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OfflineLearning2Much
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Registered: 08/25/05
Posts: 86
Last seen: 17 years, 8 months
Poo Questions?
    #5621576 - 05/12/06 03:12 PM (17 years, 8 months ago)

Can a Colonized Poo cake be added to water to create a syringe to inoculate wbs. I have done it with PF Cakes, but not sure if you can do it with Poo???

***


Can you add pasteurized poo to a poo cake that is app. 80-90% colonized to add to the over-all size of the poo cake?

***

Looking forward to here your input!!!!

Thks

L2M


Edited by Learning2Much (05/12/06 03:44 PM)


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Invisiblemonstermitch
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Re: Poo Questions? [Re: Learning2Much]
    #5621587 - 05/12/06 03:15 PM (17 years, 8 months ago)

:vineclimb:


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OfflineLearning2Much
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Re: Poo Questions? [Re: monstermitch]
    #5621616 - 05/12/06 03:23 PM (17 years, 8 months ago)

?


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OfflineLearning2Much
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Re: Poo Questions? [Re: Learning2Much]
    #5621662 - 05/12/06 03:37 PM (17 years, 8 months ago)

Bump...

Any know know or Try is before??


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Invisiblemonstermitch
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Re: Poo Questions? [Re: Learning2Much]
    #5621666 - 05/12/06 03:39 PM (17 years, 8 months ago)

colonized poo cake in water as an innoculant?
---don't think I've ever heard of it, and I'm guessing no.

wbs like pf cakes?
---don't think I've ever heard of that quite either. Choose one or the other.

can you add new poo to an uncolonized poo cake to make it bigger?
---let the poo cake colonize to 100%, birth it, crumble it up, mix it with pasteurized poo, place in tray, cover and vent, re-incubate to 100% colonization.


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OfflineLearning2Much
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Re: Poo Questions? [Re: monstermitch]
    #5621693 - 05/12/06 03:48 PM (17 years, 8 months ago)

Monster Thanks for your input..

Would you break the colonized cake up for faster colonization of the "new" pasteurized poo?

Why would you need to wait until it is 100% colonized? I am a bit confused as to the reason why?

:shrug:


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Invisiblemonstermitch
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Re: Poo Questions? [Re: monstermitch]
    #5621696 - 05/12/06 03:49 PM (17 years, 8 months ago)

I think I'm confused, yup the monster is confused...

so...

you've taken a brf cake piece, put it into water, aspirated it into a syringe, and used that to inoculate wbs... right?

now you want to do the same thing but with a poo cake instead of a brf cake... right?

Also you just want to put the new poo in the jar with the colonizing poo? Or you want to birth the colonizing poo and mix it with more for bulk?


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Invisiblemonstermitch
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Re: Poo Questions? [Re: monstermitch]
    #5621709 - 05/12/06 03:56 PM (17 years, 8 months ago)

I have a thread here about birthing cakes early, why or why not.

the conclusion of everyone was basically whatever is not colonized has to be thrown away and cannot be used.

my assumption (that no one would answer on comment on) was the the pasteurization method leaves some "good" bacteria behind to protect it, while sterilizing leaves the substrate open to contams with no protection, it is has to be discarded.

You can spawn now if you take the colonized parts and dunk them in a 10% peroxide to distilled water solution prior to breaking them up.

So...  to avoid wasting your sterilized poo colonizing in the cake and to further protect against contams wait for the cake to colonize 100% and then birth it.
(or just do it now and do the clean it off and peroxide thing)

next crumble the cake really well and mix it with the pasteurized poo in a sterile container. 

take the pasteurized poo/broken up poo cake mix and place in tray or whatever.  Level it out.

cover, vent, and re-colonize the substrate to 100%.

case, and re-incubate until the mycelium pokes through.

fruit!!

:mushroomgrow:


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OfflineLearning2Much
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Re: Poo Questions? [Re: monstermitch]
    #5621728 - 05/12/06 04:00 PM (17 years, 8 months ago)

Quote:

monstermitch said:
I think I'm confused, yup the monster is confused...

so...

you've taken a brf cake piece, put it into water, aspirated it into a syringe, and used that to inoculate wbs... right? 

now you want to do the same thing but with a poo cake instead of a brf cake... right?




Yep!!  Just not sure if you can do that?    :thumbup:

Quote:


Also you just want to put the new poo in the jar with the colonizing poo?  Or you want to birth the colonizing poo and mix it with more for bulk? 




More your first comment... Want to add the new poo to the colonizing poo that is in the incubator to add to the bulk at the incubation stage in tupperware.  No jars, no FC yet..     


I hope that is a bit more clear..                      Any IDeas??

:cool:


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OfflineLearning2Much
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Re: Poo Questions? [Re: monstermitch]
    #5621745 - 05/12/06 04:06 PM (17 years, 8 months ago)

Quote:

monstermitch said:
I have a thread here about birthing cakes early, why or why not.

the conclusion of everyone was basically whatever is not colonized has to be thrown away and cannot be used.

my assumption (that no one would answer on comment on) was the the pasteurization method leaves some "good" bacteria behind to protect it, while sterilizing leaves the substrate open to contams with no protection, it is has to be discarded.

You can spawn now if you take the colonized parts and dunk them in a 10% peroxide to distilled water solution prior to breaking them up.

