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Offlinechris999
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Is it Time!?!? (update)
    #5612824 - 05/10/06 01:51 PM (17 years, 8 months ago)

ok everyone update with my grow! Ok my ars were sterilized and pulled out of the water at about 4 and a half hours ago, i just arrived back at home to find them nice and cool (room temperature). I have put them in the fridge about 10 min ago in hopes that any heat still withjin the cake will cool i think i wil let sit for 15 more min. Do u think its time to inoculate them or should i leavfe them in the fridge a bit longer :thumbup: :mushroom2:??


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Edited by chris999 (05/10/06 02:51 PM)


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Offlinersd86
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Re: Is it Time!?!? [Re: chris999]
    #5612849 - 05/10/06 01:58 PM (17 years, 8 months ago)

Might be. I always like to err on the side of caution. But If you've had them in the fridge and your jars didn't crack, maybe.
Though, a few hours wait isn't really gonna set they're birthday back that much.


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"Lack of control for only one millisecond can cause you to piss on your toilet seat."


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Offlinechris999
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Re: Is it Time!?!? [Re: rsd86]
    #5612955 - 05/10/06 02:16 PM (17 years, 8 months ago)

how long do i hve to wait to inject the spores after i flame the needle??


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OfflineCrazyPsilocybe
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Re: Is it Time!?!? [Re: chris999]
    #5612999 - 05/10/06 02:25 PM (17 years, 8 months ago)

Not long. I wipe with alc. soaked paper towel to cool the syringe.


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"I hate to advocate drugs, alcohol, violence or insanity to anyone, but they've always worked for me."
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Offlinechris999
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Re: Is it Time!?!? [Re: CrazyPsilocybe]
    #5613068 - 05/10/06 02:39 PM (17 years, 8 months ago)

ok i just got done knocking up the jars, ok heres what i did to the syringe
i alcohol flamed it till red hot then colled with alcohol saoked t.p. and then stuk in hole and squirted. the after each hole i wiped down the needle with alcohol tp. so do u think that i am ok and i will get good results if everything is steril and how it should be?


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Offlinefrankthetank87
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Re: Is it Time!?!? [Re: chris999]
    #5613271 - 05/10/06 03:23 PM (17 years, 8 months ago)

chris, you might not even get growth at all. alcohol kills spores. If you shot spores into your jars, and then the alcohol from the needle touched them- they are as good as dead. Make sure you clean with water after alcohol before inoculation.


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-frank the tank-
Anything said above by the user frankthetank87 is completely fictional and used for entertainment purposes only.


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OfflineBlek
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Re: Is it Time!?!? [Re: frankthetank87]
    #5613303 - 05/10/06 03:30 PM (17 years, 8 months ago)

Sounds good chris. Did you put some sort of filter tape or a band aid over the inoculation holes after you squirted the spore solution in there?

Your inoculation process sounds good though. I don't even flame my needles anymore before innoculating, I just soak a rag or paper towel in alcohol and use that to wipe down the needle about 5 seconds before innoculating.

Also, shake up those jars after inoculation to spread the spore solution around evenly. This will give you the fastest colonization times.


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OfflineMustardMan
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Re: Is it Time!?!? [Re: frankthetank87]
    #5613306 - 05/10/06 03:31 PM (17 years, 8 months ago)

I've heard that its smart to let your jars sit for 1-2 days before inoculating, this way you can see if any bacteria has developed before waisting spores. Also, this way you will be absolutely sure that the jars are cool :smile:


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Invisiblemonstermitch
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Re: Is it Time!?!? [Re: Blek]
    #5613318 - 05/10/06 03:33 PM (17 years, 8 months ago)

I disagree with frank the tank. Using a alcohol rub will not kill your spores, if you clean with water after alcohol, you are defeating the purpose of alcohol. No real need to flame unless you suspect contam, just keep the needle in an alcohol soaked pad the whole time never letting the metal be exposed to the air.

Check out agar's open air inoculation tek.

You are fine, sit back and wait, then wait some more, it'll all come in due time.


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Offlinechris999
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Re: Is it Time!?!? [Re: monstermitch]
    #5613335 - 05/10/06 03:38 PM (17 years, 8 months ago)

good info, and btw no i dod not cover the holes at all because i hav read that most ppl do not cover there holes... Should i cover them ( ithought i should leave them open for FAE


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Offlinesplifner180
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Re: Is it Time!?!? [Re: frankthetank87]
    #5613367 - 05/10/06 03:43 PM (17 years, 8 months ago)

DEFINITELY cover those holes with micropore tape, IMMEDIATELY. The jars need to breathe.

splif


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First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
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Offlinechris999
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Re: Is it Time!?!? [Re: splifner180]
    #5613456 - 05/10/06 04:05 PM (17 years, 8 months ago)

i dont have that micro tape i have masking! what should do


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OfflineBPJohnny
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Re: Is it Time!?!? [Re: chris999]
    #5613529 - 05/10/06 04:20 PM (17 years, 8 months ago)

your doing PF with dry layer of verm correct? if so
no need to cover the holes your fine!

The whole reason for the dry layer of verm is to keep contams
out, and it can breath (somewhat) through the verm. if you cover up
those holes your not going to get much air in there and will
colonize slower.

On the other hand if your using grain (ie: WBS or rye) then microspore
is necessary, also if you did not use the dry verm layer.


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Offlinechris999
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Re: Is it Time!?!? [Re: BPJohnny]
    #5613538 - 05/10/06 04:22 PM (17 years, 8 months ago)

ohh ok good ignore my othr post then!


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OfflineBlek
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Re: Is it Time!?!? [Re: chris999]
    #5617139 - 05/11/06 01:01 PM (17 years, 8 months ago)

I didn't read your post carefully enough and didn't know you were doing PF style jars. But yeah, what Johnny said!


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