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Offlinesplifner180
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Should Spores Be Visible in Syringe?
    #5617989 - 05/11/06 04:40 PM (17 years, 8 months ago)

I've obtained two syringes from a reputable Shroomery vendor. The first batch did nothing, no idea why. The second batch just got knocked up last last night so there is no data.

Both syringes were 100% crystal-clear with nothing at all (visible) floating inside. Is that normal?

tia - splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
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InvisibleTaskenti
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618001 - 05/11/06 04:42 PM (17 years, 8 months ago)

Thats perfectly normal....Spores ARE microscopic ya know?


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Invisiblemonstermitch
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Re: Should Spores Be Visible in Syringe? [Re: Taskenti]
    #5618020 - 05/11/06 04:46 PM (17 years, 8 months ago)

Should be okay. The vendors refer to them as "crystal" syringes and they work great for most everyone.

The darker syringes may slow down growth (not proven) but are generally viewed just as a waste because the clear ones work just as well.


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OfflineDr_Weird
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618022 - 05/11/06 04:46 PM (17 years, 8 months ago)

One of my syringes was crystal clear but had a few really tiny black clumps of spores. I shook up the syringe before using it, but it should work without that. Mine seemed to grow just fine. How long ago did you inoculate the first batch? It takes about 2-5 days to see the first faint area of mycelium growing.


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Offlinesplifner180
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Re: Should Spores Be Visible in Syringe? [Re: Dr_Weird]
    #5618037 - 05/11/06 04:49 PM (17 years, 8 months ago)

Well, I recycled all but two of the jars from the first try. I've kept those around "just in case." I call them my "legacy jars." Damned IT background. Those were done about 13 days ago. No life of any kind, good or bad. I emptied six jars from that run and all of them smelled fine with no growth. I'm using WBS, BTW.

This run started late last night so it isn't exactly surprising that there is no growth yet. I was just concerned because I've seen plenty of syringes with visible specks in them and neither of my syringes had anything visible whatsoever.

I can take failure. It's just driving me nuts to not have any idea where the process went wrong, ya know?

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlineskeletor
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Re: Should Spores Be Visible in Syringe? [Re: Taskenti]
    #5618042 - 05/11/06 04:50 PM (17 years, 8 months ago)

1000 to the tip of a pin. good spore syringes use jet try or sex lub to keep those spores from clumping anyways so they will actually be less clear since you won't see clumps. my homemade syringes i see clumps haha


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InvisibleTaskenti
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Re: Should Spores Be Visible in Syringe? [Re: skeletor]
    #5618090 - 05/11/06 04:58 PM (17 years, 8 months ago)

How long after pc'ing did you inject?


--------------------
CIVIC IS Mycoshack!! Be careful trading with him!!
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Offlinesplifner180
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Re: Should Spores Be Visible in Syringe? [Re: skeletor]
    #5618104 - 05/11/06 05:00 PM (17 years, 8 months ago)

The first batch I waited 22 hours. This batch it was more like 12. I checked the temp by laser and they were 80F or below. And it's BFR so I shook the piss out of those jars. No superhot center lurking.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlinetrentallica
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Re: Should Spores Be Visible in Syringe? [Re: skeletor]
    #5618170 - 05/11/06 05:16 PM (17 years, 8 months ago)

" sexlube" what are you talking about. so when i get to the point of making my own syringe i should plop a little KY in the mix?


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Offlineskeletor
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Re: Should Spores Be Visible in Syringe? [Re: trentallica]
    #5618241 - 05/11/06 05:35 PM (17 years, 8 months ago)

if you want. or just jet dry. thats probably cheaper. sex stuff is kinda expensive sometimes cause they have you by the well balls.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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Invisiblemonstermitch
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Re: Should Spores Be Visible in Syringe? [Re: skeletor]
    #5618255 - 05/11/06 05:40 PM (17 years, 8 months ago)

there is a good chance nothing went wrong with the legacy run and things just were developing slowly.

If you don't inject the spores onto the glass you might wait 3-4 weeks to see growth.

I have a set of quinoa cakes colonizing with a cloned water solution for innoculant that took 16 days before I even saw one tiny little speck of mycelium at all. A week later they were booming.

I guess I can suggest getting more jars instead of recycling because the first ones just might make it if left alone long enough.

I hope this run works much quicker for you.


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Offlinesplifner180
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618482 - 05/11/06 06:41 PM (17 years, 8 months ago)

monstermitch wrote:
"there is a good chance nothing went wrong with the legacy run and things just were developing slowly. If you don't inject the spores onto the glass you might wait 3-4 weeks to see growth. I have a set of quinoa cakes colonizing with a cloned water solution for innoculant that took 16 days before I even saw one tiny little speck of mycelium at all. A week later they were booming. "

You definitely could be right about that but then the problem becomes moisture content. When they began there was no pooling in the 1 pt jars but individual grains sticking to the glass had a "water halo" around them which, if I understand properly, is perfect. My lids have two 5/32" holes drilled in, one piece of micropore over one hole and two pieces over the other (the second added after innoc). The lid, of course, is attached with the jar ring and I rubberband a tyvek cover over the whole job. These jars are placed into an enclosed container (started with a Sterilite, had problems with maintaining incubation temps, moved to an Igloo (see sig)).

