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drSE
Pseudo Reality



Registered: 12/19/03
Posts: 4,432
Loc: Twighlight Zone
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Mycobags
#5666935 - 05/23/06 11:12 PM (17 years, 8 months ago) |
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I searched the posts and can't find much of anything on mycobags, just some basic info. Can you flush Mycobags if you are useing the mycobag as your fruiting chamber? Or is it just one flush and thats it?
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
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Re: Mycobags [Re: drSE]
#5666946 - 05/23/06 11:16 PM (17 years, 8 months ago) |
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Yeah you can grow invetro or use it for spawn or you can use it for bulk substrate.
Bags ROCK 
Jars are so 80's (someone said once)
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Atheist Chat
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christ_house
Stranger

Registered: 10/03/05
Posts: 47
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Re: Mycobags [Re: drSE]
#5666951 - 05/23/06 11:17 PM (17 years, 8 months ago) |
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some strains of cubensis do better than others but you can potentially get quite a few flushes.
tasmanians do great in a grain-based bag, as do the mazatapec and allen strains.
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Babo911
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Registered: 09/05/05
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I grow shiitake right out of the bag, works great if i punch holes or cut off the top
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drSE
Pseudo Reality



Registered: 12/19/03
Posts: 4,432
Loc: Twighlight Zone
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Re: Mycobags [Re: Babo911]
#5666973 - 05/23/06 11:21 PM (17 years, 8 months ago) |
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I heard that you should inject a syringe of distilled water into the mycellium after first flush to help the second flush.
Also, i just cut the bag open, pick the mushrooms, and then seal it back up, and wait for second flush?
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christ_house
Stranger

Registered: 10/03/05
Posts: 47
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Re: Mycobags [Re: drSE]
#5667326 - 05/24/06 12:48 AM (17 years, 8 months ago) |
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Quote:
drSE said: I heard that you should inject a syringe of distilled water into the mycellium after first flush to help the second flush.
Also, i just cut the bag open, pick the mushrooms, and then seal it back up, and wait for second flush?
works for me, but i don't inject into the mycelium. i just mist the bag lightly with distilled water, but the syringe idea is appealing.
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Techno_Raver
Registered: 05/05/06
Posts: 328
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I tried the GT strain in the rye based mycobags and got no results at all. it's been over 1.5 months and I haven't seen a strand of mycelium grow. I don't know if it's the spores or the grains, but it seems to me like the PF tec works better.
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christ_house
Stranger

Registered: 10/03/05
Posts: 47
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Quote:
Techno_Raver said: I tried the GT strain in the rye based mycobags and got no results at all. it's been over 1.5 months and I haven't seen a strand of mycelium grow. I don't know if it's the spores or the grains, but it seems to me like the PF tec works better.
i've never tried the rye bags but with the grain-based they must be kept between 80 and 85 degrees during colonization, and in absolute darkness.
i would assume that you would have no problem getting the rye colonized, perhaps some trouble with fruiting (?) but i'm not sure. are they being kept under the right conditions?
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
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Quote:
christ_house said: i've never tried the rye bags but with the grain-based they must be kept between 80 and 85 degrees during colonization, and in absolute darkness.
Rye IS a grain. Filter patch bags generally hold more product than jars, therefore there is more internal heat produced as the grain colonizes. Don't go over 80F during colonization of grains.
Also, light has zero effect on colonizing spawn(agar, rye, millet, wbs, etc), good or bad. I've placed colonizing petri dishes and grains on an open shelf at normal room temperature with normal indoor light exposure for many years. Once the grain is spawned to manure or cased, it's a good idea to cover with foil to restrict air exchange while allowing gas exchange, as well as to block light during the time the substrate/casing is colonizing. This allows the introduction of light to the fully colonized substrate to act as one of the pinning triggers. Just remember, light is one of the pinning triggers, but it's horribly overrated by many growers who misunderstand that it's not the only one. You still need a fully colonized substrate, an increase in fresh air, which results in a rapid decrease in CO2 levels, evaporation from the substrate or casing layer, and light.
If one wishes to grow invitro on grains in a bag, it helps to mix moistened vermiculite in with the grains prior to sterilization. This is known as rez-effect. Otherwise, I'd recommend opening the bag up and applying a casing layer to the top once it's fully colonized. You can fruit in the bag by opening the top of the bag once or twice per day to allow fresh air exchange. Remember, the filter patch will exchange a very small amount of gasses, but for good fruiting performance, you need actual air exchange, which the filter can't provide. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Mycobags [Re: drSE]
#5668435 - 05/24/06 11:45 AM (17 years, 8 months ago) |
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These were my results with bags from themadseason done invitro. These were Cambodians:
 
