Innoculating with agar wedge observations - Mushroom Cultivation

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homebrew
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Registered: 06/02/01
Posts: 117
Last seen: 20 years, 18 days

Innoculating with agar wedge observations

#564651 - 02/27/02 08:44 AM (21 years, 3 months ago)

After my failed birdseed attempt with agar wedges (I think it was lost to bacteria because of a sour fermentation stench), I am following Anno's "Grain for Dummies" tek (using rye berries). Prior to innoculation, I shook the jars again and then put my agar wedges mycellium side down on top of the grain. This was done about 48 hrs ago. I took a peek at them today and am seeing no signs of mycellium entering the rye (as of yet), but the mycellium is visibly growing on the previously uncolonized agar side (which didn't happen with my lost jars upon post mortem examination). I've got my fingers crossed that this time will be successful. I am curious that perhaps I should have put the wedges in colonized side up, so that the newly colonized agar face would be on top of the rye berries. Only time will tell.

On a side note, a friend innoculated some brf jars with agar wedges in a similar fashion and is not seeing colonization after more than a week, should he shit can and start over? I don't know what advice to give him as we are both new to agar.

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cookiewhore
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Registered: 01/03/02
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Re: Innoculating with agar wedge observations

[Re: homebrew]
#564881 - 02/27/02 02:51 PM (21 years, 3 months ago)

Well, i inoculate from agar slabs. usually after 24 hours the mycelium has grown off the agar onto the rye grain.
did you shake the rye grain up after autoclaving? i find after sterilization, and cooling process, the top dries (or looks dry) and it needs a good shake

Also, i usually throw about 4 slabs in, then shake it up (carefully) you dont wnana get the agar stuck to the side of the jar. try rolling it, not really shaking. just to get it into the rye.

ocne you get the rye colonized.. . go with spawn

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homebrew
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Registered: 06/02/01
Posts: 117
Last seen: 20 years, 18 days

Re: Innoculating with agar wedge observations

[Re: cookiewhore]
#565747 - 02/28/02 11:16 AM (21 years, 3 months ago)

This AM (72 hrs) there is nice white mycellium all over the agar and it is starting to enter the rye. I am full of confidence that all will be successful. To cookiewhore: I believe that the slowness issue is a factor of the temperature at which the jars are allowed to sit. Currently, the room fluctuates from 65-75 F and I don't feel like investing in an incubation chamber yet (maybe I will today). They are colonizing, so I ain't gonna sweat it for now.

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Seuss
Error: divide byzero

Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 5 months, 15 days

Re: Innoculating with agar wedge observations

[Re: homebrew]
#565873 - 02/28/02 01:32 PM (21 years, 3 months ago)

Those temps will really slow down the growth to almost nothing (at 65F).

--------------------
Just another spore in the wind.

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homebrew
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Registered: 06/02/01
Posts: 117
Last seen: 20 years, 18 days

Re: Innoculating with agar wedge observations

[Re: Seuss]
#566630 - 03/01/02 08:00 AM (21 years, 3 months ago)

Still seeing increases in mycellium growth over 24 hr intervals, but I guess I will bite the bullet and build an incubation chamber this weekend and hopefully the jars will pick up the pace. I estimate at their current rate, the jars will take about a month to fully colonize. Thanks for the input guys.

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