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OfflineHUBSonDUBS
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Registered: 09/01/05
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Making water sterile for syringes
    #5622796 - 05/12/06 08:55 PM (17 years, 8 months ago)

Can I just PC a 1/2 pint jar full of tap water with tinfoil on top for an hour and then wait over night to cool and scrape the spores off of the tinfoil with a sterile knife into the water?


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InvisibleAtheist
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Registered: 01/24/06
Posts: 13,705
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Re: Making water sterile for syringes [Re: HUBSonDUBS]
    #5622804 - 05/12/06 08:57 PM (17 years, 8 months ago)

Why not use bottled water? I think its sterile right?


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Offlineskeletor
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Registered: 09/06/05
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Re: Making water sterile for syringes [Re: Atheist]
    #5622820 - 05/12/06 09:00 PM (17 years, 8 months ago)

boil 15 mins then put some plastic wrap over it. let cool. then suck upthrough the plastic. oh flame adn wipe that needle fist. also don't need to let cool if its a blank syringe for well whatever.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineHUBSonDUBS
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Re: Making water sterile for syringes [Re: skeletor]
    #5622837 - 05/12/06 09:03 PM (17 years, 8 months ago)

Well I actually need to put the spores in the water first so I'm going to need to let it cool, ya i know how to make the syringes sterile by sucking up boiling water for a bit Ive done that before.


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InvisibleAtheist
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Re: Making water sterile for syringes [Re: HUBSonDUBS]
    #5622853 - 05/12/06 09:06 PM (17 years, 8 months ago)

Make sure the syringe is sterile too. boil that shit


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Offlinehyphae
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Re: Making water sterile for syringes [Re: Atheist]
    #5622942 - 05/12/06 09:28 PM (17 years, 8 months ago)

Tip: Put a drop of JetDry in that water before sterilizing it that will not only keep spores easily dispersed but it will also break the waters surface tension allowing spores to sink and not just sit on top of the water.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleOptx
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Registered: 03/28/05
Posts: 977
Re: Making water sterile for syringes [Re: hyphae]
    #5623020 - 05/12/06 09:45 PM (17 years, 8 months ago)

the way i make syringes is super simple.
1. boil Brita filtered water (but regular tap water should be fine also)
2. suck up the boiling water into the syringe, let it sit in there for 3-5 seconds. then shoot the water out and repeat until you're happy(i do this probably 15 times or so)
3.suck up a final syringe full of water and let it sit somewhere clean for about 2 hours to completely cool down.
4. wipe down a shot glass with 91% isopropyl alcohol. (for extra sterility i lay the alcohol soaked paper towel on top of the shot glass to keep germs out)
5. wipe down razor with alcohol, scrape spores into the shot glass
6. shoot in the sterile water from the cool syringe, stir slightly with the needle, then suck the spore water in.
7. pat yourself on the back and be sure to let the spores rehydrate for 24+ hours before innoculating your substrate.


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please do not take everything i say here so personally. welcome to teh internets!


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