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OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 4 months, 6 days
Re: Cordyceps, stage one complete! [pics] [Re: Anno]
    #2407173 - 03/08/04 01:06 PM (19 years, 11 months ago)

Holy baloney, I've never seen that pic before. I'll have to delve into mushworld to get the specifics. WOW!!

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Anonymous

Re: Cordyceps, stage one complete! [pics] [Re: Suntzu]
    #2407353 - 03/08/04 01:45 PM (19 years, 11 months ago)

culture medi aprepared from rice, corn, rice husks, silhworm pupa powder, water , glucose, peptone, potassium dihydrogen phosphate , magnesium sulfate, triammonium citrate abd V b1. Was maybe used for that aoustanding flush. That was a recipe I found on Mushworld.

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InvisibleSpeeker

Registered: 02/11/04
Posts: 880
Re: Cordyceps, stage one complete! [pics] [Re: Suntzu]
    #3740595 - 02/06/05 12:33 PM (19 years, 27 days ago)

In this article is info about Cordyceps cultivation:
THE HYBRIDIZATION OF CORDYCEPS SINENSIS STRAINS

They use snake venom as a hybridization agent!

more hybridization/fusion related posts:
search results for query: fusion

:sun:

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InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: Cordyceps, stage one complete! [pics] [Re: Speeker]
    #3743202 - 02/06/05 10:43 PM (19 years, 26 days ago)

"The agar used for this hybridization is an Aloha Medicinals Inc. proprietary agar named R7 Agar, consisting of malt extract, activated carbon, minerals and humus ? the carbon-rich ash residue from a coal burning industrial process"

2.1 L Distilled Water
50 g Light Malt Extract
34 g Agar
10 g Humus
5 g Activated carbon
1 g MgSO4
10 ml 1% KOH solution


I am curious to know if cordyceps spores will germinate readily on agar or not. This article says nothing about it. They used "available" strains... Available from where? (without paying an arm and a leg for one)



I read that article awhile back researching cordyceps, and thought SNAKE VENOM?
Yeah, that should be easy to acquire... Risk my life to grow a mushroom...
I think not... I am no Terry Erwin...


--------------------
To give is to live...


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OfflineFood
---Beast---

Registered: 12/10/01
Posts: 390
Loc: Siberia
Last seen: 17 years, 10 months
Re: Cordyceps, stage one complete! [pics] [Re: ATWAR]
    #3808340 - 02/20/05 07:48 PM (19 years, 12 days ago)

So cool - that first picture of the cordyceps eating the bugs . Coool .


--------------------
--------mushworld.com-----More info than you can throw a stick at-

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Invisiblecricket
Lord Cricket
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Registered: 08/29/03
Posts: 961
Loc: in my house, in front of ...
Re: Cordyceps, stage one complete! [pics] [Re: Food]
    #3810200 - 02/21/05 09:15 AM (19 years, 12 days ago)

Snake venom is easier to obtain then you think. I have two spiecies of cobras, seven spiecies of rattlesnakes, and two african vipers in my basement right now.
Has anyone tried this with crickets? Or shed skins from crickets?
I had a fungal infection wipe out most of a colony of crickets. I did not try to ID it but it produced small pin shaped fuiting bodies on the carcasess.
I had a plastic trash can with wet gravel on the bottom. I used 2 inch pieces of pvc pipe, and cardboard disks stacked to make several levels. (like a highrise apatment) After a few months I noticed a Mushie smell and no crickets. As I unstacked the cardboard disks I found two layers covered with dead and dying adult crickets. The young crickets looked a little fuzzy but they were hatching and surviving untill they reached adult size. They must have had time to breed at least once because I had a constaint supply of babies showing up.
I'm not sure if it had anything to do with it but some of the boxes I used were from fruit boxes shipped from Asia.
I never had this problem with all American boxes.


--------------------
I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?

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Invisiblecricket
Lord Cricket
Male User Gallery

Registered: 08/29/03
Posts: 961
Loc: in my house, in front of ...
Re: Cordyceps, stage one complete! [pics] [Re: cricket]
    #3832840 - 02/25/05 11:58 AM (19 years, 8 days ago)

Anything?


--------------------
I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?

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InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: Cordyceps, stage one complete! [pics] [Re: cricket]
    #3841987 - 02/27/05 03:40 PM (19 years, 6 days ago)

The original culture that Suntzu had was found to be a mislabeled culture, and was most likely a mold of some sorts (I can't remember the specifics, and I can't post PM's). It appears there will be little development in this arena (on the Shroomery that is) until someone obtains a "real" culture. They appear to be difficult to come by, especially at a cheap price that an experimenter can afford...


I am still considering trying to obtain some intact specimens of cordyceps in dried form to collect spores from. The only problem I see is that the spores germinate on contact with the living host (or inside it?), then go on to fruit from its carcass. I have no idea if the spores would germinate on agar without some type of special preparation. I have done little research on this since the culture was found to be mislabeled. But, I am still very interested in this species...