So...  to avoid wasting your sterilized poo colonizing in the cake and to further protect against contams wait for the cake to colonize 100% and then birth it.
(or just do it now and do the clean it off and peroxide thing)

next crumble the cake really well and mix it with the pasteurized poo in a sterile container. 

take the pasteurized poo/broken up poo cake mix and place in tray or whatever.  Level it out.

cover, vent, and re-colonize the substrate to 100%.

case, and re-incubate until the mycelium pokes through.

fruit!!

:mushroomgrow:




:confused:

I think I'm confused now, lol        :laugh:

Okay, PF Cakes have already been spawned to Pasteurized Poo.  Just want to use the Poo Cake to Spawn to more Poo..   

Ideas??


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OfflineBPJohnny
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Re: Poo Questions? [Re: Learning2Much]
    #5621754 - 05/12/06 04:08 PM (17 years, 8 months ago)

i dont see why not


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Invisiblemonstermitch
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Re: Poo Questions? [Re: Learning2Much]
    #5621761 - 05/12/06 04:10 PM (17 years, 8 months ago)

well, if you've pulled that off with brf, then go ahead with the poo. mycelium is mycelium no matter what substrate it is growing on. I would reccomend other ways of inoculation like cloned shroom water, liquid culture, or homemade spore syringes.

Hmmm... not so much any more clear.

you have poo colonizing in the incubator right now at 80-90%, right?
what type of container is this in? I'm thinking a jar (cake0...

whatever container it is in, do not add anything to it at all.
let it colonize than take it out, crumble it up, and mix with pasteurized poo. This mixture is now your "bulk substrate"

take your bulk substrate and put it into the final fruiting container, wether that be a tray or a double tub or a monotub.

continue from there.


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Invisiblemonstermitch
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Re: Poo Questions? [Re: monstermitch]
    #5621770 - 05/12/06 04:13 PM (17 years, 8 months ago)

okay...

brf cake to poo.

now the poo via the cake wants to go to more poo.

yeah, make it bigger, keep on a going.

try not to do this too much though. Each new spawn leaves a window for contams to enter. Try sticking to grain/cake to bulk and fruit the bulk.


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OfflineBPJohnny
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Re: Poo Questions? [Re: monstermitch]
    #5621775 - 05/12/06 04:13 PM (17 years, 8 months ago)

Never mind to slow


Edited by BPJohnny (05/12/06 04:14 PM)


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OfflineLearning2Much
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Registered: 08/25/05
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Re: Poo Questions? [Re: monstermitch]
    #5621789 - 05/12/06 04:18 PM (17 years, 8 months ago)

I'll let you know how it works with the Poo cakes using this tek. I have never tried to do LC's or Cloned shroom water. I see it used alot, just never tried it, never clearly understood it.

If yo have a few good links you know of for LC's or Cloned shroom water that are simple. Please let me know..

*****

The poo in the tupperware is 80-90 percent colonized. Just want to add more poo to it to bulk it up a bit while its sitting in the incubator. Just want to know if you can use poo like you use wbs for spawn?

And if so, how does it compare for colonization time? I would guess faster?? Don't know


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OfflineLearning2Much
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Re: Poo Questions? [Re: Learning2Much]
    #5621794 - 05/12/06 04:20 PM (17 years, 8 months ago)

Yep, Monster thats the idea atleast !!!


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Poo Questions? [Re: Learning2Much]
    #5621828 - 05/12/06 04:38 PM (17 years, 8 months ago)

spawn is spawn.

kind of. 

it will provide nutes for the bulk substrate to draw from to allow it to recover faster, but basically most spawn is just used as an innoculant in solid form.

poo will work just as wbs does... in theory.

For liquid culture I suggest you either follow agar's method or blue helix's.  I started with agar's and switched to blue helix's because ther is better air exchange and it works faster for me.

shroom water is simple.  I had lots of links and everything saved then my hard drive failed and I lost it all.  Sorry.

As for the poo in the tupperware, If you're bulking it up a good amount I guess go ahead.  If you only want to add a little bit to it, I advise strongly against it.  Let it do it's thing.

Liquid culture is just light malt and dextrose in water that has been pressure cooked for 15 minutes and inoculated with a touch of spores or whatever.  Simple and easy to understand.

Shroom water is a bit harder to make.  You have to clone a shroom.  In a glovebox you take a piece of flesh from the inside of a shroom and put it into a jar full of sterilized water and a couple of drops of jet dry.  You place the blade assembly of an oysterizer blender on as a lid and secure with a band.  The jar, blade assembly, band, and future lid are are sterilized together before this process and set into a glove box where this work is done.  Once this jar is made, you blend the shroom flesh into the water and put the jar back into the glove box and switch out the blade assembly with the "future lid" I previously mentioned.  this lid is a normal metal lid (or plastic one) with a hole in it sealed with rtv to make a self healing injection port.  Or a vacutainer top could be used.

So yeah i bet I should have saved my breath on all of the shroom water stuff.  This site holds all of your answers.


Go with liquid culture.  Agar's baby bottles, blue helix's setup, or agar's regular setup.  That's my suggestion.

damn... :bongload:


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Offlinezeegos
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Re: Poo Questions? [Re: monstermitch]
    #5694799 - 05/31/06 12:47 PM (17 years, 7 months ago)

How long can pasturized poo be kept 'good' for?
I just pasturized today and its day 4 of my LC and only tiny specs appearing. Is it ok to store this poo for a few days (week max)?
Also, im wondering what kind of sterile measures need to be taken (if any)??


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