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homusubi
Curious Noob =P


Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 13 years, 10 months
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Peroxide Agar, help plz...
#5661220 - 05/22/06 06:25 PM (17 years, 8 months ago) |
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hi ppl, I was reading some post, and now I'm confused. In the post they say: "add a X% Peroxide solution to your agar wen its under 50ºC". But, it seams that X% is not really X%, because i think that they add a X% of a already existing solution and they don't mention the concentration of the original solution. The same happens to the solution to kill cobweb, or the one sprayed on the casing layer to prevent contamination.
Can someone give me the REAL concentration of the solution used.
I'm asking, because I can get some Peroxide, in different % solution, and don't wanna kill my little mycelium.
thx for the help
-------------------- "Entonces, mientras camines por esta tierra, Tu y tus hijos abrazar?is las Tinieblas, beber?is s?lo sangre, comer?is s?lo cenizas, ser?is como fuisteis en el momento de morir, nunca muriendo, siempre viviendo. Caminar?is para siempre en las Tinieblas, todo cuanto toqu?is se desmoronar?, hasta los ?ltimos d?as".
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homusubi
Curious Noob =P


Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 13 years, 10 months
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Re: Peroxide Agar, help plz... [Re: homusubi]
#5661980 - 05/22/06 09:35 PM (17 years, 8 months ago) |
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-------------------- "Entonces, mientras camines por esta tierra, Tu y tus hijos abrazar?is las Tinieblas, beber?is s?lo sangre, comer?is s?lo cenizas, ser?is como fuisteis en el momento de morir, nunca muriendo, siempre viviendo. Caminar?is para siempre en las Tinieblas, todo cuanto toqu?is se desmoronar?, hasta los ?ltimos d?as".
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Pinback
Stranger


Registered: 07/20/02
Posts: 836
Loc: Europe
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Re: Peroxide Agar, help plz... [Re: homusubi]
#5663171 - 05/23/06 06:58 AM (17 years, 8 months ago) |
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Rush Wayne, the author of the peroxide manuals, uses a final concentration of 0.018% in his agar media, but also notes that twice that concentration can be used without visible harm.
I use 0.024% which works fine.
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homusubi
Curious Noob =P


Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 13 years, 10 months
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Re: Peroxide Agar, help plz... [Re: Pinback]
#5682954 - 05/28/06 09:49 AM (17 years, 8 months ago) |
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thx  0.018% is really less than what i expected... In USA they sell 3% solution, right? that will be 1 part of Peroxide solution in 166 of water O_O
and to prevent contamination in ur casing layer, how much they use??
-------------------- "Entonces, mientras camines por esta tierra, Tu y tus hijos abrazar?is las Tinieblas, beber?is s?lo sangre, comer?is s?lo cenizas, ser?is como fuisteis en el momento de morir, nunca muriendo, siempre viviendo. Caminar?is para siempre en las Tinieblas, todo cuanto toqu?is se desmoronar?, hasta los ?ltimos d?as".
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