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OfflineMustardMan
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Registered: 10/18/05
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Any tips for being sterile while dunking?
    #5601387 - 05/07/06 03:14 PM (17 years, 8 months ago)

Can I just dunk in bottled water? Where should I have it being dunked for 24 hours? When I roll the cake in dry verm, does that verm have to be sterilized some how?

Thanks.


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Offlinejoker_66599
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Re: Any tips for being sterile while dunking? [Re: MustardMan]
    #5601503 - 05/07/06 03:51 PM (17 years, 8 months ago)

naw well not from what i read. i have dunked before and just rolld the cakes on dry verm right from the bag.

but i can be wrong.


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I don't really grow mushrooms I just act like it to be coo.
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OfflineRogerRabbitM
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Re: Any tips for being sterile while dunking? [Re: joker_66599]
    #5601574 - 05/07/06 04:17 PM (17 years, 8 months ago)

I would put the dry verm on a cookie sheet and bake for half an hour at 300 or so just to be safe. If the bag has been open in your house, just about anything can be lurking there.

I dunk in tap water. If your local water is bad, use bottled or distilled.

Rinse the substrate under a faucet prior to beginning the dunk, then rinse again afterward. It's also best to dunk in a sealed jar in the refrigerator to prevent bacteria growing while the block is underwater.

If there's a few small pins on the cake or other substrate, don't pick them unless they're aborts. Healthy primordia/pins easily survive a dunk.
RR


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Offlineskeletor
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Registered: 09/06/05
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Re: Any tips for being sterile while dunking? [Re: RogerRabbit]
    #5601606 - 05/07/06 04:26 PM (17 years, 8 months ago)

ive just nuked teh moist verm for a few mins. only because it doens't take any time or else i wouldnt' bother doing anythign to the verm. even open bagged verm shouldn't have anything lerking in it. sure some spores migh ahve fallen on the top but when i sterilized the verm and let it cool more spores should fall on it again AS i put it out to rool in and to use for double end casing so whatever.


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OfflinedeXtrous
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Registered: 04/24/06
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Loc: Australia
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Re: Any tips for being sterile while dunking? [Re: skeletor]
    #5603774 - 05/08/06 02:06 AM (17 years, 8 months ago)

deleted by user ^^


Edited by deXtrous (05/08/06 02:13 AM)


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