They flushed about 4 times. I would rather just buy empty spawn bags and make them myself though.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Mycobags [Re: drSE]
#5668448 - 05/24/06 11:51 AM (17 years, 8 months ago) |
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Quote:
drSE said: I heard that you should inject a syringe of distilled water into the mycellium after first flush to help the second flush.
Also, i just cut the bag open, pick the mushrooms, and then seal it back up, and wait for second flush?
Cut the bag open, pick the mushrooms, dunk the substrate block overnight, dab the excess water, place it back into the bag and fruit it again. Repeat this between each flush.
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johnhenry
Minderbinder
Registered: 02/22/05
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Re: Mycobags [Re: FooMan]
#5668531 - 05/24/06 12:16 PM (17 years, 8 months ago) |
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Swim agrees with fooman. Swim also adds hydrogen peroxide to distilled water to help prevent contamination. If you don't want to dunk, you can mist after each flush, works pretty well.
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zeegos
Shroomagator


Registered: 04/03/06
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ooo im doing cambodians as well. Its about 70% colonised after about 1.5 months (yea i know). Maybe its because it wasent in absolute darkness. In ur pics FooManShroom, do you keep the bag in-tact for the first flush? I was thinking its a lot of substrate and could maybe make 2 casings with it. This is my first grow so what do you recommend?
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RogerRabbit
Bans for Pleasure


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Loc: Seattle
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Re: Mycobags [Re: zeegos]
#5668926 - 05/24/06 02:10 PM (17 years, 8 months ago) |
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Quote:
zeegos said: Maybe its because it wasent in absolute darkness.
Read my post above. Light has absolutely no effect on colonizing mycelium. You don't need to keep a spawn bag in darkness. That is one of the old myco myths that is proving very hard to die, thanks to wrong information presented in Stamets' TMC. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Mycobags [Re: zeegos]
#5669002 - 05/24/06 02:43 PM (17 years, 8 months ago) |
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Quote:
zeegos said: ooo im doing cambodians as well. Its about 70% colonised after about 1.5 months (yea i know). Maybe its because it wasent in absolute darkness. In ur pics FooManShroom, do you keep the bag in-tact for the first flush? I was thinking its a lot of substrate and could maybe make 2 casings with it. This is my first grow so what do you recommend?
I kept the bags intact the whole time. You will always get better results when you case any substrate rather than letting it grow invitro. As far as it taking so long, I would adjust the temps. I agree with RR that light won't do any harm during incubation. The only reason to keep an incubating substrate in the dark is to help avoid premature pinning, not to help growth.
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drSE
Pseudo Reality



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Posts: 4,432
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Re: Mycobags [Re: FooMan]
#5669012 - 05/24/06 02:48 PM (17 years, 8 months ago) |
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Great info, I appreciate it.
So if i were to inject distilled water back intot he bag with a swyringe, how much water should i use (in CC)?
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zeegos
Shroomagator


Registered: 04/03/06
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Re: Mycobags [Re: drSE]
#5669492 - 05/24/06 05:29 PM (17 years, 8 months ago) |
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Im pretty excited about this being my first grow and all, and with this i tend to check my substrate bag/jars quite often. Could this be a possible factor for slow growth? Maybe mycelium just needs to be left alone and the temp remain constant (opening my incubator will prob result in heat loss). How often do you guys check your inoculated jars?
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Mycobags [Re: zeegos]
#5669691 - 05/24/06 06:30 PM (17 years, 8 months ago) |
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As long as you aren't touching the substrate, you shouldn't have any problems. Grab the bag from the top. Touching the actual substrate inside the bag can damage the mycelium. It will likely recover, but slow things down.
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FooMan



