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InvisibleMasFina
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Syringe is almost empty and my jars are looking grim
    #5617076 - 05/11/06 12:42 PM (17 years, 8 months ago)

I used the wheat berry substrate as suggested in the Psilocybin Mushroom Handbook (minus the calcium carbonate and calcium sulfate they recomend adding to the jars) but after PCing the jars were too dry looking. The ones I inocculated 5 days ago aren't showing any signs of growth. So, I was wondering if it would be a good idea to inject some sterilized water into my more recently inoculated jars to try and save them. I am thinking of using my last 2 ccs of spores for a liquid culture, those are easy right? I don't wanna order another syringe.


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OfflineOneSense
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5617092 - 05/11/06 12:47 PM (17 years, 8 months ago)

Goooood idea.

Don't give up on your current jars, 5 days isn't long really. In the mean time start an LC if you have enough room to fruit all your jars if say an LC developes AND your current jars end up colonizing.


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Stash of Myco-Photos

"If I give advise or mention anything on the topic of mushroom cultivation, it was read within the archives of www.shroomery.org. would like to say that I grow active mushrooms however it is illegal to grow such natural fungi.. therefore my participiation in this hobby doesn't go past the reading of articles and viewing of photos within this website." - OneSense


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Invisiblemonstermitch
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Re: Syringe is almost empty and my jars are looking grim [Re: OneSense]
    #5617114 - 05/11/06 12:54 PM (17 years, 8 months ago)

for sure go with lc. Liquid culture can be easy if done right and will ensure an endless supply of spores and culture and mushrooms if the process is maintained correctly.

5 days may turn into two weeks, it's happened before with me, just be patient


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5617194 - 05/11/06 01:16 PM (17 years, 8 months ago)

So I should add extra water then? Also, does rye grain have this problem of drying out during PCing?


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
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Invisiblemonstermitch
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5617272 - 05/11/06 01:38 PM (17 years, 8 months ago)

what lid set up are you using?
I think the book uses filter discs, plastic lids, and 1/2 hole or so.?.


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5617395 - 05/11/06 02:13 PM (17 years, 8 months ago)

I am using postal tyvek with metal lids.


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
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PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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Invisiblemonstermitch
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5617648 - 05/11/06 03:11 PM (17 years, 8 months ago)

there in lies your problem. Maybe. Be more specific please.

This is the lid setup I use for grain.

Tyvek on top of jar.
Metal lid on tyvek with two 1/4 holes drilled in it for limited air exchange.
Metal band tightly securing both lid and tyvek down.
2 coffer filters on top of jar held tight just under band with a rubber band.

agar's threads is where I found that one.

Is this what you mean? Or are you only using tyvek with a band?


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Offlinesplifner180
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5618002 - 05/11/06 04:42 PM (17 years, 8 months ago)

MasFina, could you be a bit more specific? When you say metal lids you mean the saucer-shaped things, right? Not the threaded rings?

Also, what size holes do you have and how many?

It sounds to me like you have a setup in this order; jar, metal lid, threaded ring, tyvek secured over top. Is this correct?

splif


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First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
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OfflineOatman2000
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Re: Syringe is almost empty and my jars are looking grim [Re: splifner180]
    #5618070 - 05/11/06 04:55 PM (17 years, 8 months ago)

everything sounds great the way you made your jars...

how are you prepairing your grain... what was your method for adding moisture to the grain?


--------------------
Spawning to COIR
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WBS QUART SPAWN JAR PREPERATION
----------------------------

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Offlinesplifner180
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Re: Syringe is almost empty and my jars are looking grim [Re: Oatman2000]
    #5618552 - 05/11/06 06:57 PM (17 years, 8 months ago)

For the first run -- the one that hasn't popped in 13 days -- I boiled water and removed pot from heat. Put in 5 lbs of WBS (Kaytee brand). Let that steep overnight, always making sure the water level stayed above the top of the seeds. The next day I drained, let them sit in the colander for maybe 10 minutes, shook and filled the jars.

The end-product after PCing appeared to be perfect; water halos around individual seeds with no pooling.

