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Offlineflatalbert
MexicanaJalisco,Stamets, andfractals
Registered: 01/30/02
Posts: 109
Loc: One of those corn states
Last seen: 8 years, 11 months
Mexi A preliminary experiments
    #564590 - 02/27/02 09:04 AM (14 years, 9 months ago)

I have been doing experiments on the growth of the Mexi A strain in honey water in a 19:1 water: honey proportion. Variables included, light, heat and sugar content. When fruiting the honey jars in rye seed grain cased with coir, I will check for growth rate or mutations/abhorts. This was done with 2 jars for each factor (heat, lack of heat, light lack of light, 2 control jars, etc.) The house was kept at 72 degrees.
Light: Mycelium grew faster than control jar. Microscope viewing of the mycelia showed no difference. Lack of light caused the jar to grow slower. Bio-assay will be conducted to find if light causes higher growth, but lower potency.
Heat: jar also grew faster with the presence of heat, but mycelia shows a more white color than the light jar. No microscopic difference percieved.
Sugar content: High sugar content in the ratio of 20:3 stored in the dark with no extra heat showed sluggish growth compared to the control. Yellowed mycelia under the microscope, but I think that may be from the honey or too much nutrients. Weak looking under the microscope similar to degenerated strain mycelia. Lack of sugar in a 40:1 ratio showed lack of growth. Few bits of mycelia. White mycelia, healthy, just less.
I am going to create a normal honey jar and let it run out of sugar to see if sclerotia form.
Questions to be answered: What initiates sclerotia production? Can I do this in the honey jar using these factors?
Will any factors cause mutations or improved growth on rye seed cased with coir?
Any additions, comments, or ideas are welcome.
Peace
Flatal


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Offlineretardricky
member
Registered: 01/10/02
Posts: 178
Loc: hanover park Il
Last seen: 12 years, 27 days
Re: Mexi A preliminary experiments [Re: flatalbert]
    #564595 - 02/27/02 09:09 AM (14 years, 9 months ago)

That is the strain that does not produce fruits right. And I know its a stupid question but whats that sclerotia shit.


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Offlineflatalbert
MexicanaJalisco,Stamets, andfractals
Registered: 01/30/02
Posts: 109
Loc: One of those corn states
Last seen: 8 years, 11 months
Re: Mexi A preliminary experiments [Re: flatalbert]
    #564635 - 02/27/02 10:29 AM (14 years, 9 months ago)

I forgot to say that it is the Mexicana A strain from Sporeworks, it was done with multispore innoculation.

To answer the previous reply, psilocybe mexicana DO produce smaller potent fruits than cubensis. The A strain produces more sclerotia and the B strain produces more carpophores. They both fruit.
If you want to know what sclerotia are, do a search here at the Shroomery. You will turn up some good information.

Any questions, comments, ideas, or critiques concerning my experiment?

Peace
Flatalbert


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Offlinedendro
journeyman
Registered: 03/05/99
Posts: 4
Last seen: 4 years, 1 month
Re: Mexi A preliminary experiments [Re: flatalbert]
    #564964 - 02/27/02 06:44 PM (14 years, 9 months ago)

Hya flatalbert, I saw a post at another site that described a simple liquid tek
that used h2o2, malt and water in a sterile container, but air was passed thru h2o2 and then over the surface of the liquid and then out a backflow preventer valve, once a day only. The poster claimed if left a while, the mycelium grew into a solid block filling the container! Anyone heard of this?


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Offlineflatalbert
MexicanaJalisco,Stamets, andfractals
Registered: 01/30/02
Posts: 109
Loc: One of those corn states
Last seen: 8 years, 11 months
Re: Mexi A preliminary experiments [Re: dendro]
    #565097 - 02/27/02 09:31 PM (14 years, 9 months ago)

Yeah, I have heard of it. It was from my good buddy Azarius at Spiritplants.com. I am not trying to create mycelia for Mexi A, I am trying to test the growing parameters to see if I can make growth better or worse for the sclerotia and fruits of this strain.
What initiates sclerotia? Could I do this in the jar?
Workman? Anno? Anyone?
Peace
Flatal


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Anonymous

Re: Mexi A preliminary experiments [Re: flatalbert]
    #565221 - 02/27/02 11:16 PM (14 years, 9 months ago)

The same thing that usually stimulates fruiting, lack of available nutrition in the remaining substrate. Fruiting also usually includes a more specific set of environmental factors to coincide with nutrient deprivation. Sclerotia form when the conditions are not optimum for fruiting, at a higher rate, but will still form within this tight range.

Initiate the A-strain invitro on rye grass seed. The B-strain will produce sclerotia in the casing layer of your fruiting trays, but not invitro.


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