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OfflineKryptik
Enjoy the Magic

Registered: 04/05/06
Posts: 136
Last seen: 9 years, 3 months
8 out of 9 PFTek Jars Contam..Source?
    #5666107 - 05/23/06 07:28 PM (17 years, 8 months ago)

I'm new to this, but have read as much as I can take in and consider myself  Very diligent and precise in the projects I do. I first started w/ 4 1pint Jars. I filled them with the BRF mix and added 3/4" dry verm to the tops. The 3/4" of area where the verm was added, was completely clean. The jars were PC'd for 1hour and inoculated the following morning in a glove box. I closed all 4 jars in the glove box and then steralized everything with Lysol. A fresh needle was used and the 4 jars were treated. In each of the 4 jars, Green mold started to grow within 3 days at each of the 4 injection sites on each of the 4 jars. 1 jar fully recovered very fast, but the other 3 were destroyed. Following this, I cooked 5 1/2pint jars in the same fashion, except I used as much heat as I could get out of my burners. Again, the jars were carefully inoculated and once again..all 5 developed green mold at their injection sites. I've used a very nice cigar torch to steralize the needle each time between uses. The holes in the lids are no bigger than the needle itself. Any ideas on where my source of contam is? I have not tried a control jar, but I'm pretty sure it would last a long time with no infection. Thanks for any insite! :thumbup:


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Hex45 78 70 6c 6f 72 65 20 74 68 65 20 4d 61 67 69
63 2e 20 4b 69 6c 6c 20 79 6f 75 72 20 65 67 6f 21


Edited by Kryptik (05/23/06 07:58 PM)


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: 8 out of 9 PFTek Jars Contam..Source? [Re: Kryptik]
    #5666193 - 05/23/06 07:48 PM (17 years, 8 months ago)

two sources I can narrow it down to:

your needle
the spore solution

If it is coming from the injection sites it should be one of the two. Your process sounds fine.

here: Never let the needle touch the air if you can.

http://www.shroomery.org/forums/favlinke...r=1&postmarker=


http://www.shroomery.org/forums/favlinke...r=1&postmarker=


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OfflineKryptik
Enjoy the Magic

Registered: 04/05/06
Posts: 136
Last seen: 9 years, 3 months
Re: 8 out of 9 PFTek Jars Contam..Source? [Re: monstermitch]
    #5666232 - 05/23/06 07:57 PM (17 years, 8 months ago)

I like what is written in those links and using micropore tape. I'll give that a shot next. Being new to this, I don't want to blame the solution..so onward with a fresh order and new ideas. Also going to try WBS on my next batch. Thanks Monster


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Hex45 78 70 6c 6f 72 65 20 74 68 65 20 4d 61 67 69
63 2e 20 4b 69 6c 6c 20 79 6f 75 72 20 65 67 6f 21


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: 8 out of 9 PFTek Jars Contam..Source? [Re: Kryptik]
    #5666238 - 05/23/06 07:58 PM (17 years, 8 months ago)

good luck.

don't give up. You will win in the end for sure.


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Offlinealsnow469
Stranger

Registered: 10/15/05
Posts: 392
Last seen: 6 months, 13 days
Re: 8 out of 9 PFTek Jars Contam..Source? [Re: Kryptik]
    #5667375 - 05/24/06 01:06 AM (17 years, 8 months ago)

Try PCing it longer at least 90 min or more.

That solved alot of my shroomclap problems :evil:


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