Quote:

---

Ziplok said:
I've got some atlantis jars at 14 days with dime sized sclerotia forming in all of them. I used finch seed.

---

Quote:

---

Juke Adro said:
I found normal rye grain NOT seed to perform better.
Wild Bird seed is also good.
grass seed compacts too much.

---

Quote:

---

Cyberdog said:
It was a lot of guess work but mostly I never add enough moisture to crack the hull and expose the starch. So, this tek has me sceptical.
they just mentioned using a one-for-one ratio of rice to water if I do this.
Is it going to make the rice sticky?

---

Quote:

---

Cyberdog said:
Also does the necessity change for shaking if you're using multi-spore inoculation or live culture?

---

Quote:

---

blackout said:

To improve shakeability I have added popcorn to my rice jars. They are so hard its like ballbearings you get in cans of spraypaint, it breaks up the rice really well.

---

Quote:

---

RogerRabbit said:
Nice idea!  Thanks for sharing.

---

thanks, great to see you back around again!

Quote:

---

RogerRabbit said:
I assume you're not fully hydrating the corn first so it stays hard?

---

Quote:

---

blackout said:

Quote:

---

Cyberdog said:
It was a lot of guess work but mostly I never add enough moisture to crack the hull and expose the starch. So, this tek has me sceptical.
they just mentioned using a one-for-one ratio of rice to water if I do this.
Is it going to make the rice sticky?

---

Do you know how much water you added before? If soaking or cooking you can weigh rice before and after to figure out how much you added. Some people dry out grains to figure out the exact moisture content, rather than added moisture, but this is usually not needed.

The 1 to 1 ratio is presumably volumentric. This is quite low already. The 9er tek calls for 1.5 volume water to 1 volume rice.

The last rice I used for sclerotia was 180g(~180ml) water to 100g rice. Googling shows 1L of rice is 782g, so 100g is about 128ml, so my volumetric ratio was approx 1.4:1 water:rice. My rice was fairly shakeable at that.

---

HAHA! so this is what happend:


blackout, like I said that had me a little sceptical. I have a very accurate scale. well this is exactly 180g water and 100g organic brown rice thrown into the PC for 90 min. Is this gelatinous cake what I want to grow sclerotia in?
please correct me.

I seriously want to get the whole "one and done" method down(is that a thing?) where you measure out exactly how much moisture you want and then cook.

Quote:

---

waixingren said:
9er tek basicly. http://www.shroomery.org/index.php/par/7923/pag/3

cook like you're gonna eat it then pc.

---

Quote:

---

EXPERIMENTAL:

The strain of Psilocybe mexicana was obtained from the "underground mushroom movement" of the U.S.A. A successful fruiting experiment on rye grass seed (Lolium sp.) water mixture by using a casing layer (POLLOCK, 1977; STAMETS & CHILTON, 1983) and the subsequent microscopic examination of the few fruit bodies (GUZMAN, 1983) confirmed the identity of the species. Mycelium was kept as a stock culture on 4% malt agar.

Cotton - plugged 500 ml Erlenmeyer flasks were filled with 100 g grain and 180 ml water, sterlised by autoclaving, cooled, inoculated from stock cultures and incubated at 23 C in darkness to promote the formation of sclerotia. Rye grain, sfot rice grain and rye grass seed were used, respectively. Fruit bodies of Psilocybe natalensis (leg. O'Neill's cottage, Natal-South Africa, Jan. 22, 1994) were dried at 20-40 C.

Possible present residual water was removed from the mushrooms by freeze drying. Mycelium obtained from spore prints (STAMETS & CHILTON, 1983) was also kept as a stock culture on 4% malt agar. The mycelium from a cultivation on 100 ml agar was analysed after 4 weeks of cultivation.

Mycelium from Psilocybe azurescens (STAMETS & GARTZ, 1995) on 4% malt agar was used to inoculate a rye grain/water mixture identically to the cultivation of Psilocybe mexicana. After 3 weeks cultivation 500 g of sawdust soaked with water in plastic bag was inoculated with the mycelia on grain (STAMETS & CHILTON, 1983). The duration of the spawn run was 4 weeks.

In March commercial garden mulch in a shady outdoor bed was spawned with the mycelia on sawdust. A weekly water to keep the moisture content high. In September of the same year about 200 mushrooms appeared. Some were dried for anaylsis in the same way as Psilocybe natalensis.

Dried fruit bodies of Galerina steglichii from (BESL, 1993) were also analysed. It was possible to isolate a strain from spores on 6% malt agar. The extraction procedures of mushrooms and mycelia as well as the analysis by using HPLC and TLC are described elsewhere (GARTZ, 1989, 1991b).

---

Quote:

---

Uninoculated Philospher's Bag - Consistancy is like modeling clay and appears to consist mostly of rice. Syringe passed through the injection port and easily into the substrate. When the inoculate was added it flowed out of the substrate and into the space between the substrate block and the bag. I used a full 10 cc syringe since I had it available and I wanted to speed things up. A few spots of growth on the surface of the substrate were visible in 4 days at 85F and quickly spread. It reminds me of mycelium growing on the surface of an agar plate.

---