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IwantELVES
ChronoSynclasticInfundibulumated


Registered: 02/08/06
Posts: 63
Last seen: 16 years, 2 months
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Less light = More pins?
#5593195 - 05/05/06 08:29 AM (17 years, 8 months ago) |
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Sorry for my redudnancy, but I didn't get the response I wished for in my last thread.
Anyway, I have noticed that I am getting more pins when I remove my culture from light after pinning. That is the only time I use light is to initiate pinning.
I have tried various light sources and the time exposed to light. Each time, the one in darkness yields more.
Some memebers who posted in my previous thread doubt that the lighting is the biggest factor, but what else could it be?
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MLBjammer
Invitro Freak


Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
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Re: Less light = More pins? [Re: IwantELVES]
#5593310 - 05/05/06 09:17 AM (17 years, 8 months ago) |
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Well moisture and humidity and air exchange are the big three. The light factor--you prolly know--triggers the pins to grow. Most experts would suggest 12 hrs of light/12 hrs of dark--replicating a semi-natural environment. Are you putting your grows in something else after pinning starts . . .
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Less light = More pins? [Re: IwantELVES] 1
#5593317 - 05/05/06 09:19 AM (17 years, 8 months ago) |
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Many things. I've found the brightest light stimulates more pins. You need to look at pinning triggers like the instruments in a band. One instrument can be slightly off, and the band still plays the song. Often one instrument can be taken away and the music still sounds ok. However, if all are working together, it's awesome.
Perhaps the projects you remove from light after exposure are mainaining a higher humidity due to no heat from the lights. Humidity is a pinning trigger just like light. Maybe the ones you remove from the lights get better air circulation. Fresh air is a pinning trigger just like light.
For the record, light has no effect on colonizing mycelium, good or bad. The old advice of "incubate in total darkness" is bunk. Those words were written by Stamets in TMC 20 years ago, and he disavows that advice today. I concur. The only real time that keeping in the dark has an advantage in my experience is during casing run, when the introduction of light after casing colonization can serve as one of the pinning triggers along with air exchange and proper humidity. Bear in mind, you want a constant rate of evaporation from your substrate to acheive the best pinset. If you're at 100% humidity, there will be little to no moisture evaporating from your casing layer, and pinsets will suffer.
To repeat, light is a pinning trigger, but it isn't the only one, and it's greatly overrated. For the best pinsets, you have to balance several triggers at once. Screw up on any of them, and pinsets suffer, regardless of what you do with light. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Less light = More pins? [Re: MLBjammer]
#5593326 - 05/05/06 09:25 AM (17 years, 8 months ago) |
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you changed the rules on us a little here between this and your last post if i remember right i thought you meant you kept your other one in complete darkness. now i see you mean that you've switched to darkness once you got pins. you also said it grew bigger shrooms, not more pins...
there might be an explanation for you now though. perhaps when the lighting is stopped, the formation of new pins is stopped (or at least slowed), and more energy is focused towards the existing ones, causing them to grow out better, abort less, etc. even though growth shows distinct flushes, i'm sure you realize its not exactly even. not all pins arrive at the same identical moment. your second flush pins are usually starting to develop as the first flush is reaching maturity. so maybe taking it out of light "pauses" the pinning process, and all the resources get devoted to whats been started resulting in healthier shrooms.
this is just my theory... don't anybody mistake it for fact do you notice more than usual delay between flushes with the ones grown in darkness? if so, that would jive with my explanation. makes intuitive sense though, and if you keep getting good results repeatedly that way, maybe you're on to something!
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Less light = More pins? [Re: RogerRabbit]
#5593742 - 05/05/06 11:39 AM (17 years, 8 months ago) |
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Quote:
RogerRabbit said: Many things. I've found the brightest light stimulates more pins. You need to look at pinning triggers like the instruments in a band. One instrument can be slightly off, and the band still plays the song. Often one instrument can be taken away and the music still sounds ok. However, if all are working together, it's awesome.
Perhaps the projects you remove from light after exposure are mainaining a higher humidity due to no heat from the lights. Humidity is a pinning trigger just like light. Maybe the ones you remove from the lights get better air circulation. Fresh air is a pinning trigger just like light.
For the record, light has no effect on colonizing mycelium, good or bad. The old advice of "incubate in total darkness" is bunk. Those words were written by Stamets in TMC 20 years ago, and he disavows that advice today. I concur. The only real time that keeping in the dark has an advantage in my experience is during casing run, when the introduction of light after casing colonization can serve as one of the pinning triggers along with air exchange and proper humidity. Bear in mind, you want a constant rate of evaporation from your substrate to acheive the best pinset. If you're at 100% humidity, there will be little to no moisture evaporating from your casing layer, and pinsets will suffer.
To repeat, light is a pinning trigger, but it isn't the only one, and it's greatly overrated. For the best pinsets, you have to balance several triggers at once. Screw up on any of them, and pinsets suffer, regardless of what you do with light. RR
My thoughts exactly. Incubating in total darkness makes the use of the light trigger much more effective. It is the coincidence of all these triggers at the exact same time that gives one the most bang for the buck!
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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