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Offlinemeatloaf
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Registered: 05/06/06
Posts: 80
Last seen: 3 years, 7 months
some kind of contamination?
    #5598175 - 05/06/06 04:48 PM (17 years, 8 months ago)

i had a few pf jars going here they are.






all i want to know is, if they where contaminated or not and with what. thanks to anyone who can help and if you need anymore info just repost.


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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: some kind of contamination? [Re: meatloaf]
    #5599140 - 05/06/06 09:59 PM (17 years, 8 months ago)

cobweb mold. look it up


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First Grow No more carpet tek


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Offlinenoobles
Stranger
Registered: 02/24/06
Posts: 2
Last seen: 17 years, 8 months
Re: some kind of contamination? [Re: JSshroom]
    #5605284 - 05/08/06 02:58 PM (17 years, 8 months ago)

ok thanks alot, I did look at almost every contam it just did'nt look like it.


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InvisibleCureCat
Strangest
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Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
Re: some kind of contamination? [Re: noobles]
    #5607402 - 05/09/06 02:28 AM (17 years, 8 months ago)

Yes, i agree. It appears to be cobweb.


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: some kind of contamination? [Re: CureCat]
    #5607406 - 05/09/06 02:30 AM (17 years, 8 months ago)

How did they get contamed? did u not pc them?


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlinemeatloaf
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Last seen: 3 years, 7 months
Re: some kind of contamination? [Re: mikeownow]
    #5607490 - 05/09/06 03:13 AM (17 years, 8 months ago)

No i didn't PC them. i used the method of a pot and i put some hold pajama bottoms on the bottom of the pan. I also had them somewhat open in the incubation chamber, and I also opened them up in some fresh outside air. Could any of them other things have caused it?


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Offlinemeatloaf
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Re: some kind of contamination? [Re: meatloaf]
    #5607498 - 05/09/06 03:16 AM (17 years, 8 months ago)

I now have 2 more pf jars going i did PC them this time. Hopefully they don't get contaminated. there only 2 days from inoc now. maybe these will turn out better and not get contaminated.


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InvisibleCureCat
Strangest
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Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
Re: some kind of contamination? [Re: meatloaf]
    #5608309 - 05/09/06 11:19 AM (17 years, 8 months ago)

"No i didn't PC them. i used the method of a pot and i put some hold pajama bottoms on the bottom of the pan. I also had them somewhat open in the incubation chamber, and I also opened them up in some fresh outside air. Could any of them other things have caused it?"

Yup, all of those things can cause contamination. Even pressure cooking can fail to fully sterilize, so you are risking it much more with only a pot. Opening the jars AFTER sterilization is a BAD idea. So don't do it again! :-P


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Offlinenoobles
Stranger
Registered: 02/24/06
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Re: some kind of contamination? [Re: CureCat]
    #5611286 - 05/10/06 12:57 AM (17 years, 8 months ago)

OK right now i have the same jars and i put the little lid upside down and screwed the other cap on and it has holes poked for the inoculation with band aids covering the holes. is that bad?


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Offlinemeatloaf
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Registered: 05/06/06
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Last seen: 3 years, 7 months
Re: some kind of contamination? [Re: noobles]
    #5612673 - 05/10/06 01:12 PM (17 years, 8 months ago)

That was me above I have no clue whos account i was logged in but it sure wasn't mine.


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InvisibleCureCat
Strangest
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Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
Re: some kind of contamination? [Re: meatloaf]
    #5621001 - 05/12/06 11:57 AM (17 years, 8 months ago)

Huh, that is really weird.  I have heard various methods of placing the lid onto the jars.... I only took note and employed the one that seemed least susceptible to contamination.  I do not know what the need for turing the rubber seal lid upside down is, but i deff prefer to close it as it is meant.  I have had minimal contam probs thus far, one jar out of... 16.  Not bad.  So if you decide to flp them over, make sure EVERYTHING IS STERILE,  I cannot stress this enough, I wear a face mask so that i can get close to my work and be most precise and avoidant of possible contams withought barfing germs everywhere  :smile:  I use bleach and alcohol to sterilize, mostly alcohol (isopropyl 70% not 90%), use a lighter and cotton swab with alcohol to sterilize anything that is going to be close to or going into the jar (the needle of the syringe, and if you use scissors to cut up the bands aids- i use band aids as well, but I cut around the cotton part because i don't trust it's permeability and seems like if it became wet, it could light the whick of the candle so to speak).

Anyways, good luck to you  :smile:


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