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OfflineFGL
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has somebody have success with pasteurizing grains or with fractional sterilization ?
    #5598096 - 05/06/06 04:11 PM (17 years, 8 months ago)

if so, how much time and what temp? :rolleyes:


Edited by FGL (05/12/06 04:23 PM)


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Offlineskeletor
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5598911 - 05/06/06 08:47 PM (17 years, 8 months ago)

pasturizing grains is useless. you need to sterilize them. this isn't just semantics either. you need those buggers devoid of life before you can use them as a substrate.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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InvisibleFooMan
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5598926 - 05/06/06 08:50 PM (17 years, 8 months ago)

If you're asking because you don't have a PC, then check out the link below. It's a PITA, but it does work.

http://www.shroomery.org/index.php/par/23372


--------------------

Quick WBS Prep


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InvisiblemushboyMDiscord
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Re: has somebody have success pasteurizing grains? [Re: FooMan]
    #5599269 - 05/06/06 10:40 PM (17 years, 8 months ago)

pita?


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OfflineShroomsnotWar
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Re: has somebody have success pasteurizing grains? [Re: mushboy]
    #5599271 - 05/06/06 10:41 PM (17 years, 8 months ago)

pain in the ass


--------------------
"Last time I checked, I was paying your salary" - me, to a cop, Feb 2000 ... yes I was arrested


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InvisiblemushboyMDiscord
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Re: has somebody have success pasteurizing grains? [Re: ShroomsnotWar]
    #5599275 - 05/06/06 10:42 PM (17 years, 8 months ago)

:dancing:
:dancing:
:dancing:
:dancing:
:dancing:
:dancing:
:dancing:
:dancing:
:dancing:


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: mushboy]
    #5600274 - 05/07/06 07:00 AM (17 years, 8 months ago)

Thanks everybody, but I have a pc BTW, I was just asking after reading this post:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5595140/an/0/page/0


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Invisibleohmatic
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5600377 - 05/07/06 08:32 AM (17 years, 8 months ago)

Quote:

FGL said:
Thanks everybody, but I have a pc BTW, I was just asking after reading this post:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5595140/an/0/page/0




mico is actually using a modified drum with a seal on lid that does create some pressure inside,
if you reasearched a bit more you would have even come across a picture of it :


--------------------
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RIP #cultivation! ....can't associate? well FUCK U !


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: ohmatic]
    #5600419 - 05/07/06 08:52 AM (17 years, 8 months ago)





mico is actually using a modified drum with a seal on lid that does create some pressure inside,
if you reasearched a bit more you would have even come across a picture of it :





Yes ohmatic, I have read the post where that picture appears http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5540921/an/0/page/1 and micolo2 says there "There is no gasket seal. So pressure stay low, very low. When pressure is starting to built, it goes out naturally between the cover and the drum. The valve is only for security. If you open the valve while boiling there is a pressure who blow steam out for about 18 inches for 2 or 3 seconds;You don't really have to use it. You could built one without valve. I've never seen pressure built up in the drum "


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InvisibleTippinthru
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5600841 - 05/07/06 12:00 PM (17 years, 8 months ago)

Pasteurizing grains, will not work.

But, you could try "Frac'ing".

From the FAQ.

"fractional sterilization" or tyndallization

Fractional sterilization or tyndallization is a method used to destroy bacteria and endospores in preparation of grain spawn(rye, wheat, millet, birdseed...).

In this case, the jars fitted with a filter disc, tyvek filter or a polyfill lid filter are boiled or steamed at 212°F (100°C) for 30 min in a pot with lid, three days in a row.

Between the boiling steps the jars are kept warm, around 30°C(but room temperature will work too), to allow the remaining endospores to germinate.

The basic principle behind this method is that any resistant endospores will germinate after the first heating and therefore be susceptible to killing during the second and third heating.

Timetable of the tyndallization (fractional sterilization) process

1) Steam heating to 100 °C for 30 min
Vegetative cells are destroyed but endospores survive

2) Incubate at 30°C-37°C overnight
Most bacterial endospores germinate

3) Second heat treatment, 100 °C, 30 min
Germinated endospores are killed.

4) Second incubation at 30°C-37 °C overnight
Remaining endospores germinate

5) Third heat treatment, 100 °C, 60 min
Last remaining germinated endospores are killed.


