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 ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
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 think washing your hands is sufficient ? 
#5607617 - 05/09/06 05:22 AM (17 years, 11 months ago) |
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well, it itsn't.
after thoroughly washing my hands i decided to print two of my fingers
on a sterile agar plate, just to see how clean they actually were.
well, see for yourself.
 
it proved to be essential to additionally wash your hands with something like rubbing alcohol
or alike in order to furthermore decrease number of still living contam sources.
just thought i'd share, for all the visual learners 
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 MONOTUB tek  HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Holydiver
Stranger
Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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 Re: think washing your hands is sufficient ? [Re: ohmatic]
#5607650 - 05/09/06 05:52 AM (17 years, 11 months ago) |
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Yep, just goes to show that washing ungloved hands is pointless, and anti-bacterial soaps are a haox (this has been proven). The only way to clean hands is to use latex gloves and clean those thoroughly w/ alcohol.
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To find a place to live between the negatives and positives.
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 sunit
Big Nose
Registered: 11/04/05
Posts: 224
Last seen: 14 years, 3 months
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Re: think washing your hands is sufficient ? [Re: Holydiver]
#5607669 - 05/09/06 06:11 AM (17 years, 11 months ago) |
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good advise
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Well, I set my monkey on the log
And ordered him to do the Dog
He wagged his tail and shook his head
And he went and did the Cat instead
He's a weird monkey, very funky.
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nugjug
Wanderer
Registered: 11/07/05
Posts: 703
Last seen: 6 years, 2 months
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Re: think washing your hands is sufficient ? [Re: sunit]
#5607701 - 05/09/06 06:58 AM (17 years, 11 months ago) |
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I did not know that. The second casing I did I said screw it because I couldn't figure out where I'd put my gloves. Outcome: contaminated. Apreciate the info.
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darkice
Stranger
Registered: 03/31/05
Posts: 48
Last seen: 15 years, 7 months
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Re: think washing your hands is sufficient ? [Re: nugjug]
#5607919 - 05/09/06 09:15 AM (17 years, 11 months ago) |
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I remember from labschool that washing even releases more bacteria because you scrub them out of your pores. A plate with unwashed hands was generally cleaner.
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: think washing your hands is sufficient ? [Re: Holydiver]
#5607937 - 05/09/06 09:23 AM (17 years, 11 months ago) |
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Quote:
Holydiver said:
Yep, just goes to show that washing ungloved hands is pointless, and anti-bacterial soaps are a haox (this has been proven). The only way to clean hands is to use latex gloves and clean those thoroughly w/ alcohol.
Stamets cleans his bare hands with iso and does agar work  .
He says he hates how gloves restrict his fingers lol.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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onetime
onetime
Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 4 months
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Re: think washing your hands is sufficient ? [Re: Psychoslut]
#5607965 - 05/09/06 09:33 AM (17 years, 11 months ago) |
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ever notice how surgeins wash their hands. They spend like 25minutes scrubbing at them
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See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want
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Drone
Derka Derka Derka
Registered: 07/22/04
Posts: 667
Loc: Maryland
Last seen: 5 years, 3 months
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Re: think washing your hands is sufficient ? [Re: onetime]
#5608292 - 05/09/06 11:13 AM (17 years, 11 months ago) |
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what about using instant hand sanitizer
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: think washing your hands is sufficient ? [Re: onetime]
#5608300 - 05/09/06 11:16 AM (17 years, 11 months ago) |
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Quote:
onetime said:
ever notice how surgeins wash their hands. They spend like 25minutes scrubbing at them
Drenching your hands in alcohol and cleaning under your fingernails with a brush is plenty clean enough IME.
Instant hand sanitizer is gelled alcohol so it does work good.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: think washing your hands is sufficient ? [Re: Psychoslut]
#5608332 - 05/09/06 11:25 AM (17 years, 11 months ago) |
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I use alcohol washed latex gloves and tyvek wrist sleeves. Never work bare handed, no matter how much you wash.
I see aspergillis at 8 and 10 o'clock and cladosporium at 2 and 3 o'clock. Also, at least two different bacteria. The clear one is common. I wonder what the yellow bacterium is?
