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OfflineFGL
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Posts: 572
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Re: has somebody have success pasteurizing grains? [Re: skeletor]
    #5604163 - 05/08/06 07:46 AM (17 years, 8 months ago)

Quote:

skeletor said:
how do you plan to take 55g out and inject it and keep in sterile?




something similar to this:

http://www.shroomery.org/forums/showflat...rue#Post4355760


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OfflineFGL
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Posts: 572
Last seen: 9 years, 9 months
Re: has somebody have success pasteurizing grains? [Re: FungusMan]
    #5604169 - 05/08/06 07:48 AM (17 years, 8 months ago)

Quote:

FungusMan said:
Quote:

FGL said:
Quote:

FungusMan said:
I did many runs using fractional sterilization. I would soak and rinse the WBS as usual, then I would boil the jars 1 hour a day, for three days and innoc. I would also shake between boilings. Never had one contam. The only thing you have to watch for is moisture control. Add a little verm to help keep your jars from drying out. Never had any real problems doing it that way.




That is what I want to hear FungusMan  :thumbup: :thumbup: :thumbup:

do you think would be any problem if I do 4 runs instead of 3 and I want to do it in a 55g drum so I think it will take more time for getting the right temp in the whole substrate so maybe doing 3 to 4 hours runs would be better... or not? :rolleyes:

any opinion would be greatly appreciated  :grin: :mushroom2: :mushroom2: :mushroom2:




As long as you can keep the water boiling, 1 hour runs should be fine. The longer the runs, the more chance of grains splitting and turning to mush.Also let me add that, when boiling my jars, the jars were only HALF submersed. So, I really don't know how to apply this to drums, unless you stacked the jars in the barrel, and steamed them, in which I would do 4 runs of 1.5 hours, shaking each before each steam session.




I'll take your advice!!! :thumbup:
Many thanks for your help! :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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InvisibleFooMan
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5608152 - 05/09/06 10:27 AM (17 years, 8 months ago)

Quote:

FGL said:
thanks Tippinthru  :thumbup:

and the same question, has sombody have success with tyndallization? :grin:




Wow, I post the link for you almost right away and she gets the thanks :rolleyes: :smirk:


--------------------

Quick WBS Prep


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OfflineFGL
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Posts: 572
Last seen: 9 years, 9 months
Re: has somebody have success pasteurizing grains? [Re: FooMan]
    #5609523 - 05/09/06 05:03 PM (17 years, 8 months ago)

Quote:

FooManShroom said:
Quote:

FGL said:
thanks Tippinthru  :thumbup:

and the same question, has sombody have success with tyndallization? :grin:




Wow, I post the link for you almost right away and she gets the thanks :rolleyes: :smirk:




:sorry: FooManShroom, and many thanks for all the times you have helped me :thumbup: :thumbup: :thumbup:

and  :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: for your simmering no soakinng tek  :grin:


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: FungusMan]
    #5621797 - 05/12/06 04:21 PM (17 years, 8 months ago)

Quote:

FungusMan said:
Quote:

FGL said:
Quote:

FungusMan said:
I did many runs using fractional sterilization. I would soak and rinse the WBS as usual, then I would boil the jars 1 hour a day, for three days and innoc. I would also shake between boilings. Never had one contam. The only thing you have to watch for is moisture control. Add a little verm to help keep your jars from drying out. Never had any real problems doing it that way.




That is what I want to hear FungusMan  :thumbup: :thumbup: :thumbup:

do you think would be any problem if I do 4 runs instead of 3 and I want to do it in a 55g drum so I think it will take more time for getting the right temp in the whole substrate so maybe doing 3 to 4 hours runs would be better... or not? :rolleyes:

any opinion would be greatly appreciated  :grin: :mushroom2: :mushroom2: :mushroom2:




As long as you can keep the water boiling, 1 hour runs should be fine. The longer the runs, the more chance of grains splitting and turning to mush.Also let me add that, when boiling my jars, the jars were only HALF submersed. So, I really don't know how to apply this to drums, unless you stacked the jars in the barrel, and steamed them, in which I would do 4 runs of 1.5 hours, shaking each before each steam session.




would you add tape ( to prevent moisture entering the bags) to the filter if using filter patch bags instead of jars???