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: Tippinthru]
    #5600857 - 05/07/06 12:10 PM (17 years, 8 months ago)

thanks Tippinthru  :thumbup:

and the same question, has sombody have success with tyndallization? :grin:


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Offlineskeletor
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5601072 - 05/07/06 01:37 PM (17 years, 8 months ago)

yes but again its a huge pain in the ass. if you have a pc why would you bother?


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: skeletor]
    #5601159 - 05/07/06 02:08 PM (17 years, 8 months ago)

Quote:

skeletor said:
yes but again its a huge pain in the ass.  if you have a pc why would you bother?




because with a 55g drum you could do 100 jars (1liter), and while they are incubating you could do another 100 jars, and so on... :wink:


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5601170 - 05/07/06 02:11 PM (17 years, 8 months ago)

would be ok to do the fractional sterilization four times 2 hours any time to be sure???


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Offlinescatmanrav
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5601841 - 05/07/06 05:25 PM (17 years, 8 months ago)

You need 40 jars to do 100 wet pounds of poo..thats enough to do quite a bit of growing, and you could do that in 2 runs (or one) with some PC's..might cost you 200 bucks, but so what?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleFungusMan
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Re: has somebody have success pasteurizing grains? [Re: scatmanrav]
    #5601950 - 05/07/06 05:54 PM (17 years, 8 months ago)

I did many runs using fractural sterilization. I would soak and rinse the WBS as usual, then I would boil the jars 1 hour a day, for three days and innoc. I would also shake between boilings. Never had one contam. The only thing you have to watch for is moisture control. Add a little verm to help keep your jars from drying out. Never had any real problems doing it that way.


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: scatmanrav]
    #5602222 - 05/07/06 07:14 PM (17 years, 8 months ago)

Quote:

scatmanrav said:
You need 40 jars to do 100 wet pounds of poo..thats enough to do quite a bit of growing, and you could do that in 2 runs (or one) with some PC's..might cost you 200 bucks, but so what?




I know it scatmanrav, but I'd just like to know what others have learned about fractional sterilization because I think is a very interesting field to do some research, and havent found much info on the subject in the forums... :confused:


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OfflineFGL
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Re: has somebody have success pasteurizing grains? [Re: FungusMan]
    #5602243 - 05/07/06 07:20 PM (17 years, 8 months ago)

Quote:

FungusMan said:
I did many runs using fractural sterilization. I would soak and rinse the WBS as usual, then I would boil the jars 1 hour a day, for three days and innoc. I would also shake between boilings. Never had one contam. The only thing you have to watch for is moisture control. Add a little verm to help keep your jars from drying out. Never had any real problems doing it that way.




That is what I want to hear FungusMan  :thumbup: :thumbup: :thumbup:

do you think would be any problem if I do 4 runs instead of 3 and I want to do it in a 55g drum so I think it will take more time for getting the right temp in the whole substrate so maybe doing 3 to 4 hours runs would be better... or not? :rolleyes:

any opinion would be greatly appreciated  :grin: :mushroom2: :mushroom2: :mushroom2:


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Offlineskeletor
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5602678 - 05/07/06 09:06 PM (17 years, 8 months ago)

how do you plan to take 55g out and inject it and keep in sterile?


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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InvisibleFungusMan
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Re: has somebody have success pasteurizing grains? [Re: FGL]
    #5602736 - 05/07/06 09:17 PM (17 years, 8 months ago)

Quote:

FGL said:
Quote:

FungusMan said:
I did many runs using fractional sterilization. I would soak and rinse the WBS as usual, then I would boil the jars 1 hour a day, for three days and innoc. I would also shake between boilings. Never had one contam. The only thing you have to watch for is moisture control. Add a little verm to help keep your jars from drying out. Never had any real problems doing it that way.




That is what I want to hear FungusMan  :thumbup: :thumbup: :thumbup:

do you think would be any problem if I do 4 runs instead of 3 and I want to do it in a 55g drum so I think it will take more time for getting the right temp in the whole substrate so maybe doing 3 to 4 hours runs would be better... or not? :rolleyes:

any opinion would be greatly appreciated  :grin: :mushroom2: :mushroom2: :mushroom2:




As long as you can keep the water boiling, 1 hour runs should be fine. The longer the runs, the more chance of grains splitting and turning to mush.Also let me add that, when boiling my jars, the jars were only HALF submersed. So, I really don't know how to apply this to drums, unless you stacked the jars in the barrel, and steamed them, in which I would do 4 runs of 1.5 hours, shaking each before each steam session.


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