Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Grow Bags

Jump to first unread post Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Sterilizing grain in a 55 gal. drum
    #5595140 - 05/05/06 06:08 PM (17 years, 8 months ago)

I've made this interesting experience. Trying to sterilize wheat grain in a 55 gal. barril. If it works, it'll be possible to sterilize more than 120 of 1 litre jars or, more than 250-500ml. jars. For this experience I've used 22-1 litre jars. In the bottom of the drum was sawdust bags, 2nd shelf the grain jars and the first shelf other sawdust bags to fill the drum. My expactations are negative but could change?

Here's a picture of the jars in the drum. They have being inoculated the 1st of may.



Now, may 5th. I've inoculated for the experience Eryngii, Blue Oyster, Cauliflower mush. and White oyster. You can see the growth of the mycelium (Eryngii for the photo), no sign of contamination yet.



I esitate to brake the kernels, as you know why. Would you brake the kernels or wait to the entire colonization?

Does someone is interested to know more how it'll go on this experience?


Extras: Filter Print Post Top
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 7 years, 27 days
Trusted Cultivator
Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
    #5595198 - 05/05/06 06:26 PM (17 years, 8 months ago)

hey micololo2 - happy st. george's day  :laugh:

first - 120 litre jars sterilised in one go in just a barrel deserves credit and must unfortunately also come with a warning about known areas of diminished sterility dependent on oven shape, degree of filling and design issues. but you know this. crap, what i really mean is well done sir  :thumbup:

second - as for shaking the kernels, you will read all sorts of advice, but the average  seems to be that at least one good shake is recommended at 5-10% (about now), and after that leave well alone until full colonisation.

third - how do you guarantee you will wake up with a smile on your face? go to sleep with a coathanger in your mouth  :cool: :smile:


--------------------
buh


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Sterilizing grain in a 55 gal. drum [Re: shirley knott]
    #5595354 - 05/05/06 07:09 PM (17 years, 8 months ago)

Hi Sherly
Thanks for the advice, I'll try it tonight. If you're telling me this it's probably because you have experimented it!
What is St. George day. Who's George?
I prefer the look you had before than the one you got now.


Extras: Filter Print Post Top
OfflineJeremy_Davis
Mycelial NetworkAdministrator
Male User Gallery

Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 11 years, 9 months
Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
    #5596312 - 05/05/06 11:12 PM (17 years, 8 months ago)

Man, this is amazing, I think it will mean a great deal to those in third world countries (that our ECHO farm primarily helps), and may mean obviously more food and more jobs where they are needed most, as well as in impoverished areas in developed nations. I am intensely interested in this.
Can you elaborate on how it was done? Time spent in the drum, everything you have time to tell us. I LOVE your posts! Great work man, you are an innovator and I can't wait to see how it comes out.
Light and Love,
JD


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta


Extras: Filter Print Post Top
OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 4 days
Re: Sterilizing grain in a 55 gal. drum [Re: Jeremy_Davis]
    #5596896 - 05/06/06 03:14 AM (17 years, 8 months ago)

:thumbup:

Amazing.

Hope it goes well :cool:

-Gnostic


Extras: Filter Print Post Top
OfflineFreeSporePrints
Male User Gallery
Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Sterilizing grain in a 55 gal. drum [Re: IGnosticAbhorI]
    #5597325 - 05/06/06 09:51 AM (17 years, 8 months ago)

Hi micololo,
you've done a wonderfull job.

i do not use to shake them...for me this cause stress..in the natural environment nobody shakes the mycelium, perhaps just some worms..but it's just my opinion.

If you can add new photos and let us updated! ^_^


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Sterilizing grain in a 55 gal. drum [Re: Jeremy_Davis]
    #5597834 - 05/06/06 02:18 PM (17 years, 8 months ago)

Quote:

Jeremy_Davis said:
Man, this is amazing, I think it will mean a great deal to those in third world countries (that our ECHO farm primarily helps), and may mean obviously more food and more jobs where they are needed most, as well as in impoverished areas in developed nations. I am intensely interested in this.
Can you elaborate on how it was done? 4.5 gallons of water in the drumTime spent in the drum, 170 minutes boilingeverything you have time to tell us. I LOVE your posts! Thanks, I'll come back with other ideas in other postsGreat work man, you are an innovator and I can't wait to see how it comes out.
Light and Love,
JD




I've shaking the kernels today, hopping it wont wake up contamination. I just kept one jars without being shaked. I just regrett that I have not kept more jars without being shaked, to compare the results in the experience. I,ll come back in a few days with some results. Freesporeprint I just read your post to late.

