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OfflineXbollweevilX
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I feel like giving up
    #5618091 - 05/11/06 04:58 PM (17 years, 8 months ago)

I am new to cultivation and had never seen or attempted a grow until I came across this site. After reading for a few months I decided it was time to get my feet wet.

I started with the PF TEK. I followed the directions exactly and had absolutely no results. No mycelium let alone contaminants grew in my jars. I know your thinking that if I followed the directions this is not possible, but trust me, I followed them word for word.

So after my frustration settled I decided what the hell I have the supplies why not try again. I couldn't do anything different with the PF TEK since I did it right the first time so I went with Doc's wild bird seed TEK. Again following the directions exactly I had not even a thread of mycelium.

Before you say it is my spores I bought four different strains from two different vendors each found on the shroomery sponsor list. I know the only logical answer would be I didn't follow directions but I'm very meticulous about everything that I do.

Any advice would be greatly appreciated. What would have kept the mycelium from even starting?


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There is no such thing as a civilian.


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Offlineskeletor
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Re: I feel like giving up [Re: XbollweevilX]
    #5618131 - 05/11/06 05:07 PM (17 years, 8 months ago)

injection ports have made my life a million time easyer. just use some caulking or silicon over a hole. especially with grain then you inject though this and your needle actually never touches anything inside. i still flame sterilize then wipe with alcohol though.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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Offlinesplifner180
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Re: I feel like giving up [Re: skeletor]
    #5618167 - 05/11/06 05:15 PM (17 years, 8 months ago)

While I realize it's frustrating (I, myself am currently on my second grow after a first completely unsuccessful attempt), if you want us to tell you what you might have done wrong, you're going to have to give us a very detailed account of what you actually did.

We can't possibly tell you anything useful based on the information provded so far.

Not trying to be a dick. You sound rightously frazzled and I'm sure people here would love to help.

splif


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First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlineskeletor
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Re: I feel like giving up [Re: splifner180]
    #5618188 - 05/11/06 05:20 PM (17 years, 8 months ago)

some times it takes a few attempts. but let me tell you when you finally do grow its fucking satisfying much more so then it would have been if it were easy haha.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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Offlinedudefromaz
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Re: I feel like giving up [Re: skeletor]
    #5618201 - 05/11/06 05:24 PM (17 years, 8 months ago)

Hey man, don't give up, did you use a pressure cooker? Maby your spores are fucked....? Well, when i feel shitty, i just down a couple of oipum pills... :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill: :chillpill:




peace man


--------------------
Remember, the best drug, is a natural drug.....

Peace, Fratirnityishly, Smoke Da Stuff, Special Chocolate




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Offlinedudefromaz
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Re: I feel like giving up [Re: dudefromaz]
    #5618211 - 05/11/06 05:26 PM (17 years, 8 months ago)

um yea...My first grow was shitty....2pf 1 pint jars....1 contammed, 1 decent, until i tried to case it....then mr green raped them...

My current grow was the same...! good jar 1 bad... This time ima k.i.s.s. it up ...



peace


--------------------
Remember, the best drug, is a natural drug.....

Peace, Fratirnityishly, Smoke Da Stuff, Special Chocolate




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InvisiblePsychoslut
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Re: I feel like giving up [Re: XbollweevilX]
    #5618262 - 05/11/06 05:42 PM (17 years, 8 months ago)

The only thing I can think of is that you inoculated before the jars were completely cool and the spores were destroyed.

How long did you let the jars cool before inoculating?
What was the incubation temperature, or room temp if you didnt use and incubator.

Or there is a very slim chance that you got 4 bunk syringes. They don't mean to do it but Ive gotten a couple bad syringes from shroomery vendors.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineOatman2000
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Re: I feel like giving up [Re: Psychoslut]
    #5618280 - 05/11/06 05:48 PM (17 years, 8 months ago)

and they will definately make up for it most likely.. just shoot the vendor an e-mail


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: dudefromaz]
    #5618282 - 05/11/06 05:48 PM (17 years, 8 months ago)

If the contam came from areas away from the inoculation you probably didn't sterilize good enough, try pressure cooking at 15 psi for 1 hour.

If the contam is on top of the jar and/or at inoculation points you should be more sterile when innoculating. Agar has a great open air inoculation tek for those lazy or without a glove box or flowhood.

If there is a heavy contam, high water content to promote bacteria, or too high of a temperature while incubating than the mycelium wont stand a chance at all and maybe never grow.

