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OfflineSaintAK
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Registered: 04/23/06
Posts: 13
Last seen: 17 years, 4 months
Sterilizing Using PC and Possible Overcooking?
    #5586986 - 05/03/06 06:08 PM (17 years, 8 months ago)

I am doing the PF Tek method. I have already PC'd the jars for 1 hour at 15 psi. I've read in another forum that just to be safe people were pc'ing for 1 1/2 hours.

If I sterilize them in the PC for another 1 1/2 hours will I be overcooking the jars? Also, when the water contents begin to boil will that create steam inside the jar, then it could leak out from under the tin foil? I have medical clothe tape covering the four holes. Advice would be much appreciated.


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OfflineSnaggletooth
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Registered: 10/24/05
Posts: 6,109
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Re: Sterilizing Using PC and Possible Overcooking? [Re: SaintAK]
    #5587009 - 05/03/06 06:11 PM (17 years, 8 months ago)

1hr for PF is fine.

When people go over it's usual cause they are filling the PC and or have big bags or large jars.

Otherwise no need.

You dont want the jars to be sealed, then it will create a vacuum and when you use them they will suck your syringe dry and or suck in dirty air.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Sterilizing Using PC and Possible Overcooking? [Re: Snaggletooth]
    #5587110 - 05/03/06 06:29 PM (17 years, 8 months ago)

At 15psi, your substrate only needs 15 minutes to sterilize.  We use the one hour thing as a safety because it takes time for the heat to penetrate to the center of the jars.  One hour should be plenty for pf jars, but if you move up to fp bags, you might need to go as long as four hours to get the core temps of the bags up to 250 for over 15 mins.

Don't worry, you should be fine
:bigjoint:


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Offlinethenewguy05
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Re: Sterilizing Using PC and Possible Overcooking? [Re: monstermitch]
    #5587136 - 05/03/06 06:34 PM (17 years, 8 months ago)

1 hour is fine. for grain i use 90 minutes but that is because they contain many endospores and you want the steam to penetrate every nook and cranny.

for liquid culture only 20 minutes is good because you CAN overcook those.


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OfflineSaintAK
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Registered: 04/23/06
Posts: 13
Last seen: 17 years, 4 months
Re: Sterilizing Using PC and Possible Overcooking? [Re: thenewguy05]
    #5587577 - 05/03/06 08:14 PM (17 years, 8 months ago)

Thanks for the advice.


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