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Invisibleohmatic
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Registered: 02/28/04
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grain to grain transfer - how do YOU do it
    #5636264 - 05/16/06 02:21 AM (17 years, 8 months ago)

ey !

well, i just spottet the mean green in 9 jars i g2g'd couple days ago,
bummer, but what shall you do.

it was the first time i g2g'd, so well, its not to bummin.
anyway, i opened the recieving jars and poured in grains from the master
in the flowhood, well, thats what your supposed to do no ?

only htiong i can think of is that somehow contams from the outside of the masterjar
got blown into the reception jar somehow as i was to close when pouring out grains maybe ?
next time i do this ill wipe down the outside of the master jar with alochol aswell.

i know some people use spoons (remember a post of agar) uhm, well,
so how do you do it ?

oh and i know the flowhood isnt the source of contamination as all agar work i did is contam free.

well, just a pic of the jars for fun:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/16/06 03:23 AM)


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Offlinenife
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Re: grain to grain transfer - how do YOU do it [Re: ohmatic]
    #5636331 - 05/16/06 03:34 AM (17 years, 8 months ago)

LC is the way to go for my money. Its harder to contam. You can use a syringe. And you can be pretty sure its not contamed before you inject it since its growing mycelium. And its almost as fast as g2g.


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Invisibleohmatic
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Re: grain to grain transfer - how do YOU do it [Re: nife]
    #5636348 - 05/16/06 03:45 AM (17 years, 8 months ago)

Quote:

nife said:
LC is the way to go for my money. Its harder to contam. You can use a syringe. And you can be pretty sure its not contamed before you inject it since its growing mycelium. And its almost as fast as g2g.




please refrain from suggesting different teks and answer my question,
if you can't, don't.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineHippieChick
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Re: grain to grain transfer - how do YOU do it [Re: ohmatic]
    #5636397 - 05/16/06 04:51 AM (17 years, 8 months ago)

Don't know how you messed up . I've done all mine in a glovebox and never lost one . ????

Peace,Love,Happinesas and Harmony
:heart: Hippie Chick  :mushroom2:


--------------------
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InvisibleTomandjerry58
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Re: grain to grain transfer - how do YOU do it [Re: HippieChick]
    #5636435 - 05/16/06 05:32 AM (17 years, 8 months ago)

get in a closet or some kind or tight spot in ur house. with no carpet ofcourse. mask, gloves,lysol, spoon h202 in a glass. lots of lysol in the air. open colonized jar, open pre colonized jar. take spoon out of glass with h202 scoop a couple scoops out and put in pre colonized jar. put spoon back into glass with h202. repeat. with one quart jar you can do about 12 jars. they will finish in about 8 days. and don't wory the hydrogen peroxide it won't hurt it.

the reason i think people use lc is because its easier and more resistant to contam because ur not opening the jar ofcourse.

hope i helped, this is not my tek i learned from a guy named poops a couple yrs ago it works great

one thing you can do is make a glove box or just work out of a tub


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Invisibleohmatic
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Re: grain to grain transfer - how do YOU do it [Re: HippieChick]
    #5636437 - 05/16/06 05:35 AM (17 years, 8 months ago)

Quote:

HippieChick said:
Don't know how you messed up




i dont know either, i think i got a little idea abou a possible contam vector but im not sure.

Quote:

preschooler said:
one thing you can do is make a glove box or just work out of a tub




read my post ? i got a laminar flowhood.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleTomandjerry58
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Re: grain to grain transfer - how do YOU do it [Re: ohmatic]
    #5636447 - 05/16/06 05:41 AM (17 years, 8 months ago)

thats great! i wish i had one. never used one before.
i forgot to write make sure u close jar after each transfer. and wait till ur done to shake. im not saying that you don't know this ohmatic but we do have alot of newbs reading too.


