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OfflineDragonChaser
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Contaminated TLG syringe
    #5639420 - 05/16/06 08:51 PM (17 years, 9 months ago)

OK, I know that everyone says syringes cant be contaminated, because they are made in sterile conditions. Recently, i recieved 8 syringes from TLG. Two of them, African Transkei and Thai Elephant dung had strange brown spongy matter floating in the syringes. I recently did agar cultures of all seven strains, fourteen dishes in total. I did this in a glove box. I took the necessary precautions, flame sterilizing the syringes and rubbing in alcohol. Only four of all fourteen were contaminated. They were both TED and AT. Its only been two days since inoculation and there is strange pink matter growing on the dishes. Here are some photos. You can even see that it is growing in the pattern that the solution was sprayed onto the agar.




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My name is Mud


Edited by DragonChaser (05/16/06 09:08 PM)


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OfflineRogerRabbitM
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Re: Contaminated TLG syringe [Re: DragonChaser]
    #5639598 - 05/16/06 09:30 PM (17 years, 9 months ago)

Bacteria. I don't see any parafilm and you're handling the dishes without gloves. That alone will contaminate them with bacteria.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleRoadkillM
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Re: Contaminated TLG syringe [Re: DragonChaser]
    #5639801 - 05/16/06 10:09 PM (17 years, 9 months ago)

what RR said...



plus...

thats what you get from buying syringes from TLG.

:smirk:


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinekilroy
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Re: Contaminated TLG syringe [Re: Roadkill]
    #5639871 - 05/16/06 10:19 PM (17 years, 9 months ago)

I take it that TLG is a place to stay away from. I only do biz with ralphs and spores101


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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OfflineAnnoA
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Re: Contaminated TLG syringe [Re: RogerRabbit]
    #5639943 - 05/16/06 10:31 PM (17 years, 9 months ago)

Quote:

RogerRabbit said:
Bacteria. I don't see any parafilm and you're handling the dishes without gloves. That alone will contaminate them with bacteria.
RR





Don't you think the dark area on the rim is from whatever the dishes are wrapped with?

And, would what you are describing be the case, the contamination would be located more towards the outside of the dish, not exactly in the middle and not exactly where the spore water landed.

As for "I know that everyone says syringes cant be contaminated, because they are made in sterile conditions." I don't know who ever claimed this, but it is clearly bullshit. Every vendor has a bad batch from time to time, and every now and then, a bad batch passes the quality control, as it seems to have happened in your case.

Contact them for replacement.


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OfflineDragonChaser
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Re: Contaminated TLG syringe [Re: RogerRabbit]
    #5640083 - 05/16/06 10:55 PM (17 years, 9 months ago)

Quote:

RogerRabbit said:
Bacteria. I don't see any parafilm and you're handling the dishes without gloves. That alone will contaminate them with bacteria.
RR




The dishes were closed with tape around the edges before they even left the glove box. I don't see how handling closed and sealed dishes with my hands could contaminate them.
Quote:

Anno said:
And, would what you are describing be the case, the contamination would be located more towards the outside of the dish, not exactly in the middle and not exactly where the spore water landed.




That was my thinking exactly. The contamination is exactly where the solution landed. And if it was contamination from me, then why would only the 2 AT dishes and the 2 TED dishes be contaminated, and none of my other 12?
And how do I go about asking for replacements?
Dragon


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My name is Mud


Edited by DragonChaser (05/16/06 10:59 PM)


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OfflineAnnoA
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Re: Contaminated TLG syringe [Re: DragonChaser]
    #5640159 - 05/16/06 11:08 PM (17 years, 9 months ago)

Email them that you saw some bacteria under the microscope, and that's not what you wanted.


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OfflineDragonChaser
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Re: Contaminated TLG syringe [Re: Anno]
    #5651248 - 05/19/06 06:41 PM (17 years, 8 months ago)

I emailed the dude at TLG saying I noticed bacteria under my microscope. No reply yet. I'm getting pretty angry.
Dragon


--------------------
My name is Mud


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