The only thing I did differently this time is that I rinsed the crap out of the WBS just before draining this time around, maybe about 6 rinses. The first time I did maybe 2-3.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Invisiblemonstermitch
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Re: Syringe is almost empty and my jars are looking grim [Re: splifner180]
    #5618622 - 05/11/06 07:13 PM (17 years, 8 months ago)

I soak my wbs in 5 gallon buckets and make screen lids for them

I tip them over and drain, then rinse like 6 times

Next I invert and let the seed drain for 6 hours

This gets me perfect water content, but maybe the 6 hour drain is overkill? If 10 minutes works perfect than I will re-consider the drain time and do a test run...

off for more wbs for a trial.


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OfflineOatman2000
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5618799 - 05/11/06 08:00 PM (17 years, 8 months ago)

Yeah, i like to rinse the crap out of my Wbs too...

Wintertime sux, because i use my shower (my water pressure sux balls)



This works!


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: Oatman2000]
    #5619330 - 05/11/06 10:36 PM (17 years, 8 months ago)

Sorry for the delayed response, I've been out all day. I do my lids like this: metal disk has a small syringe hole and 2 1/4" breather holes. I put on the metal disc, then the tyvek (with plenty overhang), then the screw on ring. after innoculating i cover the inoc hole with electric tape or scotch tape and then put 1 alcohol soaked coffee filter over the jar and secure it with a rubber band. I then put it in my TIT setup which has an internal temp of 83.5. I used my oven as a flow hood. I realize this is sketchy, so I just fashioned a ghetto glove box out of saran wrap and my pressure cooker box. I just shot up 2 liquid culture jars with my last 2 cc's of spores using the glove box. So if my wheat berry jars are screwed I'll still be in business.


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5619355 - 05/11/06 10:41 PM (17 years, 8 months ago)

Also, I boiled my grains for 10 minutes then let them soak for 12 hours. I rinsed them well before putting in jars. I started timing the PC after it reached 15psi and cooked for 60 mins. The grain was very moist when I put it in the jars, I don't know where that water went. I added a splash of vinegar to the water in the PC, cuz the manual said to. I hope this didn't affect the substrate. Thats everything. I think wheat berries just suck, but maybe I missed a detail somewhere.


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5619370 - 05/11/06 10:46 PM (17 years, 8 months ago)

possible detail missed:

if you release the pressure of the pressure cooker youself instead of letting it dissipate on it's own it can vaporize the moisture inside of the jars.

I personally don't like wheat berries either... I use wbs.


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5620169 - 05/12/06 04:55 AM (17 years, 8 months ago)

Perhaps this is it. In the PC directions, it says to let it spew steam for five minutes before putting my bobbler on (which snuffs most of the steamflow out). I did that. Should I just put the bobbler on sooner to prevent all that steam from escaping?


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5620170 - 05/12/06 04:56 AM (17 years, 8 months ago)

I did let it cool naturally though.


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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InvisibleFooMan
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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5620225 - 05/12/06 05:47 AM (17 years, 8 months ago)

Quote:

MasFina said:
Perhaps this is it. In the PC directions, it says to let it spew steam for five minutes before putting my bobbler on (which snuffs most of the steamflow out). I did that. Should I just put the bobbler on sooner to prevent all that steam from escaping?




The steam is from the water on the bottom of the PC, not the jars themselves. What you did was called "venting" and it gets rid of any pockets of air inside of the PC so that it works more effectively. You want to make sure you don't pull the weight off at the end and let the steam escape suddenly. That can dry and even crack your jars like that. The wheat berries should have worked. The only possibilities I can think of are that the spores were crap or the jars themselves weren't completely cool when you inoculated. Even when a jar feels cool on the outside, the internal temp may be high enough to kill the spores.


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: FooMan]
    #5621264 - 05/12/06 01:16 PM (17 years, 8 months ago)

Spores were from spores 101 and jars were definitely cool. I'm stumped


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
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PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5621387 - 05/12/06 02:04 PM (17 years, 8 months ago)

was there water still left in the bottom of the pc?


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5621813 - 05/12/06 04:33 PM (17 years, 8 months ago)

yes


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Syringe is almost empty and my jars are looking grim [Re: MasFina]
    #5621839 - 05/12/06 04:42 PM (17 years, 8 months ago)

stumped too, I believe.

dead rocket??


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InvisibleMasFina
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Re: Syringe is almost empty and my jars are looking grim [Re: monstermitch]
    #5622863 - 05/12/06 09:08 PM (17 years, 8 months ago)

I injected them (1 pint jars, cuz i only have an 8 quart PC) with 10cc's a piece of sterile water to try and rehydrate them. I'll let you know if they pull through.


--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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