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OfflineCrazyPsilocybe
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Re: grain to grain transfer - how do YOU do it [Re: Tomandjerry58]
    #5636460 - 05/16/06 05:49 AM (17 years, 8 months ago)

I wipe down the outside of the jars after having the same thing happen to me, and I started wearing gloves after I saw the pic of the petri dish someone posted on here from "washed" hands. But I think it came from the outside of the master jar.
Preschoolers got a point too, wanna limit exposure time.
It's why we call it the mean green, huh.


--------------------
"I hate to advocate drugs, alcohol, violence or insanity to anyone, but they've always worked for me."
-Hunter S. Thompson


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InvisibleTomandjerry58
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Re: grain to grain transfer - how do YOU do it [Re: Tomandjerry58]
    #5636461 - 05/16/06 05:52 AM (17 years, 8 months ago)

another thing. ur jars up there have too much grain in them you only wont to fill them about half way. u need to have shaking room. this is one of the most important things. so you can evenly distribute the grains after transfer. this is for speed!!!! and the faster you get them colonized the better chances without contams


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InvisibleHolydiver
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Re: grain to grain transfer - how do YOU do it [Re: ohmatic]
    #5636475 - 05/16/06 06:02 AM (17 years, 8 months ago)

That is strange, I've done lots of G2G's in a still-air glovebox, and haven't seen any contams in ages. Only two things I really do are 1) wipe down the work surface and gloves w/ alcohol, then clean the spoon that I use for transfers w/ alcohol. No need to sterilize the tools in my experience, as long as alcohol is applied liberally.


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Invisiblegotyerback
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Re: grain to grain transfer - how do YOU do it [Re: Holydiver]
    #5636611 - 05/16/06 07:55 AM (17 years, 8 months ago)

Try sterilizing a bag of grain. wbs is cheap and easy. Spray your pint jars with alcohol. I mean spray the crap out of the whole jar. Keep your jars in the flowhood while the alcohol evaporates. Open up the bag and dump the whole jar in the bag. Close up the bag. Twist-tie works fine. Smash the spawn up and mix it around. In a week or two you have several quarts of grain spawn in that bag.

This is not the most sterile way to do it. I don't know why contams don't eat up the bag, maybe because the way the spawn was mixed in there, the shrooms just get to it first.


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OfflineOatman2000
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Re: grain to grain transfer - how do YOU do it [Re: gotyerback]
    #5636625 - 05/16/06 08:01 AM (17 years, 8 months ago)

I shake my master jar... till it's back to kernel state.

then remove all outer rings to all jars. my ratio is about 7:1 master jar. (stamets says you can make 10)

quickly and in OPEN AIR (no glovebox) i take the lid completely off the master, and dump, close.... to the other g2g jars.

here's a pic 4 days later...


--------------------
Spawning to COIR
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WBS QUART SPAWN JAR PREPERATION
----------------------------

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Invisiblemycijor
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Registered: 04/12/06
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Re: grain to grain transfer - how do YOU do it [Re: Oatman2000]
    #5636728 - 05/16/06 08:48 AM (17 years, 8 months ago)

Before I start I mist the whole room from the ceiling down with a weak bleach solution. Just water will do too . The objective is to clean dust out of the air and make any other dust stick to the walls and ceiling. I use clear glass bottles for masters. (Old Miller beer bottles.) I transfer outside of the glove box on a clean counter. I keep the master bottle mouth close to an alcohol lamp between transfers. I use an old milk carton with a slot cut in it to hold the master bottle in front of the alcohol lamp. I just came out of the shower and I do not wear anything. I never used HEPA filters and never had a contamination problem. The masters are inoculated inside a glove box. The master bottle is shaken to break up the grain. To prevent clumping I add a quarter teaspoon of ground oyster shell in each spawn master. One spawn master will inoculate about 14 quart jars. I sterilize a pair of hot pads with the spawn batch that I use to lift the jars out of the pressure cooker so I can minimize touching the jar lids.


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