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InvisibletrendalM
J♠
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Registered: 04/17/01
Posts: 20,815
Loc: Ontario, Canada
first try: 12 jars made....12 jars lost.
    #559664 - 02/22/02 12:23 PM (22 years, 4 days ago)

Made 12 jars using the PF tek and innoculated with B+ spores. The first signs of germination occured after 5 days, but most of the jars took 10 days to germinate. Lost 2 jars to green mold in the first 5 days.

And then today. I had noticed about a week ago that there was a "red tint" in a lot of the jars. The tint was fairly light, not bright, and did not appear to grow in size or change at all. Today I threw out the remaining 10 jars. The red tint had gotten much darker, in some spots turning almost a bright orange. A few jars had some lipstick mold growing in them. Every jar smelled like old gym socks. A couple jars had some nasty looking yellow globs in them. The globs looked like little bumpy and slimy yellow balls.

Funny thing is, there were no signs of more green mold. Isn't green mold supposed to be the most common contaminant? Looks like I got everything except green mold.


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Once, men turned their thinking over to machines in the hope that this would set them free.
But that only permitted other men with machines to enslave them.


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 14 days, 14 hours
Re: first try: 12 jars made....12 jars lost. [Re: trendal]
    #559677 - 02/22/02 12:34 PM (22 years, 4 days ago)

It just depends... green mold is very common for lots of people, but is one of the few that my friend has never caught. Be real careful with those yellows, they could be pretty nasty (make you sick).

What kind of sterilization protocols did you use? It could be bad luck if you lose a jar or two to a single type of contamination, but with all the different ones you have in all of the jars, there is probably a problem in your technique somewhere.

Good luck with round two!



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Just another spore in the wind.


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InvisibletrendalM
J♠
Male User Gallery

Registered: 04/17/01
Posts: 20,815
Loc: Ontario, Canada
Re: first try: 12 jars made....12 jars lost. [Re: Seuss]
    #559683 - 02/22/02 12:39 PM (22 years, 4 days ago)

Yeah, next time I'm not going to get high before making the jars.

I tried to be as sterile as I could. Covered the kitchen door with plastic. Blocked off the air vents. Sprayed down the walls, stove, and counter with bleech and water.

Two things I wish I had of done: use a pc instead of just boiling the jars for an hour, and flame my needle in between innoculations instead of just wipe it with alcohol.


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Once, men turned their thinking over to machines in the hope that this would set them free.
But that only permitted other men with machines to enslave them.


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OfflineMilhouseT1
somewhatexperienced
Registered: 02/17/02
Posts: 46
Loc: Philly, PA
Last seen: 11 years, 8 months
Re: first try: 12 jars made....12 jars lost. [Re: trendal]
    #560536 - 02/23/02 10:06 AM (22 years, 3 days ago)

You should go buy a pressure cooker before you try again


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Offlinemelic
undead
Registered: 02/21/02 Happy 22nd Shroomiversary!
Posts: 85
Loc: fin
Last seen: 18 years, 10 months
Re: first try: 12 jars made....12 jars lost. [Re: trendal]
    #562753 - 02/25/02 03:25 PM (22 years, 1 day ago)

I wasn't even nearly as carefull as you with the bunch o' jars I have growing right now.. and one of em has some large yellow stuff in it, looks kinda like myc. though... still have a feeling ill loose em all.


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    Look at that bunch over there man, They've spotted us !

Melic  :tongue:


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