Home | Community | Message Board


Magic-Mushrooms-Shop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Jump to first unread post. Pages: 1
InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Trusted Cultivator
agar observation.
    #564707 - 02/27/02 12:07 PM (15 years, 4 days ago)

I have noticed that two petri dishes(half-pints in this case) with the same medium filed to different depths show very different growth speed. The same tamp isolate was used, and the one with the deeper agar is much slower. Has anyone noticed this? if so is there an optimum depth that the agar should be poured?


--------------------
Study the past...
See the future...


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineuniboner
member
Registered: 07/19/01
Posts: 173
Loc: TO
Last seen: 2 years, 5 months
Re: agar observation. [Re: Olgualion]
    #564794 - 02/27/02 02:20 PM (15 years, 4 days ago)

Well i guess it makes sense.
Instead of the isolate colonizing horizontally on shallow agar, my possibly twisted sense of logic dictates that deeper agar would encourage the isolate to colonize vertically as well as horizontally, thereby seeming to slow down the overall growth rate when infact it is most likely the same, but divided by another dimention.

Theoretically, and nonsensically speaking ofcourse..

peace,
uniboner


--------------------
sleep is for the weak.


Edited by uniboner (02/27/02 02:30 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShaw
hick

Registered: 06/28/01
Posts: 2,218
Loc: midwest, usa
Last seen: 18 days, 1 hour
Re: agar observation. [Re: Olgualion]
    #564865 - 02/27/02 04:36 PM (15 years, 3 days ago)

it has more to substrate to colonize, so it would make sense that it would take longer.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Trusted Cultivator
Re: agar observation. [Re: Olgualion]
    #564889 - 02/27/02 04:56 PM (15 years, 3 days ago)

Whenever I have seen mycelium on agar it has always been growing on the surface. I have never seen it grow through the agar(at least it doesn't appear to). But even if this were the case, the growth should be radial (proper usage?) from the beginning point. IOW, I believe it would grow at the same rate across the surface if it were 2D or 3D. So I think that if my observation is right, there is a different reason for it. Or maybe it was just this particular wedge.


--------------------
Study the past...
See the future...


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineuniboner
member
Registered: 07/19/01
Posts: 173
Loc: TO
Last seen: 2 years, 5 months
Re: agar observation. [Re: Olgualion]
    #564947 - 02/27/02 06:19 PM (15 years, 3 days ago)

I think you're right.
I don't know what could be causing this discrepancy.


--------------------
sleep is for the weak.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 23 days
Re: agar observation. [Re: uniboner]
    #565037 - 02/27/02 08:16 PM (15 years, 3 days ago)

Make a list of every possible thing that is different.  Things like which jar your poured first.  Which jar you innoculated first.  The position of the jars in the incubator.  The amount of mycelium originally transfered per jar.  The location the original mycelium came from.  Order your list from what you feel is the most likely to least likey cause.  Change the first thing on your list and see if the results change.  Eventually, if you care enough, you will figure out what the difference is.  :smile:

My initial guess would be a difference in the temperature of the jars due to the proximity of the heater or differing convection paths.

Are you sure you have a single isolate?  Differing genetics could be to blame.

Interesting question... good luck figuring out what is up!


--------------------
Just another spore in the wind.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 9 months
Re: agar observation. [Re: Seuss]
    #565253 - 02/27/02 11:59 PM (15 years, 3 days ago)

Think of agar as a sponge filled with water and nutrients. The molarity(concentration) may be the same in both samples, but the one with deeper substrate has more total amount of nutrients if the concentrations are the same. This may not be the reason, but it is definitely a factor that should be explored. Also, the one with a deeper substrate has less volume of gas in the dish. HOwever, some deep dishes have shown better growth than shallow dishes.
Also, if the nutrient rich solutes were not mixed thoroughly before the plates were poured, one plate could have more nutrients than another.


Edited by Azure (02/28/02 12:02 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* bohemica pinheads on agar Una 4,158 15 06/18/01 07:55 PM
by amanita
* Re: a few questions for an agar guru... gray1 1,953 8 05/06/01 05:18 PM
by gratefulredhead
* Agar Strain Isolation - Is it worth it?
( 1 2 all )
Blue Helix 5,245 20 02/23/05 03:07 AM
by Blue Helix
* Best pre-made agar plates for Cultivation no-tone 2,238 9 01/30/03 12:31 PM
by no-tone
* Is this the right "Agar Agar" I guess?????
( 1 2 all )
NTTG_Hunter 1,908 25 10/17/03 05:16 PM
by NTTG_Hunter
* Need help with agar Peace_Patrol 785 2 07/15/02 05:43 AM
by zeronio
* Beer Agar.... GabbaDj 1,477 10 08/20/02 08:38 PM
by danelectro
* No growth on agar. skroom 1,115 18 11/07/03 02:22 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
919 topic views. 0 members, 7 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The Spore Depot
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.002 seconds on 14 queries.