - 05/28/06 05:39 AM (17 years, 11 months ago)

Has the appearance changed in the last few days? Bacteria would have covered the plate by now.
RR


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Re: mex-a metabolic waste ? [Re: RogerRabbit]
    #5683478 - 05/28/06 02:04 PM (17 years, 11 months ago)

hardly.


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RIP #cultivation! ....can't associate? well FUCK U !

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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5685631 - 05/29/06 04:33 AM (17 years, 11 months ago)

If the plate is being kept in the fridge, the bacteria won't necessarily replicate(and take over the plate). Plated cultures are stored in the fridge to slow growth to non existent so we can use the colonies for a few months.


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Re: mex-a metabolic waste ? [Re: mycogirl]
    #5685648 - 05/29/06 04:57 AM (17 years, 11 months ago)

were not even sure if it is bacteria - as it doesnt spread.

the riddle continues,
today ill transfer some blob liquid to a sterile plate, lets see if something grows.


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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5687880 - 05/29/06 07:02 PM (17 years, 11 months ago)

My point is, that if the dish in stored at ~5 C (refrigerator) it won't spread. To determine if it is bacteria, plate out the thing you are questioning, and leave it at 28-37 C. If a cloudy mass appears on the plate, you know it is bacteria.


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Re: mex-a metabolic waste ? [Re: mycogirl]
    #5689771 - 05/30/06 06:05 AM (17 years, 11 months ago)

Actually, bacteria will grow very well in the refrigerator, just a bit slower. That's why they stink so bad if we leave the leftovers from the chinese restaurant in there too long.

Try cutting off a chunk of the sclerotia itself and transferring it to a new dish. I'll bet it takes off and grows.
RR


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semper in excretia sumus solim profundum variat

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Re: mex-a metabolic waste ? [Re: RogerRabbit]
    #5689784 - 05/30/06 06:12 AM (17 years, 11 months ago)

a chunk of the sclerotia with the liquid on ?


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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5689895 - 05/30/06 07:47 AM (17 years, 11 months ago)

Yup.


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semper in excretia sumus solim profundum variat

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Re: mex-a metabolic waste ? [Re: RogerRabbit]
    #5702917 - 06/02/06 04:26 AM (17 years, 11 months ago)

update

look in main post for details.


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RIP #cultivation! ....can't associate? well FUCK U !

Edited by ohmatic (06/03/06 03:38 AM)

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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5705383 - 06/02/06 08:32 PM (17 years, 11 months ago)

What's the update?


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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Re: mex-a metabolic waste ? [Re: fastfred]
    #5706678 - 06/03/06 03:53 AM (17 years, 11 months ago)

Quote:

fastfred said:
What's the update?






look in main post for details.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

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Re: mex-a metabolic waste ? [Re: ohmatic]
    #5715354 - 06/05/06 02:49 PM (17 years, 11 months ago)

update #2


look in main post for details.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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