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OfflinePsyCode
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Registered: 02/22/06
Posts: 36
Last seen: 14 years, 8 months
115 Degrees kill germination?
    #5667477 - 05/24/06 02:10 AM (17 years, 8 months ago)

After a spore is injected, it is given 2 days before given incubation. The heating pad was on the lowest setting but the insulation wasn't sufficient (a shoe box within a thin 6 layer folded blanket). 10 hours later the thermometer read 115F degrees (the air inside the blanket, jar and content temp unknown). Did this kill all chances of germination? What is the lethal temp for colonization? And..how many days should the jars go through before opening the lids halfway to allow air exchange? Thank you


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InvisibleHorusTh3Chrous

Registered: 11/14/05
Posts: 955
Re: 115 Degrees kill germination? [Re: PsyCode]
    #5667507 - 05/24/06 02:25 AM (17 years, 8 months ago)

The same thing happened to me, next time just use room temp, with lots of blankets over.
I did 7 jars and three survived! They are actually in the chamber now! I think the three that made it were on the outer part of the heating blanket, were the heat is not as hot as the middle of it.
So yes, i would say they are toast! But i would wait two weeks.
It never hurts to start new jars, and always have a backup plan!


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Offlinekilroy
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Re: 115 Degrees kill germination? [Re: HorusTh3Chrous]
    #5667675 - 05/24/06 05:41 AM (17 years, 8 months ago)

I just posted a thing close to this ealier this week. My girlfriend put the heater on high without knowing it would hurt(I have since taught her). Out of 4 jars 2 are fighting hard and 1 is in cridical and 1 is probly dead. Give them a little time, they are a little stronger then you might think. Wait a couple of weeks but you might want to make up a couple more like Psycode said.


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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Offlinehyphae
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Registered: 12/13/02
Posts: 6,228
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Re: 115 Degrees kill germination? [Re: kilroy]
    #5667682 - 05/24/06 05:54 AM (17 years, 8 months ago)

122F (50C) is where thermal death limits are for spores, this is where they begin to degrade.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinezeegos
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Registered: 04/03/06
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Re: 115 Degrees kill germination? [Re: hyphae]
    #5667703 - 05/24/06 06:14 AM (17 years, 8 months ago)

Pitty that aint the same temp to kill all bacterial spores  :rolleyes:


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Offlinekilroy
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Re: 115 Degrees kill germination? [Re: hyphae]
    #5667722 - 05/24/06 06:28 AM (17 years, 8 months ago)

Quote:

hyphae said:
122F (50C) is where thermal death limits are for spores, this is where they begin to degrade.




I could have sworen that someone posted that thermal death could happen at like 105F. I know just because one person posted it doesnt make it so but I thought that was where it started at. Mine got up to about 105F and most look like they will survive but one I think my be resting its soul very soon, turning all gray.


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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Offlinehyphae
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Re: 115 Degrees kill germination? [Re: kilroy]
    #5668399 - 05/24/06 11:37 AM (17 years, 8 months ago)

Understanding the difference between spores and mycelium is extremely important in this hobby :wink:  BTW thermal death limits for myc have been established at 106F. This is where myc begins to degrade, duration at these temps is what will decide whether they survive or not. GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineShdwstr
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Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
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Re: 115 Degrees kill germination? [Re: hyphae]
    #5668457 - 05/24/06 11:53 AM (17 years, 8 months ago)

A couple years ago, I picked up an FSRC donation and forgot it was left in the car for a day.
When I went to get them. The temp inside the car was well into the 180 deg temps.
I was pretty sure the prints would be toast, but decided to do a little experiment.
I took 4 know good prints and put them in the oven at 180 overnight (approx 10 hrs)
Subsequent testing showed no problems with the spores viability, even at those temps.

I get a kick out of testing experiments :smile:


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Offlinehyphae
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Registered: 12/13/02
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Re: 115 Degrees kill germination? [Re: Shdwstr]
    #5669595 - 05/24/06 06:01 PM (17 years, 8 months ago)

Quote:

Shdwstr said:
A couple years ago, I picked up an FSRC donation and forgot it was left in the car for a day.
When I went to get them. The temp inside the car was well into the 180 deg temps.
I was pretty sure the prints would be toast, but decided to do a little experiment.
I took 4 know good prints and put them in the oven at 180 overnight (approx 10 hrs)
Subsequent testing showed no problems with the spores viability, even at those temps.

I get a kick out of testing experiments :smile:



Now thats interesting not really sure what to think about that as that  goes against proven scientific controlled experiments by others but theres been more than once these cubies have surprised me. That would mean you could inoculate your bulk substrates then pasturize it! I don't think I'd bite on that sorry not saying it didn't happen but it takes more than that for me.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 1 month
Re: 115 Degrees kill germination? [Re: hyphae]
    #5670621 - 05/24/06 10:32 PM (17 years, 8 months ago)

Heheheh... Yeah, I wouldn't be starting a new tek for the process anytime soon either.
But like you said... it was interesting.


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OfflinePsyCode
Stranger
Registered: 02/22/06
Posts: 36
Last seen: 14 years, 8 months
Re: 115 Degrees kill germination? [Re: Shdwstr]
    #5675749 - 05/26/06 02:16 AM (17 years, 8 months ago)

Well, it's been day 4 and the whiteness is showing, 100% germination on all jars...crazy it's happening fast considering the warm temps in my state(only 5, lol). Yes, I believe the duration of the temp was very minimal thus the results. But it's always good to know the lethal temps, not many people know about those specifics, thanks to all the people who devote their lives to putting these questions to the test, cheers!


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