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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinebadlarryumd
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Registered: 09/25/05
Posts: 22
Last seen: 17 years, 1 day
is my syringe dirty?
    #5593971 - 05/05/06 12:34 PM (17 years, 8 months ago)

can't get jars to successfully colinate via LC inoculation. major stalling noticed time after time. got a specific question hoping someone could answer.

the other day some WBS jars were knocked up with an LC. Afterwards it was noticed that the syringe used had some brown dirty looking shit stuck to the plastic axis that operates the plunger.

could this be a source of contamination?

keep in mind the syringe was PCed for 90minutes with the grain right before it was used.

I suppose this a dumb question b/c in the future the syringe will be cleaned before PCing to eliminate any doubt, but i was curious of opinions i guess. heavily troubled with failure lately.

thanks
happy cinco de mayo :eek:


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Offlineiamyour_messiah
Stranger

Registered: 03/07/06
Posts: 273
Last seen: 4 years, 1 month
Re: is my syringe dirty? [Re: badlarryumd]
    #5594045 - 05/05/06 12:50 PM (17 years, 8 months ago)

I don't have an answer for you, sadly. But what I can say, is that although technically any bacteria or whatever in there ought to be dead after 90 mins in a PC like you said....for me my mind would try to convince itself that its dirty and a source of contamination. Cause say it was some bacteria, and it got killed in the PC, would the remains of the bacteria be enough to cause problems in the future? Pretty interesting question actually..

I had a similar experience recently when made an LC in this jar that originally had asparagus in it and I kept cleaning it hardcore with saop and warm water to get rid of any trace of an asparagus smell. But in the end its like.....if its been cleaned once, and it going into a pressure cooker, will that actually make a difference if theres a bit of a lingering smell. But its all psychological I think... although I still attribute the failure of that LC to the fact it was an asparagus jar :P


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