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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
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what is the best way to fight airborne comtamination?
#5594227 - 05/05/06 01:37 PM (17 years, 8 months ago) |
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Is to spray a bleach solution in the lab and to work in front of a hepa unit enough?
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: what is the best way to fight airborne comtamination? [Re: FGL]
#5595552 - 05/05/06 08:07 PM (17 years, 8 months ago) |
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Not a hepa unit if you mean a household stand alone filter. You need a laminar flow hood. I used to mist a ten percent bleach solution in the air, but got tired of burning my eyes everytime I had to do sterile work. Now I spray the room with Oust until it's a fog. Let the fog settle, then repeat. After the second Oust treatment settles, turn on the laminar flow hood and allow it to run for an hour before beginning sterile work.
It's also important to remember we humans are the number one vector of contamination during sterile work, not the air. Wear gloves, mask, etc. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
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Re: what is the best way to fight airborne comtamination? [Re: RogerRabbit]
#5597109 - 05/06/06 07:31 AM (17 years, 8 months ago) |
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Thanks for your help Roger 
some questions:
Does Oust kill mold spores in the air or just bacteria?
Is safe to spray a 10% bleach solution to clean surfaces after spraying Oust?
Thanks
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: what is the best way to fight airborne comtamination? [Re: FGL]
#5597517 - 05/06/06 11:21 AM (17 years, 8 months ago) |
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Both mold and bacteria. You can still use bleach, but I prefer to wipe down the surfaces I work on, as well as my latex gloves with 70% alcohol. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
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Re: what is the best way to fight airborne comtamination? [Re: RogerRabbit]
#5597599 - 05/06/06 11:59 AM (17 years, 8 months ago) |
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Thanks!!!
So the mix of bleach and Oust dont create dangerous gases, isnt it?
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: what is the best way to fight airborne comtamination? [Re: FGL]
#5599050 - 05/06/06 09:35 PM (17 years, 8 months ago) |
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FLow hood would be ideal as rabbit suggests - a cheaper otion and one that worked for me was a glovebox sprayed with bleach 20 minutes before use - i dont like using chemicals but when i had a bad contam outbreak it was my only choice considering what I had to spend.
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
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Re: what is the best way to fight airborne comtamination? [Re: Zen Peddler]
#5604783 - 05/08/06 12:12 PM (17 years, 8 months ago) |
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Quote:
bluemeanie said: FLow hood would be ideal as rabbit suggests - a cheaper otion and one that worked for me was a glovebox sprayed with bleach 20 minutes before use - i dont like using chemicals but when i had a bad contam outbreak it was my only choice considering what I had to spend.
Thanks
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alsnow469
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Registered: 10/15/05
Posts: 392
Last seen: 6 months, 13 days
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Re: what is the best way to fight airborne comtamination? [Re: FGL]
#5611425 - 05/10/06 02:08 AM (17 years, 8 months ago) |
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Oustenatedcloride
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