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Offlinebajone
cccp

Registered: 11/03/05
Posts: 116
Last seen: 11 years, 18 days
myc colonization stoped!
    #5616351 - 05/11/06 06:52 AM (17 years, 8 months ago)

I did popcorn teq. 1kg of popcorn soaked, then cooked, and sterilized in autoclavable bag. It is one month since myc stoped growing and expanding. First week myc colonization progressed fine, then I shaked the bag to improve faster colonization, but it caused myc to stop expand on corn.
What should I do!?!?!?!


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Offlinebajone
cccp

Registered: 11/03/05
Posts: 116
Last seen: 11 years, 18 days
Re: myc colonization stoped! [Re: bajone]
    #5617323 - 05/11/06 01:51 PM (17 years, 8 months ago)

any suggestion?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: myc colonization stoped! [Re: bajone]
    #5617365 - 05/11/06 02:04 PM (17 years, 8 months ago)

Is the bag wet??

If not, leave it alone. Seriously just wait and see.

Is there a thin white coating on most of ther kernels?


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Offlinebajone
cccp

Registered: 11/03/05
Posts: 116
Last seen: 11 years, 18 days
Re: myc colonization stoped! [Re: monstermitch]
    #5631425 - 05/15/06 05:23 AM (17 years, 8 months ago)

No, a bag is dry. Only one part of corn colonized, and there is some white coating on them. But other 90% of corn isn't colonized. I warmed up the ambient to 27-28 C to see if this change the situation.
Thanx!


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Offlinejohnuk
Strangerlove
Registered: 06/13/05
Posts: 226
Last seen: 13 years, 8 months
Re: myc colonization stoped! [Re: bajone]
    #5631551 - 05/15/06 07:33 AM (17 years, 8 months ago)

I don't like shaking jars. Maybe it's just me, but I've only ever seen it do something similar to what you've mentioned. You shake them up and they tend to just stop.

I shook up a jar recently and the growth just disappeared. I thought it had died on me but after about a week I started to see a few grains refluffing with myc. There are pockets of 5 - 10 grains with myc on them.

On the other hand, a jar I haven't shaken is miles ahead and is about 70 - 80% colonised.

I've given up on shaking once the myc appears. It seems kind of wrong as well. The myc is incredibly fragile and will easy get messed up in the process of shaking. It might spread it around a bit more, but it's not doing the myc it's self any favours. I now prefer to just shake the ass out of the jars immediately after spore injection. You only get the irregular colonisation because the spores have clustered somewhere, so shaking them really, really well whilst it's only the spores present (which won't get damaged by doing so) makes more sense to me.

I can see why it might be more important if you were starting from a living myc but I'd be super gentle with that if I needed to redistribute it.

With spores though, I think from now on I'll inject ~0.2cm^3, shake, inject a bit more, shake and so on until I get to 1cm. Then I'll shake it for a minute solid and then leave it alone.


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OfflineLord_Shiva
The Mahadeva
Male

Registered: 05/15/06
Posts: 37
Last seen: 17 years, 1 month
Re: myc colonization stoped! [Re: johnuk]
    #5631842 - 05/15/06 09:59 AM (17 years, 8 months ago)

I can only agree to johnuk, for me shaking jars mostly turns out to be a bad idea. After shaking it looks like some parts of the grain recover, some don`t. So I have dead parts of mycelium within my jars, and the healthy myc needs a lot of time to overgrow these. If I don`t shake my jars, they colonize faster, and the different substrains have more space for themselves.


--------------------
satyam shivam sundaram


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