To get it in culture is still my biggest hurdle...
At least one site I found has a picture of mycelium:

Quote:

Growth in culture (PCA) obtained from germinating ascospores or conidia; colony slow-growing, mycelium at first whitish, soon turning orange yellow, partly submerged in agar, after 3 to 4 weeks producing aerial mycelium including dense, radially arranged whitish to pale yellowish fascicles of hyphae, which produce verticillate hyaline phialidic conidiophores repeatedly, branches of conidiogenous cells aggregated into feather-like synnemata up to 15 mm long, phialides 4-6.5 x 2.3-3.3 mm, flask-shaped with a sharply narrowed, elongate neck, sympodially proliferating. Conidia narrowly cylindrical to fusiform, 3.2-5.0 x 0.8-1.0 mm.

http://www.uio.no/conferences/imc7/NFotm99/August99.htm









Care to collect some venom for me?
Without envenomating yourself?
:tongue:


--------------------
To give is to live...


Edited by ATWAR (02/27/05 09:17 PM)

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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Cordyceps, stage one complete! [pics] [Re: ATWAR]
    #3843900 - 02/27/05 08:54 PM (19 years, 5 days ago)

Why wouldnt the spores germinate? Its readily available nutrients. Give the mycelia what it needs as in nutrition and watch it grow. Think of the dish itself as the exoskeleton.

That doesnr look like any mycelia I have ever seen. If you germinate spores please keep one aside id love to check that out.

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InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: Cordyceps, stage one complete! [pics] [Re: Corporal Kielbasa]
    #3844044 - 02/27/05 09:16 PM (19 years, 5 days ago)

Quote:

SHEIKofSHIITAKE said:
Why wouldnt the spores germinate? Its readily available nutrients. Give the mycelia what it needs as in nutrition and watch it grow.





With some species it is not always that simple.
Take the chanterelle for example.
In this case I hope it is...


--------------------
To give is to live...


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InvisibleSpeeker

Registered: 02/11/04
Posts: 880
Re: Cordyceps, stage one complete! [pics] [Re: ATWAR]
    #3845095 - 02/28/05 12:34 AM (19 years, 5 days ago)

Maybe this oldie helps? (meat extract agar)
http://www.shroomery.org/forums/showflat.php/Number/420355#Post420355

www.mycoculture.org seems to be down..  :frown:
some nice post there was....

Fusarium it was..
ATTN; anyone who has received Cordyceps cultures
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3157089/an//page//vc/1


:sun:

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Invisiblekorins
Antisocialite

Registered: 02/21/05
Posts: 221
Re: Cordyceps, stage one complete! [pics] [Re: Suntzu]
    #3848401 - 02/28/05 06:23 PM (19 years, 4 days ago)

Did you ever make any more progress Suntzu?

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OfflineSuntzu
Geek
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Registered: 10/14/99
Posts: 1,396
Last seen: 4 months, 6 days
Re: Cordyceps, stage one complete! [pics] [Re: korins]
    #3882157 - 03/07/05 10:48 AM (18 years, 11 months ago)

Not without a real Cordyceps! I tried swabbing some puchased cordyceps fruitbodies onto agar without success. Someone gave me a link to the 'real deal', but this guy wanted WAY too much money for a culture [and how do you trust it anyway??].

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Offlinekenndeb
Stranger
Registered: 06/03/08
Posts: 15
Last seen: 15 years, 4 months
Re: Cordyceps, stage one complete! [pics] [Re: Suntzu]
    #8483247 - 06/04/08 11:20 AM (15 years, 8 months ago)

when and if I ever get a pure culture of cordyceps going, I was planing on using grubs collected from under logs in the woods or out of the lawn...perhaps growing the culture in a milo/soil media with the grubs being introduced shortly after inoculation...

as far as snake venom..there are several biochem companies that sell it..I just got some in last week...

one last note...I posted a new thread(perhaps I should just have posted here) Cordyceps militaris cultivation
#8481790 - 06/03/08 11:27 PM
I could really use some input...thanks

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Offlinebioasi
Stranger
Registered: 11/23/08
Posts: 1
Last seen: 13 years, 4 months
Re: Cordyceps, stage one complete! [pics] [Re: Suntzu]
    #10487002 - 06/11/09 08:24 AM (14 years, 8 months ago)

Please can you help me to find the culture of cordyceps and try to grow them ,please it is all most impossible to find the culture, i hope you share the fun of it.please

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Offlinejjb007

Registered: 10/03/08
Posts: 338
Loc: Near Philly
Last seen: 1 year, 3 months
Re: Cordyceps, stage one complete! [pics] [Re: bioasi]
    #10487498 - 06/11/09 10:55 AM (14 years, 8 months ago)

Quote:

bioasi said:
Please can you help me to find the culture of cordyceps and try to grow them ,please it is all most impossible to find the culture, i hope you share the fun of it.please




This post is 7 years old so you may not get many replies...
You may want to try the marketplace forum (which you don't get access to until your a member for 3 months or have a certain amount of posts, I dont recall which it is)

Or post your own post in the gourmet forum...

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Offlinevonswarrior
Vonswarrior
Male

Registered: 06/11/06
Posts: 69
Last seen: 10 years, 11 months
Re: Cordyceps, stage one complete! [pics] [Re: bioasi]
    #10487633 - 06/11/09 11:34 AM (14 years, 8 months ago)

bioasi

I would suggest try to grow something easier like oyster mushrooms, if you are a beginner, growing Cordyceps ain't easy to grow even for the most experienced,I have not seen anyone here that ever had success growing cordyceps, there will be a few members if you ask them that will share you a oyster wedge .

Henry

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Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


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