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InvisiblePsychoslut
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: FGL]
    #5621898 - 05/12/06 05:11 PM (17 years, 8 months ago)

Page 153 GGMM third edition

In devoloping countries, the sterilizer is ofter a makeshift, vertical drum heated by fire or gas. A heavy lid is placed on top to keep the contents contained. The boiling of water generates steam, which over many hours will sufficiently "sterilize" the substrate.


Who knows how long many hours is? Im guessing at least 5 :shrug:


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Psychoslut]
    #5621910 - 05/12/06 05:15 PM (17 years, 8 months ago)

No.

Do not tape up the filters on a filter patch bag.

No need to be blowing up fp bags and what not.


--------------------



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InvisiblePsychoslut
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: monstermitch]
    #5621959 - 05/12/06 05:31 PM (17 years, 8 months ago)

Taping over the patches on a fp bag would defeat the purpose. Who said that?


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Psychoslut]
    #5621963 - 05/12/06 05:32 PM (17 years, 8 months ago)

FGL

sorry Psychoslut, I mess up all the time by doing quick reply's to the last post.
I was talking to FGL.

looking to blow up bags... geeeesh.


--------------------



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OfflineFGL
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Psychoslut]
    #5621986 - 05/12/06 05:40 PM (17 years, 8 months ago)

Quote:

Psychoslut said:
Page 153 GGMM third edition

In devoloping countries, the sterilizer is ofter a makeshift, vertical drum heated by fire or gas. A heavy lid is placed on top to keep the contents contained. The boiling of water generates steam, which over many hours will sufficiently "sterilize" the substrate.


Who knows how long many hours is? Im guessing at least 5 :shrug:




here micolo2 have done it in 3 hours  :shocked:, but 5 hours should be safer... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5595140/page/0/fpart/1/vc/1

do you think  you kill the endospores without pressure in just one 5 hours run???


Edited by FGL (05/12/06 05:41 PM)


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OfflineFGL
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: monstermitch]
    #5622009 - 05/12/06 05:46 PM (17 years, 8 months ago)

Quote:

monstermitch said:
No. 

Do not tape up the filters on a filter patch bag.

No need to be blowing up fp bags and what not.




got it :thumbup:


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InvisiblePsychoslut
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: FGL]
    #5622032 - 05/12/06 05:55 PM (17 years, 8 months ago)

Quote:

FGL said:
Quote:

Psychoslut said:
Page 153 GGMM third edition

In devoloping countries, the sterilizer is ofter a makeshift, vertical drum heated by fire or gas. A heavy lid is placed on top to keep the contents contained. The boiling of water generates steam, which over many hours will sufficiently "sterilize" the substrate.


Who knows how long many hours is? Im guessing at least 5 :shrug:




here micolo2 have done it in 3 hours  :shocked:, but 5 hours should be safer... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5595140/page/0/fpart/1/vc/1

do you think  you kill the endospores without pressure in just one 5 hours run???




Im not exactly sure. I wish good ole Paul would of specified how many "many hours" is. Try 4 or five hours of steam and see what happens.

I imagine it has to be hot dense steam though. Make sure to put something heavy on the lid to hold in as much pressure as possible. Make it so the steam has to force itself through the tiny cracks between the pot and the lid.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


Edited by Psychoslut (05/12/06 05:57 PM)


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OfflineFGL
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Psychoslut]
    #5622104 - 05/12/06 06:14 PM (17 years, 8 months ago)

Quote:

Psychoslut said:
Quote:

FGL said:
Quote:

Psychoslut said:
Page 153 GGMM third edition

In devoloping countries, the sterilizer is ofter a makeshift, vertical drum heated by fire or gas. A heavy lid is placed on top to keep the contents contained. The boiling of water generates steam, which over many hours will sufficiently "sterilize" the substrate.


