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Offlinegarygenn69
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someone pleASE HELP Mny tries all contanination or dont fruit
    #5637202 - 05/16/06 12:14 PM (17 years, 8 months ago)

Look Guys I followed everything to the letter But I am keep getting cont from somewhere usuaklly black,grteen mold, the fuzzy white mold snd pin mold. I bought new two pressure cookers made a nice glovebox with a hepa filter and fan, did the martha stewart groww closet with humidity at opttimum and temp within spec. I dont want to give up I wash my hads then use germ x i was using lysol for surfaces andthis air santizer (forget name OHHHHH OUST. ) But the only thing I can think is that is not working well and maybe i should turn to bleachi did convert part of my basement as a lab that is confined in plastic and a hepa filter that runs any way Does aNYONE HAve anything I can try please enIL NE EMil/reply to post


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InvisibleCureCat
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Registered: 04/19/06
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Re: someone pleASE HELP Mny tries all contanination or dont fruit [Re: garygenn69]
    #5637251 - 05/16/06 12:31 PM (17 years, 8 months ago)

What substrate are you using? Grain or verm/brf, other?? Grain such as rye should be soaked in water for 24hrs before you cook it and put it into jars for sterilization. There are heat resistant endospores in some grain esp. rye berries, that are easier to kill if they are triggered to germinate by soaking in water before you cook them.

How long and at what psi are you pressure cooking?? Optimum psi is 15, and I recommend letting it boil in there for 60-90min. Some people say 20, but I think that is asking for trouble (maybe they are just really lucky) and so i like to leave no chance for bad things to grow because i didn't PC long enough.

Are you sterilizing your needle after each onnoculation? hit it with a lighter for a few seconds (water should sizzle out the tip) and then take a cotton swab soaked in isopropyl alcohol and wipe it down- DO NOT touch the needle!

You're not opening the jars during colonization, right?? This might be a little over board, but i wear a face mask to avoid breathing all over my jars.

Any other details you can give us?


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OfflineRogerRabbitM
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Re: someone pleASE HELP Mny tries all contanination or dont fruit [Re: garygenn69]
    #5637405 - 05/16/06 01:05 PM (17 years, 8 months ago)

Quote:

garygenn69 said:
made a nice glovebox with a hepa filter and fan




Most likely your problem right there. Take the fan off, seal up the hole and go to work. Gloveboxes work by having still air. Turbulent air will swirl contaminants into your projects.
RR


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Offlineeuphrates
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Re: someone pleASE HELP Mny tries all contanination or dont fruit [Re: RogerRabbit]
    #5637514 - 05/16/06 01:28 PM (17 years, 8 months ago)

if you do use the fan, i would assume you want it sucking the air out. make sure you crub the box down every time you use it.


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