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Offlinepocketsand
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One inoculation spot?
    #5583660 - 05/02/06 10:21 PM (17 years, 8 months ago)

This is a somewhat strange question. I inoculated one of my brazilian 1/2 pint jars in four spots, but for some reason there is mycelia growth at only one point. How long might this take to fully colonize my jar? Standard PF tek by the way


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OfflineSnaggletooth
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Re: One inoculation spot? [Re: pocketsand]
    #5583706 - 05/02/06 10:29 PM (17 years, 8 months ago)

The others might be fine, they dont grow at the same rate, I would say just wait it out.

Not every cc contains the same amount of spores.


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Invisiblemusher_420
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Re: One inoculation spot? [Re: pocketsand]
    #5583737 - 05/02/06 10:33 PM (17 years, 8 months ago)

You can better distribute spores in your syringe by shaking it well. If you make your own a few drops of jet dry in the solution will help keep the spores in suspension.


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Offlinepocketsand
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Re: One inoculation spot? [Re: musher_420]
    #5583917 - 05/02/06 10:56 PM (17 years, 8 months ago)

its been about two weeks though and its happened in multiple jars. The syringe i bought came from a friend of mine and he accidently kept it in his car on a hot day for about an hour or two so that might have killed a few of the spores. I inoculated seven jars and not one of them has colonization in all four spots. So how long do you think it would take with just one portion colonizing?


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Offlinefuturewolf42o
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Re: One inoculation spot? [Re: pocketsand]
    #5583929 - 05/02/06 10:58 PM (17 years, 8 months ago)

if certain innoc spots have not colonized, you might consider shaking the jars, although if sterility was observed, waiting for the colonized myc to spread is always an option.


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Offlineskeletor
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Re: One inoculation spot? [Re: futurewolf42o]
    #5584304 - 05/03/06 12:30 AM (17 years, 8 months ago)

nah you'll be fine might take a month though just under. i did up some shitty brf jars, shitty as in i didn't pay attention to my mix, so i could test some lc's they have taken 3 weeks and are just finishing. i htink i'll put them outside. im not sure if its warm enough yet, guess we'll see.


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Offlinepocketsand
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Re: One inoculation spot? [Re: skeletor]
    #5584818 - 05/03/06 08:37 AM (17 years, 8 months ago)

thanks everybody


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