I'd love to go in far countries to meet different difficulties and trying to find simple and cheap ways to grow mushrooms in medium scale, in different environments.


Extras: Filter Print Post Top
OfflineFGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
    #5598029 - 05/06/06 03:40 PM (17 years, 8 months ago)

did you use the 55 gal. drum as a pc? :runaway:  or just tried to pasteurized the grain for a long time?


Extras: Filter Print Post Top
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 7 years, 27 days
Trusted Cultivator
Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
    #5600106 - 05/07/06 03:41 AM (17 years, 8 months ago)

he's using it as a steam steriliser - pasteurising cannot go above about 75C.

btw, St George is the patron saint of England. not much is known for definite about him (he was killed in about year 300) but his name is used in various old texts, Shakespeare referred to him lustily as someone to name when riding into battle, and he is usually portrayed for some reason on horeseback killing a dragon. nobody really celebrates St George's day in England.



it is St George's flag (red cross on white background) that is the English flag, and which when combined with the Scottish flag (St Andrew's, white cross on blue background) and Irish flags (St Patrick's, red diagonal cross on white background) makes up the well-known 'Union Flag' (also known as the 'Union Jack', though more accurately this only applies when it is flown from a British warship).

+ + =


--------------------
buh


Extras: Filter Print Post Top
OfflineFGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
Re: Sterilizing grain in a 55 gal. drum [Re: shirley knott]
    #5600277 - 05/07/06 07:02 AM (17 years, 8 months ago)

Quote:

shirley knott said:
he's using it as a steam steriliser - pasteurising cannot go above about 75C.




So steam sterilization without pressure???

Please micolo2 more details!  :eek:


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 52 minutes
Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
    #5600543 - 05/07/06 09:56 AM (17 years, 8 months ago)



Extras: Filter Print Post Top
OfflineFGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
    #5600599 - 05/07/06 10:17 AM (17 years, 8 months ago)

I read itr before, but I think micolo is pasteurizing there, no seterilizing  :banghead:


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
    #5601538 - 05/07/06 04:08 PM (17 years, 8 months ago)

You can call it as you want. I don't know if I,m sterilizing or pasteurizing. What I know, there is a low pressure in the drum because when I open the valve steam blows for 1-2 seconds. I don't know what temperature is reached in the drum and I still don't know if it's going to work.
Later+


Extras: Filter Print Post Top
OfflineFreeSporePrints
Male User Gallery
Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
    #5603579 - 05/08/06 12:54 AM (17 years, 8 months ago)

hi FGL, if we want to be precise the great job that our friend micololo2 did is neither a sterilization nor a pasteurization: i believe it's a mix of both the process.

The sterilization is let by the heat and the pressure (the drum of micololo doesn't reach high pressures) and the pasteurization is let by the several boiling of the substrate, with pauses between a boil and a boil, to permit to the bacterias to born and next killing them.

A part this boring explanation, we all hope that the "killer-can" (please micololo call it Killer-can ehehe) works ok to permit an adeguate "sterilization" (better to say "bacteriological holocaust" ) in order that mycelium can grow without enemies...and sincery i believed that micololo hitted the centre..but we have to wait a bit of days, until the jars or the bags are fully colonized.

Have a nice day!! ^_^


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 52 minutes
Re: Sterilizing grain in a 55 gal. drum [Re: FreeSporePrints]
    #5604044 - 05/08/06 05:15 AM (17 years, 8 months ago)

>and the pasteurization is let by the several boiling of the substrate, with
>pauses between a boil and a boil, to permit to the bacterias to born and next
>killing them.

