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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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InvisibleOlgualion
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agar observation.
    #564707 - 02/27/02 10:07 AM (21 years, 7 months ago)

I have noticed that two petri dishes(half-pints in this case) with the same medium filed to different depths show very different growth speed. The same tamp isolate was used, and the one with the deeper agar is much slower. Has anyone noticed this? if so is there an optimum depth that the agar should be poured?


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Offlineuniboner
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Re: agar observation. [Re: Olgualion]
    #564794 - 02/27/02 12:20 PM (21 years, 7 months ago)

Well i guess it makes sense.
Instead of the isolate colonizing horizontally on shallow agar, my possibly twisted sense of logic dictates that deeper agar would encourage the isolate to colonize vertically as well as horizontally, thereby seeming to slow down the overall growth rate when infact it is most likely the same, but divided by another dimention.

Theoretically, and nonsensically speaking ofcourse..

peace,
uniboner


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Edited by uniboner (02/27/02 12:30 PM)

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InvisibleShaw

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Re: agar observation. [Re: Olgualion]
    #564865 - 02/27/02 02:36 PM (21 years, 7 months ago)

it has more to substrate to colonize, so it would make sense that it would take longer.


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InvisibleOlgualion
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Re: agar observation. [Re: Olgualion]
    #564889 - 02/27/02 02:56 PM (21 years, 7 months ago)

Whenever I have seen mycelium on agar it has always been growing on the surface. I have never seen it grow through the agar(at least it doesn't appear to). But even if this were the case, the growth should be radial (proper usage?) from the beginning point. IOW, I believe it would grow at the same rate across the surface if it were 2D or 3D. So I think that if my observation is right, there is a different reason for it. Or maybe it was just this particular wedge.


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Offlineuniboner
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Re: agar observation. [Re: Olgualion]
    #564947 - 02/27/02 04:19 PM (21 years, 7 months ago)

I think you're right.
I don't know what could be causing this discrepancy.


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OfflineSeussA
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Re: agar observation. [Re: uniboner]
    #565037 - 02/27/02 06:16 PM (21 years, 7 months ago)

Make a list of every possible thing that is different.  Things like which jar your poured first.  Which jar you innoculated first.  The position of the jars in the incubator.  The amount of mycelium originally transfered per jar.  The location the original mycelium came from.  Order your list from what you feel is the most likely to least likey cause.  Change the first thing on your list and see if the results change.  Eventually, if you care enough, you will figure out what the difference is.  :smile:

My initial guess would be a difference in the temperature of the jars due to the proximity of the heater or differing convection paths.

Are you sure you have a single isolate?  Differing genetics could be to blame.

Interesting question... good luck figuring out what is up!


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OfflineAzure
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Re: agar observation. [Re: Seuss]
    #565253 - 02/27/02 09:59 PM (21 years, 7 months ago)

Think of agar as a sponge filled with water and nutrients. The molarity(concentration) may be the same in both samples, but the one with deeper substrate has more total amount of nutrients if the concentrations are the same. This may not be the reason, but it is definitely a factor that should be explored. Also, the one with a deeper substrate has less volume of gas in the dish. HOwever, some deep dishes have shown better growth than shallow dishes.
Also, if the nutrient rich solutes were not mixed thoroughly before the plates were poured, one plate could have more nutrients than another.


Edited by Azure (02/27/02 10:02 PM)

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