If the contam is only where the spores were you have a bad needle or contents of syringe, probably needle. Vendors here are usually really good with their products.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: skeletor]
    #5618302 - 05/11/06 05:56 PM (17 years, 8 months ago)

Yes I used a pressure cooker. I let them sit inside the pressure cooker sealed for 24 hours before inoculating. They are sitting in a TiT incubator at 85 degrees. It has been almost 8 days now and still no growth.

Just for the consolation that it wasn't anything that I did, it would be nice if it were the syringes. I guess I'll get ahold of the vendors after a few more days and tell them what happened. Maybe they would send more. I'm willing to try again.


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There is no such thing as a civilian.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: monstermitch]
    #5618319 - 05/11/06 06:01 PM (17 years, 8 months ago)

No I haven't had any contam and I PCd at 15 psi for 90 minutes. Jars look the same as when they came out of the cooker. No contam and no mycelium.


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There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: XbollweevilX]
    #5618329 - 05/11/06 06:02 PM (17 years, 8 months ago)

so where did the contam develop from in the jar???


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: monstermitch]
    #5618347 - 05/11/06 06:05 PM (17 years, 8 months ago)

okay, my bad, I don't know where I came up with contams from...

I'm far too stoned today.

:getstoned:

well the jars could have overheated.  If the tit was set at 85 degrees inside than you probably didn't do that either.  Sometimes syringes get nuked or something in shipping, happend to me once...

dead rocket.  Sorry man, now I don't believe I can explain why no nothing.  Usually it is a heat issue somewhere along the line.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: monstermitch]
    #5618358 - 05/11/06 06:07 PM (17 years, 8 months ago)

I know that this isn't the post but I saw your signature. What do you think of the new album?


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There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: XbollweevilX]
    #5618384 - 05/11/06 06:13 PM (17 years, 8 months ago)

I think highly of it.

I have been a decipher' of their music since the early 90's and was expecting just such an album.

To me it feels like this album was easier to make for them than the others.

I like it quite a bit, but so far not my favorite, we'll see after another year of study.

Nice and heavy in parts and soft in others, like expected, the vocals seem more in the background while Adam is more in the forefront. Good over all.


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OfflineDr_Mcgillicuddy
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Re: I feel like giving up [Re: XbollweevilX]
    #5618387 - 05/11/06 06:14 PM (17 years, 8 months ago)

XbollweevilX you are having some Hard times thats to bad man it sucks dosen't it but you know those mushrooms are just waiting for you to grow them


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InvisibleAtheist
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Registered: 01/24/06
Posts: 13,705
Loc: USA
Re: I feel like giving up [Re: Dr_Mcgillicuddy]
    #5618405 - 05/11/06 06:18 PM (17 years, 8 months ago)

Listen, PM ur sponsors and they will almost definitely take care of you. I'm not promisin that but pretty sure.

Next time make an LC too.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: Atheist]
    #5618430 - 05/11/06 06:29 PM (17 years, 8 months ago)

YES LIQUID CULTURE
:dogpile:


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OfflineXbollweevilX
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Re: I feel like giving up [Re: XbollweevilX]
    #5618446 - 05/11/06 06:33 PM (17 years, 8 months ago)

I'm trying to be as optimistic as possible here and have been reading that in some cases colonization can take 12 days. Its been 8 so im going to wait 2 or 3 more days.

Mitch I had no idea what to expect and was suprised by the sound. But I too love the album.


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There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: XbollweevilX]
    #5618467 - 05/11/06 06:37 PM (17 years, 8 months ago)

wait, you might like it more. Read the lyrics and keep listening...

take your jars and put them away and forget about them for at least a week or three.

Buy new jars if you are going to start over. Jars are cheaper than throwing all of your work away do to haste and impatience.


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: I feel like giving up [Re: monstermitch]
    #5618468 - 05/11/06 06:38 PM (17 years, 8 months ago)

Quote:

monstermitch said:
YES LIQUID CULTURE
:dogpile:




No dont do a LC untill you get really good. I thought I was ready for LC's after I did my successfull bulk poo grow. I was wrong. My lc gave every jar I used it on Green mold.


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: mikeownow]
    #5618496 - 05/11/06 06:43 PM (17 years, 8 months ago)

protecting against contams is not something to wait until you get pro to do.

every noob should learn that first.

every noob can pull off lc with no problems.

lc is no harder than the pf tek in my book.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: mikeownow]
    #5618504 - 05/11/06 06:45 PM (17 years, 8 months ago)

I have other jars that have been used for stuff but are clean. What about washing them really good and PCing them empty instead of buying new jars?