No. What you are describing is "fractional sterilization" or "tyndalization".

Pasteurization is heating a substrate to a temperature of ~70°C (160°F).


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
    #5604314 - 05/08/06 09:23 AM (17 years, 8 months ago)

7 days after inoculation, looks like it's going to work.



hundreds of sawdust-woodchips bags being sterilize with the drum



Extras: Filter Print Post Top
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
    #5604752 - 05/08/06 12:05 PM (17 years, 8 months ago)

wow wish you best luck that it works out !
that would be a "super-low-cost-quasi-autoklav" there :wink:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Extras: Filter Print Post Top
Offlinebluelou
NUTCASEdrugbucket!
Male User Gallery

Registered: 05/11/02
Posts: 1,086
Loc: $hroom Central
Last seen: 6 years, 4 months
Re: Sterilizing grain in a 55 gal. drum [Re: ohmatic]
    #5605214 - 05/08/06 02:37 PM (17 years, 8 months ago)

FUCKIN NUTZZZZZZ!!!!!!!!

And i thought it was about commercial buttons or ports?
COOL looks like you got away with it,that is MASSIVE!!!!!!
peace!


--------------------
Have you tried my(black kow) pile style tek outdoors!!!!!!!!


Extras: Filter Print Post Top
OfflineFreeSporePrints
Male User Gallery
Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Sterilizing grain in a 55 gal. drum [Re: bluelou]
    #5607420 - 05/09/06 02:38 AM (17 years, 8 months ago)

hi Anno, you're right! i wrong. :smile:

micololo great great great job! i believe i'll built my killer-can in the future!

yeah!!


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
    #5616668 - 05/11/06 10:43 AM (17 years, 8 months ago)

Hey Friends I got great, great great news.
The experience is positive. 22 jars of grain, after 10 days being "tyndalized" 170min.,inoculated with 4 different strains, this is the results:
8-Pl. Eryngii: 6 full colonized, 2 slow growing(due to lower inoculation rate)
7-Pl. Ostreatus (white): 6 full colonized, 1 no growth
4- Pl. Colombinus: 4 full colonized
3- Sparasis Crispa: 2- 30% of growth and 1 infected.

All full colonized are ready to use.

Anyone can grow hundreds of Pds, of mush. per week naturaly whithout investing thousands and thousands of dollars. Just adapt the right species at the right time of the year with some personnal strategies in the the environment where they grow. Now,I do not have any filter, machines, flow hoods ... Only fans and a glove box. Almost all innoculations work are made outdoors with a high rate of success.
Treat your mycelium strain as an olympian, you're the trainer. Always give him the best food, the best timings to get the best athlete and you'll win the game.

Good luck all of you

BTW here some flushes recently picked.



Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* Bulk Pasteurization of Straw in 55 Gallon Drum mattymonkey 7,407 11 05/11/05 05:59 AM
by msmushrooms
* SHIITAKE GRAIN MASTER BAG PURCHASED FROM FUNGIPERFECTI.COM MickeyMsi 6,148 15 12/02/03 10:11 AM
by psilocyber
* small scale commercial production questions
( 1 2 3 4 all )
fungophiliac 16,450 74 03/21/05 11:08 AM
by fungophiliac
* Pasteurization Vessels and the Law YidakiMan 2,660 10 05/23/04 07:46 PM
by matts
* SHROOMSISAY01 55 GALLON STEAMER TEK.
( 1 2 3 4 all )
SHROOMSISAY01M 12,228 67 05/04/21 02:13 PM
by bodhisatta
* FungiPerf Oyster Patch: Straw To Grain to make spawn?
( 1 2 all )
Aneglakya 8,424 34 06/14/04 03:39 AM
by shirley knott
* Creating grain spawn with the peroxide method ragadinks 9,188 16 03/22/05 02:30 AM
by jmeuser
* For slow growing gourmets, pasteurize or sterilize substrate you will spawn to? Chastity_Belt 2,495 6 06/07/05 12:04 PM
by Chastity_Belt

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
10,661 topic views. 2 members, 10 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.004 seconds on 14 queries.