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johnuk
Strangerlove
Registered: 06/13/05
Posts: 226
Last seen: 13 years, 8 months
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Shaking up spore syringes
#5612631 - 05/10/06 12:56 PM (17 years, 8 months ago) |
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I made up two P. Mexican syringes about a week or two ago and have noticed that the spores tend to clump together in one big lump within a few days - usually on the lowest part of the rubber seal for some reason (I don't store them vertically).
I've tried shaking these things really violently, so much so that the blade few off one of the syringe a few times.
I gave my Phillip's Sonicare ultrasonic toothbrush a go. Not much luck whichever way I touched the syringe body with it.
The only way I managed to redistribute the clump was to stick the blade into a previously sterilised conical flask of LC and draw in some clean air. Then I could shake it up and break the clump free. Bit more shaking and I have lots of smaller clumps.
Do any of you have any kind of rituals for shaking up your syringes?
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rsd86
Redneck TruckDriver


Registered: 05/30/05
Posts: 39
Loc: THE SOUTH
Last seen: 2 years, 6 months
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Re: Shaking up spore syringes [Re: johnuk]
#5612635 - 05/10/06 12:59 PM (17 years, 8 months ago) |
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Nothin cept what you did. Just suck up a bit of air, recap and shake it like a british nanny.
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"Lack of control for only one millisecond can cause you to piss on your toilet seat."
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Shaking up spore syringes [Re: rsd86]
#5614030 - 05/10/06 06:18 PM (17 years, 8 months ago) |
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Tip: Use a two drops of JetDry per 100ml/cc water for your spore solution this will aid in the dispersal of these clumps. Now mind you the spores will still have a tendency to attract one another a slight tap will disperse them easily. As for the ones you have now simply try tapping themfirmly again the edge of the table several times rather than shaking them.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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