After almost two weeks the jars are more like maracas than wet grains.

Do I leave them be? Add sterilized water via syringe? I think I could pull that off without introducting contams if necessary.

I should also mention that one of my original 9 jars was an LC that exhibited zero growth.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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InvisibleHSIHd
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618520 - 05/11/06 06:49 PM (17 years, 8 months ago)

Quote:

splifner180 said:
The first batch I waited 22 hours. This batch it was more like 12. I checked the temp by laser and they were 80F or below. And it's BFR so I shook the piss out of those jars. No superhot center lurking.

splif




You shook the piss out of BRF jars? You only wanna shake WBS. Shaking BRF will compact it and make it harder for the myc. to spread through the substrate.


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Invisiblemonstermitch
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618566 - 05/11/06 06:59 PM (17 years, 8 months ago)

After almost two weeks the jars are more like maracas than wet grains.

So they are dry... the lid set up seems to me like it would not promote excessive drying. Not quite getting why that happened unless the pc was opened too quick but that doesn't apply here...

I have never injected dry jars. I have some fairly dry jars right now that are colonizing but just a bit slower. I guess personally I would just wait unless I could find some good info that would assure me that injecting some water would help.


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Invisiblemonstermitch
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Re: Should Spores Be Visible in Syringe? [Re: monstermitch]
    #5618581 - 05/11/06 07:02 PM (17 years, 8 months ago)

Seeming more and more like the syringe was a dead rocket though. The LC should have produced. What type of jar and/or lid setup did you use for the lc?


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Offlinesplifner180
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Re: Should Spores Be Visible in Syringe? [Re: monstermitch]
    #5618651 - 05/11/06 07:21 PM (17 years, 8 months ago)

HSIHd writes:
"You shook the piss out of BRF jars? You only wanna shake WBS. Shaking BRF will compact it and make it harder for the myc. to spread through the substrate."

Wups. I did say BFR, didn't I? So many bloody acronyms.

No, I have WBS.

monstermitch writes:
"Seeming more and more like the syringe was a dead rocket though. The LC should have produced. What type of jar and/or lid setup did you use for the lc? "

Exact same lid setup as I used for the others.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5618660 - 05/11/06 07:25 PM (17 years, 8 months ago)

with lc I always either have a lid like agar's or one like blue helix's.

agar's is spill proof and that is a bonus

blue helix's allows more air exchange and I have better results with them but have to be a tad bit more careful when drawing lc out of the jars.

how did you plan on getting the lc out of the jar in a sterile manner without tipping the jar upside down and spilling the lc out of the holes? Just tipping to the side I guess?

I would suggest a self healing injection port like rtv to make it easier.


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OfflineCrazyPsilocybe
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Re: Should Spores Be Visible in Syringe? [Re: monstermitch]
    #5619454 - 05/11/06 11:09 PM (17 years, 8 months ago)

Monstermitch - I posted this a couple days ago - may help. After injecting the water, the growth took off. I had to adjust my BRF recipe for altitude and humidity (3000+ ft. and 20-30% RH).



It could just be the dry vermiculite used for the top layer, but the slow growth makes it sounds like the cakes are a little dry. This happened to me a month ago and I luckily was able to inject more water with fantastic results. I was still able to get 12 out of 12 jars, I reinjected distilled water (15 ml=1 TBSP.) by following all original evironmental sterilization teks - wore mask, gloves and wiped the jar lids down with lysol. This is where I got creative, I mixed a sol of 91% isop. with a 2 sec. spray of lysol to wipe down the needle after every jar. I'd dip paper towel in the mix, wipe the needle, then wipe the needle with a dry portion so no liquid got mixed.

The problem is, I don't know how much water to add. I only guessed. You don't want enough to settle anywhere. Just enough to soak into the mixture without looking too damp. If it's glistening and looks like you can squeeze a lot of water out, it's too wet. I started with 10 cc's, waited a min., 10 more and hit it again with another 20 cc's. It doesn't matter if you hit the same spot as the spores. Try not to hit the mycelium - it may stunt the growth.


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OfflineCrazyPsilocybe
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Re: Should Spores Be Visible in Syringe? [Re: CrazyPsilocybe]
    #5619471 - 05/11/06 11:13 PM (17 years, 8 months ago)

Oh yeah - I got a little off topic. I ordered mine from earthstongue.com and they were VERY visable - just look at the pic on their website. 20CC syringes for the standard price.


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Offlinesatori85
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Re: Should Spores Be Visible in Syringe? [Re: splifner180]
    #5619538 - 05/11/06 11:26 PM (17 years, 8 months ago)

Quote:

splifner180 said:
I've obtained two syringes from a reputable Shroomery vendor. The first batch did nothing, no idea why. The second batch just got knocked up last last night so there is no data.

Both syringes were 100% crystal-clear with nothing at all (visible) floating inside. Is that normal?

tia - splif






New material? i had same thing happen to me right when i switched from buying online organic rye to buying store bought "organic rye". Turned it out had fungacide type stuff in it, nothing grew.


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