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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Sterilizing grain in a 55 gal. drum
#5595140 - 05/05/06 06:08 PM (17 years, 8 months ago) |
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I've made this interesting experience. Trying to sterilize wheat grain in a 55 gal. barril. If it works, it'll be possible to sterilize more than 120 of 1 litre jars or, more than 250-500ml. jars. For this experience I've used 22-1 litre jars. In the bottom of the drum was sawdust bags, 2nd shelf the grain jars and the first shelf other sawdust bags to fill the drum. My expactations are negative but could change?
Here's a picture of the jars in the drum. They have being inoculated the 1st of may.

Now, may 5th. I've inoculated for the experience Eryngii, Blue Oyster, Cauliflower mush. and White oyster. You can see the growth of the mycelium (Eryngii for the photo), no sign of contamination yet.

I esitate to brake the kernels, as you know why. Would you brake the kernels or wait to the entire colonization?
Does someone is interested to know more how it'll go on this experience?
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shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 27 days
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Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
#5595198 - 05/05/06 06:26 PM (17 years, 8 months ago) |
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hey micololo2 - happy st. george's day 
first - 120 litre jars sterilised in one go in just a barrel deserves credit and must unfortunately also come with a warning about known areas of diminished sterility dependent on oven shape, degree of filling and design issues. but you know this. crap, what i really mean is well done sir 
second - as for shaking the kernels, you will read all sorts of advice, but the average seems to be that at least one good shake is recommended at 5-10% (about now), and after that leave well alone until full colonisation.
third - how do you guarantee you will wake up with a smile on your face? go to sleep with a coathanger in your mouth
-------------------- buh
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Sterilizing grain in a 55 gal. drum [Re: shirley knott]
#5595354 - 05/05/06 07:09 PM (17 years, 8 months ago) |
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Hi Sherly Thanks for the advice, I'll try it tonight. If you're telling me this it's probably because you have experimented it! What is St. George day. Who's George? I prefer the look you had before than the one you got now.
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Jeremy_Davis
Mycelial NetworkAdministrator


Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 11 years, 9 months
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Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
#5596312 - 05/05/06 11:12 PM (17 years, 8 months ago) |
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Man, this is amazing, I think it will mean a great deal to those in third world countries (that our ECHO farm primarily helps), and may mean obviously more food and more jobs where they are needed most, as well as in impoverished areas in developed nations. I am intensely interested in this. Can you elaborate on how it was done? Time spent in the drum, everything you have time to tell us. I LOVE your posts! Great work man, you are an innovator and I can't wait to see how it comes out. Light and Love, JD
-------------------- Jeremy Davis Educational Concerns for Hunger Organization, Inc. Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta
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IGnosticAbhorI
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Registered: 11/06/04
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Re: Sterilizing grain in a 55 gal. drum [Re: Jeremy_Davis]
#5596896 - 05/06/06 03:14 AM (17 years, 8 months ago) |
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Amazing.
Hope it goes well 
-Gnostic
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FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 1 month, 26 days
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Re: Sterilizing grain in a 55 gal. drum [Re: IGnosticAbhorI]
#5597325 - 05/06/06 09:51 AM (17 years, 8 months ago) |
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Hi micololo, you've done a wonderfull job.
i do not use to shake them...for me this cause stress..in the natural environment nobody shakes the mycelium, perhaps just some worms..but it's just my opinion.
If you can add new photos and let us updated! ^_^
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Sterilizing grain in a 55 gal. drum [Re: Jeremy_Davis]
#5597834 - 05/06/06 02:18 PM (17 years, 8 months ago) |
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Quote:
Jeremy_Davis said: Man, this is amazing, I think it will mean a great deal to those in third world countries (that our ECHO farm primarily helps), and may mean obviously more food and more jobs where they are needed most, as well as in impoverished areas in developed nations. I am intensely interested in this. Can you elaborate on how it was done? 4.5 gallons of water in the drumTime spent in the drum, 170 minutes boilingeverything you have time to tell us. I LOVE your posts! Thanks, I'll come back with other ideas in other postsGreat work man, you are an innovator and I can't wait to see how it comes out. Light and Love, JD
I've shaking the kernels today, hopping it wont wake up contamination. I just kept one jars without being shaked. I just regrett that I have not kept more jars without being shaked, to compare the results in the experience. I,ll come back in a few days with some results. Freesporeprint I just read your post to late.
I'd love to go in far countries to meet different difficulties and trying to find simple and cheap ways to grow mushrooms in medium scale, in different environments.
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
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Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
#5598029 - 05/06/06 03:40 PM (17 years, 8 months ago) |
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did you use the 55 gal. drum as a pc? or just tried to pasteurized the grain for a long time?
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shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 27 days
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Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
#5600106 - 05/07/06 03:41 AM (17 years, 8 months ago) |
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he's using it as a steam steriliser - pasteurising cannot go above about 75C.
btw, St George is the patron saint of England. not much is known for definite about him (he was killed in about year 300) but his name is used in various old texts, Shakespeare referred to him lustily as someone to name when riding into battle, and he is usually portrayed for some reason on horeseback killing a dragon. nobody really celebrates St George's day in England.