--------------------
There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: XbollweevilX]
    #5618517 - 05/11/06 06:48 PM (17 years, 8 months ago)

yup, don't even have to pc them empty.

I soak jars in a 10% bleach solution for a couple of hours and then rinse and wash with antibacterial dish soap.

Never had any problems with contams due to dirty jars or substrate.


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Offlineskeletor
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Re: I feel like giving up [Re: XbollweevilX]
    #5618531 - 05/11/06 06:52 PM (17 years, 8 months ago)

no just wash them. you are goign to PC them with teh substrate anyways. by definition you are sterilizing them so you i guess don't even need to wash them haha but do wash them...no reason to be gross.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: monstermitch]
    #5618561 - 05/11/06 06:59 PM (17 years, 8 months ago)

I should at least see a contam if I were to open a jar up and scrape away the verm filter right?

Just wondering because I wanna do it to see if anything will grow on the substrate. If mold is suppose to grow on the substrate and doesn't, I guess that narrows my problem down. At this point I'm not worried about wasting one jar. But it should grow right?


--------------------
There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: I feel like giving up [Re: XbollweevilX]
    #5618592 - 05/11/06 07:05 PM (17 years, 8 months ago)

yeah skeletor is probably right, the bleach thing is just me being overly cautious, there is no real backing for that practice.

Mold will grow on your substrate. You can be 100% sure of that.

If you really just want to see something grow and are willing to give a cake to mold to see something, than go ahead.

This is your experience.


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InvisiblePsychoslut
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Re: I feel like giving up [Re: XbollweevilX]
    #5618624 - 05/11/06 07:14 PM (17 years, 8 months ago)

how long did you pc for? if you pced a lil too long the substrate could be dry and that would slow colonization or completely hinder it.

you SHOULD see growth in 5-7 days but 12 isn't something to really get worried about let them go a few more days before you open them and all that.


if they don't show growth within a few days i can send you a syringe that i know for a fact is full of viable spores  :wink:


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: I feel like giving up [Re: Psychoslut]
    #5618634 - 05/11/06 07:16 PM (17 years, 8 months ago)

that is a nice offer Psychoslut...

XbollweevilX, how can you turn that one down?


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
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Re: I feel like giving up [Re: monstermitch]
    #5618830 - 05/11/06 08:09 PM (17 years, 8 months ago)

How come no one has mentioned that he "may" have pushed the substrate in the jar and its to compac that is the most likely senario


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineXbollweevilX
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Re: I feel like giving up [Re: monstermitch]
    #5618839 - 05/11/06 08:13 PM (17 years, 8 months ago)

That is very thoughtful. I will keep you updated the next few days. Keeping my fingers crossed.

I've read on these message boards through a few searches that it has taken some peoples cakes quite a long time before first signs of colonization.

I hope thats the case. The jars got PCd for 90 minutes. After they were done I left them inside the PC for 24 hours. After wiping the outside of the PC I took it to my "clean room" and inoculated one by one pulling them from the PC. Is there any problems doing it this way? Do they need to cool down outside of the PC?

I went ahead and took the lid off one, eleven left, and finger checked the moisture. As far as I can tell the moisture level is fine, but then again I'm definitely no expert.


--------------------
There is no such thing as a civilian.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: I feel like giving up [Re: XbollweevilX]
    #5618848 - 05/11/06 08:17 PM (17 years, 8 months ago)

Yet another reason to leave them alone and forget about them. Usually one of two things will happen:

you will be happy (mycelium)
you will be sad (contam)

If you wait say, three weeks, you should be assured of one or the other. I wouldn't even begin to be concerned until it has been a month, then start thinking dud syringe.


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OfflineCrazyPsilocybe
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Re: I feel like giving up [Re: monstermitch]
    #5619095 - 05/11/06 09:21 PM (17 years, 8 months ago)

It could be a moisture issue in the jars. Also, did you shake up the syringe real well? Was there a bubble inside to stir the spores? Go to earthstongue.com (we need to get them on the sponsers!) and try theirs if you keep having probs - 20 cc syringes, full of spores and I got growth in 3 days at 76-79 deg. I did 12 pint jars of rye flour and vermiculite and 3 jars of popcorn. With the 12 pint jars I tapped 6 down on the counter while i was filling them up to compact the substrate a little, and 6 without doing that. The compacted ones are slightly slower, but not much. The ones I didn't tap down are getting noticeably skinnier as they colonize the substrate. I PC'd for 1 hr, 45 min for everything and haven't lost any to contams.


--------------------
"I hate to advocate drugs, alcohol, violence or insanity to anyone, but they've always worked for me."
-Hunter S. Thompson


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