Who knows how long many hours is? Im guessing at least 5 :shrug:




here micolo2 have done it in 3 hours  :shocked:, but 5 hours should be safer... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5595140/page/0/fpart/1/vc/1

do you think  you kill the endospores without pressure in just one 5 hours run???






I imagine it has to be hot dense steam though. Make sure to put something heavy on the lid to hold in as much pressure as possible. Make it so the steam has to force itself through the tiny cracks between the pot and the lid. 




do you think it is safe? :rolleyes:


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InvisiblePsychoslut
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: FGL]
    #5622232 - 05/12/06 06:46 PM (17 years, 8 months ago)

yes it will be safe. you aint going to make the lid seal tight enough to blow up the pot. just put a brick or rock on the lid to hold in a little extra steam and give it a go.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineFGL
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Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Psychoslut]
    #5622314 - 05/12/06 07:06 PM (17 years, 8 months ago)

Quote:

Psychoslut said:
yes it will be safe. you aint going to make the lid seal tight enough to blow up the pot. just put a brick or rock on the lid to hold in a little extra steam and give it a go.




I'll post my results :cool: :thumbup:


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InvisibleHippie3
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: FGL]
    #5624469 - 05/13/06 12:19 PM (17 years, 8 months ago)

Quote:


for popcorn ...
Soak in warm water for 24 hrs
drain and rinse
bring to a boil then reduce to simmer until popcorn can be squished between fingers
drain and rinse with cold water
allow to drain for 4 hrs
load 1/2 quart jars with standard poly-filter and single inoculation hole
cover with aluminum foil
Load jars into perforated container and then into larger pot
fill pot up to half way up the side of the jars and cover, then bring to a boil and boil away or 90 minutes. Then unload jars and allow to cool
also i dont remove the foil until the minutes before im going to innoc, i also innoc through an alcohol soaked cotton ball.




from no-pc popcorn





--------------------
Admin @ mycotopia.net
Mycotopia


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InvisibleBrainiac
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Hippie3]
    #5626548 - 05/13/06 09:53 PM (17 years, 8 months ago)

Timetable of the tyndallization with some H202 process

Take out some of the water as it will be replace by the h2o2
1) Steam heating to 100 °C for 30 min
Vegetative cells are destroyed but endospores survive

2) Incubate at 30°C-37°C overnight
Most bacterial endospores germinate

3) Second heat treatment, 100 °C, 30 min
Germinated endospores are killed.

4) Second incubation at 30°C-37 °C overnight
Remaining endospores germinate

5) Third heat treatment, 100 °C, 60 min
Last remaining germinated endospores are killed.

When cool over night Inject  :syringe: the same amont of  h2o2 that you had taken out for H20

6 forth heat treatment ,100 c 60 min
Than the h2o2 sould be distrod
then you cont with inculation.
  :confused:Will this work ? :confused:


--------------------
:Awesketch:

:cool: Fair is Fair :devil:


Edited by Brainiac (05/13/06 09:55 PM)


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OfflineFGL
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Re: has somebody have success with pasteurizing grains or with fractional sterilization ? [Re: Brainiac]
    #5819726 - 07/04/06 12:06 PM (17 years, 6 months ago)

Quote:

Brainiac said:
Timetable of the tyndallization with some H202 process

Take out some of the water as it will be replace by the h2o2
1) Steam heating to 100 °C for 30 min
Vegetative cells are destroyed but endospores survive

2) Incubate at 30°C-37°C overnight
Most bacterial endospores germinate

3) Second heat treatment, 100 °C, 30 min
Germinated endospores are killed.

4) Second incubation at 30°C-37 °C overnight
Remaining endospores germinate

5) Third heat treatment, 100 °C, 60 min
Last remaining germinated endospores are killed.

When cool over night Inject  :syringe: the same amont of  h2o2 that you had taken out for H20

6 forth heat treatment ,100 c 60 min
Than the h2o2 sould be distrod
then you cont with inculation.
  :confused:Will this work ? :confused:




and what about adding the H2O2 in the water used to soak the grains? :rolleyes:


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