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LochRaven
mycophile


Registered: 01/31/06
Posts: 190
Last seen: 13 years, 3 months
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parafilm alternative?
#5645370 - 05/18/06 07:26 AM (18 years, 5 days ago) |
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are there any good parafilm alternatives? i would be using them for test tubes and petri dishes...
-------------------- there are rainbows and earthworms and microscopic hummingbirds www.elestialsound.com
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
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Re: parafilm alternative? [Re: LochRaven]
#5645372 - 05/18/06 07:32 AM (18 years, 5 days ago) |
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So far, I've tried nearly all the plastic wraps, micropore medical tape and masking tape. None can compare with parafilm. You want gas exchange, but no air exchange. Micropore tape gives too much exchange and the dishes dry out. The Saran type wraps either don't allow enough gas exchange, or none at all, resulting in slow growth. Masking tape doesn't stop contaminants. I'd get parafilm. It only costs a few pennies per dish to wrap and does the job well. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: parafilm alternative? [Re: RogerRabbit]
#5645440 - 05/18/06 08:01 AM (18 years, 5 days ago) |
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Use cotton or pollyfill to plug your tubes. You can take a roll of saran wrap and cut it into rolls about the size of packing tape. The main reason to wrap is to seal out contams and prevent the medium from drying out too quickly. Parafilm is a lot better like RR said, but it is kind of pricey.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 16 hours
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Re: parafilm alternative? [Re: LochRaven]
#5645699 - 05/18/06 09:52 AM (18 years, 5 days ago) |
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Glad wrap polyethylene film cut in 1 inch roles is all I have been using for the last 5 years.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 days
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Re: parafilm alternative? [Re: Anno]
#5645917 - 05/18/06 11:24 AM (18 years, 5 days ago) |
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Quote:
Anno said: Glad wrap polyethylene foil cut in 1 inch roles is all I have been using for the last 5 years.
Works perfect to might I add! This is the only gas permeable plastic wrap that I know of (Glad Cling Free).
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
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Re: parafilm alternative? [Re: hyphae]
#5646666 - 05/18/06 03:09 PM (18 years, 5 days ago) |
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Try inoculating two side by side dishes with the polyethylene on one and parafilm on the other. What I found was both cultures would grow pretty evenly for the first week, then the polyethylene one would slow down, while the parafilm wrapped dish kept growing rapidly. Neither contaminated however, so the Glad does its job that way. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 16 hours
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Re: parafilm alternative? [Re: RogerRabbit]
#5649111 - 05/19/06 04:24 AM (18 years, 4 days ago) |
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I would see this as an advantage, you have to plate less often, or?
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LochRaven
mycophile


Registered: 01/31/06
Posts: 190
Last seen: 13 years, 3 months
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Re: parafilm alternative? [Re: Anno]
#5653683 - 05/20/06 03:29 PM (18 years, 3 days ago) |
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thanks for the very useful information. i think im going to start off using polyethylene and once i get some more cash flow flowing i might upgrade to parafilm.
-------------------- there are rainbows and earthworms and microscopic hummingbirds www.elestialsound.com
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 days
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Re: parafilm alternative? [Re: LochRaven]
#5654745 - 05/20/06 10:19 PM (18 years, 2 days ago) |
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Quote:
LochRaven said: thanks for the very useful information. i think im going to start off using polyethylene and once i get some more cash flow flowing i might upgrade to parafilm.
GLAD Cling Wrap is what you want.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
Edited by hyphae (05/21/06 09:37 AM)
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 16 hours
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Re: parafilm alternative? [Re: hyphae]
#5655646 - 05/21/06 08:55 AM (18 years, 2 days ago) |
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I think it's "GLAD Cling Wrap"
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 days
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Re: parafilm alternative? [Re: Anno]
#5655696 - 05/21/06 09:36 AM (18 years, 2 days ago) |
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Quote:
Anno said: I think it's "GLAD Cling Wrap"
I was having a brainfart sorry guys
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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deanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: parafilm alternative? [Re: hyphae]
#5671529 - 05/25/06 04:04 AM (17 years, 11 months ago) |
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Have you tryed paraffin wax ? It works 4 me
Edited by deanofmean (05/25/06 04:17 AM)
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jeffpro
panoptic
Registered: 05/14/06
Posts: 15
Last seen: 17 years, 4 months
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Re: parafilm alternative? [Re: deanofmean]
#5685046 - 05/28/06 11:09 PM (17 years, 11 months ago) |
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parafilm can be found relatively cheap if you shop around
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