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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinefrankdog7
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Registered: 05/02/06
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semi-newbie, i dont' know what went wrong..
    #5597569 - 05/06/06 11:43 AM (17 years, 8 months ago)

Ok, here it is. I baked the vermiculite- 8 cups (not the course kind) and the brown rice flower ( 3cups) at 300 deg. for 20 min. i also baked ex i boiled more water
than needed and mesured out 3 cups of hot water and mixed it all together (after i mixed the verm and b.r. together). even before all that
i lysol'd the work area and turned off the a/c. prepped myself with breathing mask, 91% alcohol cleaned latex gloves. I even took the nail that i
used to puncture the lids with and lit it with a lighter so i could make sure there would be no contaminants on it while i was hammering.
I pressure cooked 4 jars at a time in a 5 qt 11psi p.c. for 45 min- 1 hr. I sterilized the top of the lids with alcohol and covered it with foil to make sure
nothing would get in them. I waited 24 hrs for cooling time to inject my refrigerated syringes. I once again prepped myself and the needle with a quick
swab of alcohol and injected accordingly. After injection i put them in an incubator filled 3/4 to the top of the jar at 80 deg. all this for no growth. i
realize sterility is a HUGE part in this project..I know i did way more than the norm as far as prepping goes. what did i do? i haven't seen growth in
2 batches..


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"I will never run out in the street naked again"


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OfflineAnnoA
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Re: semi-newbie, i dont' know what went wrong.. [Re: frankdog7]
    #5597603 - 05/06/06 12:00 PM (17 years, 8 months ago)

You are overdoing it a bit.
No need for baking vermiculite, brown rice, hot water......

Just sterilize it for 1 hour at 10 psi and it should be ok.

By all you wrote though, your syringes might be bunk too.


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Offlinefrankdog7
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Registered: 05/02/06
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Last seen: 17 years, 7 months
Re: semi-newbie, i dont' know what went wrong.. [Re: Anno]
    #5597614 - 05/06/06 12:06 PM (17 years, 8 months ago)

i thought about that but i thought i got them froma relaible source....


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"I will never run out in the street naked again"


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OfflineAnnoA
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Registered: 06/17/99
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Re: semi-newbie, i dont' know what went wrong.. [Re: frankdog7]
    #5597652 - 05/06/06 12:28 PM (17 years, 8 months ago)

Try the PF-Tek for Simple Minds in my signature, you might also do some grain jars since you have a pressure cooker.


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