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: think washing your hands is sufficient ? [Re: RogerRabbit]
#5608353 - 05/09/06 11:31 AM (17 years, 11 months ago) |
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i don't work bare handed cause i use a glove box and i wash the gloves with alcohol. I only use a glove box for G2G and syringe making. Everything else like inoculating and all that I do in open air with bare hands.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: think washing your hands is sufficient ? [Re: Psychoslut]
#5608357 - 05/09/06 11:32 AM (17 years, 11 months ago) |
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Doing any agar work without gloves is simply foolish and irresponsible as well as very unprofessional with that said washing your hands as well as forearms and spending the extra time to scrub well under your cut and trimmed nails should always be done whether or not you are going to where gloves. There are very viable reasons why surgeons scrub as well as wear gloves but ya know the world is full of hacks we see them everyday. I have done alot of agar work both ways with great results but I also have a very efficient sterile culture technique which aids in my success others may not be so fortunate. I will be the first to admit I am sometimes foolish as well as a little irresponsible but now days gloves are a must!!! Just try to get a tattoo without the artist wearing gloves and I'll show you one place no one should ever go to get a tat!
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: think washing your hands is sufficient ? [Re: RogerRabbit]
#5608360 - 05/09/06 11:34 AM (17 years, 11 months ago) |
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Quote:
RogerRabbit said:
I use alcohol washed latex gloves and tyvek wrist sleeves. Never work bare handed, no matter how much you wash.
I see aspergillis at 8 and 10 o'clock and cladosporium at 2 and 3 o'clock. Also, at least two different bacteria. The clear one is common. I wonder what the yellow bacterium is?
RR
I wouldn't expect any less from you bro!  BTW the yellow is more than likely yeast.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 4 days, 13 hours
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Re: think washing your hands is sufficient ? [Re: hyphae]
#5608369 - 05/09/06 11:37 AM (17 years, 11 months ago) |
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I have a confession to make: I have yet to use latex gloves in anything mycology related.
I tend not to touch anything that could contaminate with my bare hands though....
Edited by Anno (05/09/06 11:41 AM)
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: think washing your hands is sufficient ? [Re: Anno]
#5608456 - 05/09/06 12:04 PM (17 years, 11 months ago) |
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Quote:
Anno said:
I have a confession to make: I have yet to use latex gloves in anything mycology related.
I tend not to touch anything that could contaminate with my bare hands though....
Good thing is you don't have to worry about them coming back and suing your ass!!! LOL  
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: think washing your hands is sufficient ? [Re: hyphae]
#5608477 - 05/09/06 12:09 PM (17 years, 11 months ago) |
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I have done alot of agar work both ways with great results but I also have a very efficient sterile culture technique
Finding your own technique that works best for you is the most important thing in this hobby.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: think washing your hands is sufficient ? [Re: Anno]
#5608537 - 05/09/06 12:25 PM (17 years, 11 months ago) |
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Quote:
Anno said:
I have a confession to make: I have yet to use latex gloves in anything mycology related.
I tend not to touch anything that could contaminate with my bare hands though....
I've done both...bare handed and latex gloves.
and like Anno, I was very careful not to touch anything bare handed that would contaminate.
tc
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: think washing your hands is sufficient ? [Re: Anno]
#5608556 - 05/09/06 12:28 PM (17 years, 11 months ago) |
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Quote:
Anno said:
I have a confession to make: I have yet to use latex gloves in anything mycology related.
same goes for me, proper wash and rub with alcohol - i really dont like the way the gloves feel and hinder my movement.
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MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Cham
harro.
Registered: 04/02/06
Posts: 211
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Re: think washing your hands is sufficient ? [Re: ohmatic]
#5608565 - 05/09/06 12:30 PM (17 years, 11 months ago) |
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I'm a fan of having someone else lysol my gloves. Seems to do the trick.
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Cows would live a lot longer if they weren't made out of steaks and leather.
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Darkenshroom
PsychedelicExplorationist
Registered: 02/24/06
Posts: 683
Loc: I don't exist on this pla...
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Re: think washing your hands is sufficient ? [Re: ohmatic]
#5608572 - 05/09/06 12:32 PM (17 years, 11 months ago) |
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I wear gloves sometimes and not sometimes. When casing I tend to alcohol my hands and immediately after laying it in spray it with 20% h202/80% h20 mix, I also tend to lay very thin minute casing layers, thinner than most would suggest, but it gets me the type of pinsets I desire, every time I have laid an appropriately thick casing layer its either pinned horridly or contamed Thankfully contamed casing layer is relatively easy to deal with being contgained to the casing layer and all.
Darken
*smiles*
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~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~
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