|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
frankdog7
Moldy Thumb


Registered: 05/02/06
Posts: 44
Loc: USA
Last seen: 17 years, 7 months
|
semi-newbie, i dont' know what went wrong..
#5597569 - 05/06/06 11:43 AM (17 years, 8 months ago) |
|
|
Ok, here it is. I baked the vermiculite- 8 cups (not the course kind) and the brown rice flower ( 3cups) at 300 deg. for 20 min. i also baked ex i boiled more water than needed and mesured out 3 cups of hot water and mixed it all together (after i mixed the verm and b.r. together). even before all that i lysol'd the work area and turned off the a/c. prepped myself with breathing mask, 91% alcohol cleaned latex gloves. I even took the nail that i used to puncture the lids with and lit it with a lighter so i could make sure there would be no contaminants on it while i was hammering. I pressure cooked 4 jars at a time in a 5 qt 11psi p.c. for 45 min- 1 hr. I sterilized the top of the lids with alcohol and covered it with foil to make sure nothing would get in them. I waited 24 hrs for cooling time to inject my refrigerated syringes. I once again prepped myself and the needle with a quick swab of alcohol and injected accordingly. After injection i put them in an incubator filled 3/4 to the top of the jar at 80 deg. all this for no growth. i realize sterility is a HUGE part in this project..I know i did way more than the norm as far as prepping goes. what did i do? i haven't seen growth in 2 batches..
-------------------- "I will never run out in the street naked again"
|
Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 16 hours
|
Re: semi-newbie, i dont' know what went wrong.. [Re: frankdog7]
#5597603 - 05/06/06 12:00 PM (17 years, 8 months ago) |
|
|
You are overdoing it a bit. No need for baking vermiculite, brown rice, hot water......
Just sterilize it for 1 hour at 10 psi and it should be ok.
By all you wrote though, your syringes might be bunk too.
|
frankdog7
Moldy Thumb


Registered: 05/02/06
Posts: 44
Loc: USA
Last seen: 17 years, 7 months
|
Re: semi-newbie, i dont' know what went wrong.. [Re: Anno]
#5597614 - 05/06/06 12:06 PM (17 years, 8 months ago) |
|
|
i thought about that but i thought i got them froma relaible source....
-------------------- "I will never run out in the street naked again"
|
Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 16 hours
|
Re: semi-newbie, i dont' know what went wrong.. [Re: frankdog7]
#5597652 - 05/06/06 12:28 PM (17 years, 8 months ago) |
|
|
Try the PF-Tek for Simple Minds in my signature, you might also do some grain jars since you have a pressure cooker.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 364 topic views. 17 members, 155 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|