Registered: 02/02/05
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Loc: Earth
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Re: Mycobags [Re: drSE]
#5670176 - 05/24/06 08:35 PM (17 years, 8 months ago) |
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Quote:
drSE said: Great info, I appreciate it.
So if i were to inject distilled water back intot he bag with a swyringe, how much water should i use (in CC)?
I would dunk it. There is a way to do it by adding the weight that was lost prior to the first flush. Mainly, you want the substrate moist, but not soaking with standing water in the bag.
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Jack_Straw
ROC

Registered: 06/19/03
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Quote:
RogerRabbit said: Read my post above. Light has absolutely no effect on colonizing mycelium. You don't need to keep a spawn bag in darkness. That is one of the old myco myths that is proving very hard to die, thanks to wrong information presented in Stamets' TMC. RR
Daaaaamn! Those are harsh words... but I hope you're right, as lately someone seems to let laziness prevail and just throw jars up on the shelf with daylight hitting em.
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drSE
Pseudo Reality



Registered: 12/19/03
Posts: 4,432
Loc: Twighlight Zone
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Do I cold shock it to induce pinning once fully colonized?
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johnhenry
Minderbinder
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Re: Mycobags [Re: drSE]
#5671897 - 05/25/06 08:40 AM (17 years, 8 months ago) |
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Depends on the temperature where you are. Definitely take away heat source. In winter months SWIM got great pinning and flushes with B+ without dunking, but SWIM tells me that some strains might be more particular than others. He had no luck with getting a penis envy bag to pin and by the time he decided to cold shock the bag had gone bad.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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90% of my jars colonize in a small room with shelves.
There is a window in that room. I don't have any problems.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Mycobags [Re: drSE]
#5672196 - 05/25/06 10:30 AM (17 years, 8 months ago) |
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Quote:
drSE said: Do I cold shock it to induce pinning once fully colonized?
Some people swear by cold shocking. I have seen absolutely no benefit to doing it myself. If anything, it seemed to slow fruiting.
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inoculatedGreif
greif struken


Registered: 03/14/06
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Re: Mycobags [Re: FooMan]
#5672215 - 05/25/06 10:38 AM (17 years, 8 months ago) |
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some strains cold shock. its more of a prefreince. light will induse pinning.
-------------------- one branch of man turns away what has made us who we are, the other,excepts it for how its made man. which path do you choose? ------------------------------------- Man takes advantage of who he is,nature is his home, where he evolved from. So why does he turn it down, defy, and mutalate his birth ground? why does he spread disease, murder his native animals,rape and torture his land, and still feel descent of who he is? live a life that is not true? excepts an artificial home?
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drSE
Pseudo Reality



Registered: 12/19/03
Posts: 4,432
Loc: Twighlight Zone
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That helps alot. My strain is B+. I am on sort of a timed schedule. about 50% colonized (maybe more) in a TiT at 79degrees. <-- i think thats the right temp.
I guess i will use light to induce pinning because i can't afford it to take a really long time to fruit. I just got a job today and i am planning on movie 2 states over in 3-4 weeks. I hope thats enough time.
I hate it when you get stressed for time.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Mycobags [Re: drSE]
#5673256 - 05/25/06 02:17 PM (17 years, 8 months ago) |
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you cannot pin a 1/2 colonized jar.
one pinning trigger is a colonized substrate.
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drSE
Pseudo Reality



Registered: 12/19/03
Posts: 4,432
Loc: Twighlight Zone
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i am gonna let it get fully colonized first. I meant to mention when it got 100% colonized but i guess i forgot.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Quote:
inoculatedGreif said: some strains cold shock. its more of a prefreince. light will induse pinning.
I agree with it being a preference thing, but as for "some strains" needing it, I say
Light, temps and proper humidity will induce pinning. No strain needs cold shocking. It's another one of those things someone did a long time ago and convinced a bunch of people that it was needed or helped when in reality, the time was right for pinning anyway.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Mycobags [Re: drSE]
#5673278 - 05/25/06 02:24 PM (17 years, 8 months ago) |
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you should have just enough time to eek out a few fruits before the move.
don't only consider light for pinning. Consider all of the parameters. Relative Humidity Fresh Air Exchange Proper Moisture Content Good Evaporation off of the casing layer. Correct Temperature. Correct Exposure to Light.
There is alot to consider when fruiting, not just light. There's stuff other than this stuff too, but I worry about those ones.
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