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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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cooling question
#5643521 - 05/17/06 08:20 PM (17 years, 8 months ago) |
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i almost always have trouble with contam. im not to sure why. my sterile techniques are good, i always PC for a full hour. i think i might have figured out my prob though. when im done PCing, i pull the valve on thr PC. the hot air comes rushing out and cool ait in. cool air that caries contams. then i open the sucker up and take the jars out and let them cool for a few hours in a bag before inoculating them. i was thinking that just leaving them in the PC until they com down to room tem and then taking them out would be better so that they aren’t sucking air in. what do you guys think?
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: cooling question [Re: redshadow]
#5643543 - 05/17/06 08:26 PM (17 years, 8 months ago) |
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Yes you answered your own question quite well it's all a matter of physics. Pros wait pros have patients.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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MFKDGAF
Stranger


Registered: 09/01/05
Posts: 194
Loc: Midwest
Last seen: 1 year, 7 months
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Re: cooling question [Re: redshadow]
#5643549 - 05/17/06 08:27 PM (17 years, 8 months ago) |
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Yeah you should wait like 4 hours after you PC them. Especially so the jars don't crack because of temperature change.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: cooling question [Re: MFKDGAF]
#5643602 - 05/17/06 08:38 PM (17 years, 8 months ago) |
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Not to mention that when you pop the weight, the material in your jars is at 250F. Since water can't exist in a liquid state above 212F, the only thing that can happen is for the moisture to turn into steam and puke right out of your jars, leaving them dry. Never pop the weight on a pc to release pressure. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: cooling question [Re: hyphae]
#5643609 - 05/17/06 08:40 PM (17 years, 8 months ago) |
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Quote:
hyphae said: Yes you answered your own question
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Quick WBS Prep
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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Re: cooling question [Re: FooMan]
#5644020 - 05/17/06 10:16 PM (17 years, 8 months ago) |
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sweet thanks guys!
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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Re: cooling question [Re: redshadow]
#5644152 - 05/17/06 10:39 PM (17 years, 8 months ago) |
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is it ok to inoc them right after i take them out? its been about 3 hours and the the PC has been at room temp for a few hours now.
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
Edited by redshadow (05/17/06 11:14 PM)
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Optx
PronouncedAwp-Tiks


Registered: 03/28/05
Posts: 977
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Re: cooling question [Re: redshadow]
#5644759 - 05/18/06 01:23 AM (17 years, 8 months ago) |
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no! even though the outside feels cool to the touch, the core is definitely hotter. patience is a virtue.
-------------------- please do not take everything i say here so personally. welcome to teh internets!
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
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Last seen: 2 years, 9 months
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Re: cooling question [Re: Optx]
#5645643 - 05/18/06 09:24 AM (17 years, 8 months ago) |
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well... i ended up waiting a full 4 hours, and inco'ing them. im worried though. they were warm when i took them out. hot hot at all, barley even warm... but warmer then room temp.
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: cooling question [Re: redshadow]
#5645657 - 05/18/06 09:35 AM (17 years, 8 months ago) |
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Seems like patience is a problem for you. Waiting for the jars to cool completely won't take long compared to waiting for dead spores to germinate. If your jars felt warm to the touch, they were probably too hot for your spores. Oh well, patience can't be taught, it has to be learned.
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Quick WBS Prep
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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Re: cooling question [Re: FooMan]
#5645867 - 05/18/06 11:11 AM (17 years, 8 months ago) |
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they didn’t feel that warm, im pretty sure they’re ok, but who knows. i guess ill have to wait a week or so and we'll see. i incoed'ed a few jars long a go for my first grow and the jars were warmer, much warmer. it worked. i must say i did have beginner’s luck on that grown. ill keep everyone posted on my new jars....
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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Re: cooling question [Re: redshadow]
#5645973 - 05/18/06 11:44 AM (17 years, 8 months ago) |
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i thought 4 hours is a pretty good time to wait? how long should i wait?
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: cooling question [Re: redshadow]
#5645976 - 05/18/06 11:45 AM (17 years, 8 months ago) |
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I wait overnite.
I like the jars to almost feel cool or coldish. no fridge or anything.
just sitting out on the table overnite.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: cooling question [Re: redshadow]
#5647138 - 05/18/06 05:42 PM (17 years, 8 months ago) |
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Quote:
redshadow said: well... i ended up waiting a full 4 hours, and inco'ing them. im worried though. they were warm when i took them out. hot hot at all, barley even warm... but warmer then room temp.
You have to remember spores will start to deteriorate at 122F (50C) so there is a buffer zone but also keep in mind jars that feel warm are most likely over 100F as your body temp is 98.6 and surface temp is roughly 88F plus so a little warm may be alright but it's best not to rush this hobby IMHO. Either way GL
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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redshadow
Yaddia Holla?


Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
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Re: cooling question [Re: hyphae]
#5647169 - 05/18/06 05:56 PM (17 years, 8 months ago) |
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yeah not rushing is always the best way. i usually wait over night, but im always so busy. i just go a new job that keeps me occupied and i commute another 4. 13hr of my 24 hour day is sucked up trying to make damn living. fuck the system!
-------------------- =<{: Its only when you've lost everything that your free to do anything....... :}>=
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