it is St George's flag (red cross on white background) that is the English flag, and which when combined with the Scottish flag (St Andrew's, white cross on blue background) and Irish flags (St Patrick's, red diagonal cross on white background) makes up the well-known 'Union Flag' (also known as the 'Union Jack', though more accurately this only applies when it is flown from a British warship).
+ + =
-------------------- buh
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FGL
Stranger

Registered: 06/25/05
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Re: Sterilizing grain in a 55 gal. drum [Re: shirley knott]
#5600277 - 05/07/06 07:02 AM (17 years, 8 months ago) |
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Quote:
shirley knott said: he's using it as a steam steriliser - pasteurising cannot go above about 75C.
So steam sterilization without pressure???
Please micolo2 more details!
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 52 minutes
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Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
#5600543 - 05/07/06 09:56 AM (17 years, 8 months ago) |
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FGL
Stranger

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Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
#5600599 - 05/07/06 10:17 AM (17 years, 8 months ago) |
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I read itr before, but I think micolo is pasteurizing there, no seterilizing
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Sterilizing grain in a 55 gal. drum [Re: FGL]
#5601538 - 05/07/06 04:08 PM (17 years, 8 months ago) |
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You can call it as you want. I don't know if I,m sterilizing or pasteurizing. What I know, there is a low pressure in the drum because when I open the valve steam blows for 1-2 seconds. I don't know what temperature is reached in the drum and I still don't know if it's going to work. Later+
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FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 1 month, 26 days
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Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
#5603579 - 05/08/06 12:54 AM (17 years, 8 months ago) |
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hi FGL, if we want to be precise the great job that our friend micololo2 did is neither a sterilization nor a pasteurization: i believe it's a mix of both the process.
The sterilization is let by the heat and the pressure (the drum of micololo doesn't reach high pressures) and the pasteurization is let by the several boiling of the substrate, with pauses between a boil and a boil, to permit to the bacterias to born and next killing them.
A part this boring explanation, we all hope that the "killer-can" (please micololo call it Killer-can ehehe) works ok to permit an adeguate "sterilization" (better to say "bacteriological holocaust" ) in order that mycelium can grow without enemies...and sincery i believed that micololo hitted the centre..but we have to wait a bit of days, until the jars or the bags are fully colonized.
Have a nice day!! ^_^
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 52 minutes
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Re: Sterilizing grain in a 55 gal. drum [Re: FreeSporePrints]
#5604044 - 05/08/06 05:15 AM (17 years, 8 months ago) |
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>and the pasteurization is let by the several boiling of the substrate, with >pauses between a boil and a boil, to permit to the bacterias to born and next >killing them.
No. What you are describing is "fractional sterilization" or "tyndalization".
Pasteurization is heating a substrate to a temperature of ~70°C (160°F).
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
#5604314 - 05/08/06 09:23 AM (17 years, 8 months ago) |
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7 days after inoculation, looks like it's going to work.

hundreds of sawdust-woodchips bags being sterilize with the drum
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Sterilizing grain in a 55 gal. drum [Re: micololo2]
#5604752 - 05/08/06 12:05 PM (17 years, 8 months ago) |
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wow wish you best luck that it works out ! that would be a "super-low-cost-quasi-autoklav" there
--------------------
MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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bluelou
NUTCASEdrugbucket!


Registered: 05/11/02
Posts: 1,086
Loc: $hroom Central
Last seen: 6 years, 4 months
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Re: Sterilizing grain in a 55 gal. drum [Re: ohmatic]
#5605214 - 05/08/06 02:37 PM (17 years, 8 months ago) |
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FUCKIN NUTZZZZZZ!!!!!!!!
And i thought it was about commercial buttons or ports? COOL looks like you got away with it,that is MASSIVE!!!!!! peace!
-------------------- Have you tried my(black kow) pile style tek outdoors!!!!!!!!
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FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 1 month, 26 days
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Re: Sterilizing grain in a 55 gal. drum [Re: bluelou]
#5607420 - 05/09/06 02:38 AM (17 years, 8 months ago) |
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hi Anno, you're right! i wrong. 
micololo great great great job! i believe i'll built my killer-can in the future!
yeah!!
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Sterilizing grain in a 55 gal. drum [Re: Anno]
#5616668 - 05/11/06 10:43 AM (17 years, 8 months ago) |
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Hey Friends I got great, great great news. The experience is positive. 22 jars of grain, after 10 days being "tyndalized" 170min.,inoculated with 4 different strains, this is the results: 8-Pl. Eryngii: 6 full colonized, 2 slow growing(due to lower inoculation rate) 7-Pl. Ostreatus (white): 6 full colonized, 1 no growth 4- Pl. Colombinus: 4 full colonized 3- Sparasis Crispa: 2- 30% of growth and 1 infected.
All full colonized are ready to use.
Anyone can grow hundreds of Pds, of mush. per week naturaly whithout investing thousands and thousands of dollars. Just adapt the right species at the right time of the year with some personnal strategies in the the environment where they grow. Now,I do not have any filter, machines, flow hoods ... Only fans and a glove box. Almost all innoculations work are made outdoors with a high rate of success. Treat your mycelium strain as an olympian, you're the trainer. Always give him the best food, the best timings to get the best athlete and you'll win the game.
Good luck all of you
BTW here some flushes recently picked.
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