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nuk1m
Dutchman


Registered: 08/23/04
Posts: 460
Last seen: 10 years, 3 months
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Bottom of jars take forever
#5650608 - 05/19/06 03:12 PM (17 years, 8 months ago) |
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Why do all my WBS jars colonize the sides first then slowly the bottom? Is it because of the all the top weight above the bottom? My incubater gets no hotter than 82 so I dont think it's the heat. I've tried laying them on there side but it doesn't seem to help much.
Edited by nuk1m (05/19/06 03:14 PM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Bottom of jars take forever [Re: nuk1m]
#5650654 - 05/19/06 03:31 PM (17 years, 8 months ago) |
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There are several possibilities one being a slightly higher moisture content because of gravity, lack of adequate gas exchange perhaps? Try cracking the lids loose a little and fipping the jars on their lids. Also are you using any tape the reason I ask is that will stall jars everytime. GL bro
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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AlteredAgain
Visual Alchemist


Registered: 04/27/06
Posts: 11,181
Loc: Solar Circuit
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Re: Bottom of jars take forever [Re: hyphae]
#5650857 - 05/19/06 04:31 PM (17 years, 8 months ago) |
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I left tape on my jars and I am incubating right now. Are you saying that the holes in the lids should be left uncovered? Wouldn't that risk contamination?
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Bottom of jars take forever [Re: AlteredAgain]
#5650882 - 05/19/06 04:38 PM (17 years, 8 months ago) |
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Not at all if your doing the PFTek if your not then you should have been using micropore tape or Tyvek with selfsealing inoculation sites.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Bottom of jars take forever [Re: AlteredAgain]
#5650888 - 05/19/06 04:41 PM (17 years, 8 months ago) |
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holes in PF (brf/verm) jars should be uncovered, because if you did it right there's a layer of dry verm on top that keeps the contams out.
holes in grain/wbs jars should be covered, but there should also be a filtered opening designed in your lid to allow the gas exhcnage.
in response to the initial poster, you can give your wbs jars a shake to redistribute the myc covered grains around thus giving you more even colonization. that's one of the biggest benefits to using grains.
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AlteredAgain
Visual Alchemist


Registered: 04/27/06
Posts: 11,181
Loc: Solar Circuit
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Re: Bottom of jars take forever [Re: hyphae]
#5650893 - 05/19/06 04:42 PM (17 years, 8 months ago) |
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Will the mycellium develop at a quicker rate once I remove the tape?
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Bottom of jars take forever [Re: AlteredAgain]
#5650897 - 05/19/06 04:43 PM (17 years, 8 months ago) |
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definitely.
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AlteredAgain
Visual Alchemist


Registered: 04/27/06
Posts: 11,181
Loc: Solar Circuit
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Re: Bottom of jars take forever [Re: creamcorn]
#5650907 - 05/19/06 04:47 PM (17 years, 8 months ago) |
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Thanks for the advice.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Bottom of jars take forever [Re: creamcorn]
#5650916 - 05/19/06 04:51 PM (17 years, 8 months ago) |
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Quote:
creamcorn said: definitely.
Most definitely
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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