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InvisibleBlimeyGrimey
Collector of Spores
Male

Folding@home Statistics
Registered: 08/24/05
Posts: 3,788
Loc: Puget Sound
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: Herbus]
    #6040025 - 09/07/06 09:38 PM (17 years, 4 months ago)

k here's an update on this project.
firstly, my spore collection has been trashed, long story but putting your prints in a small lockbox is a bad idea, especially when someone steals that box (probably thought it had money in it, and i think i know who took it).

anyways, i now have the funds to order the EF kit to start this work. I have malabar spores to start with, but i believe this wouldnt be a good baseline to start from. i plan on using EQ or Gulf Coast as a starting point for comparison. i will be comparing the first sample to the PF Redspore (2nd sample) because i believe there is a good chance of noticing a difference between the two.
i'll post some more info one the kit gets here and i begin the work.
i do however need spores and some help researching the best enzyme(s) to use for spores. as stated before i want this research to be published and i dont want any legal trouble so live samples are out of the question.

i have however decided to also do a few different species (p. stuntzii, p. cyan, and p. azure come to mind) just for fun.

i will foot the financial end of this project (the EF work), but once again i'd greatly appreciate anyone who would like to donate spores or help with research.

anyways,
lets get this ball on the roll.


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OfflineNo_Life_G33k
Now with 10%less noobness
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Registered: 03/08/05
Posts: 356
Last seen: 13 years, 10 months
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: BlimeyGrimey]
    #6047578 - 09/10/06 06:13 PM (17 years, 4 months ago)

I'm also wondering about the variation to be found within one 'strain'... potency/yield etc.... I think we really need more examination of this area.


--------------------
Hot Water Pasteuriztion - A How To Pictorial
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4527808/an/0/page/0

Make your own shroom chocolates
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4500682/an/0/page/5

guide with pix.


Make Your Own Magnetic Stirrer! How-To Tek w/ pix
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5865949/an/0/page/3


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Invisiblefastfred
Old Hand
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Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: No_Life_G33k]
    #6048959 - 09/11/06 02:47 AM (17 years, 4 months ago)

Awesome to see this thing get going. As far as enzymes... I would just do a few searches on what has already been used and pick one of those. There has already been a ton of RFLP work done on various fungi.

It should be a pretty easy task to choose some good candidates. If you need any help feel free to PM me. You really can't go wrong here. EcoR1 and BamH1 are the most common restriction enzymes that would be on hand, so I would be tempted to use those and would be surprised if they weren't the ones in common use for RPLP.


-FF


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OfflinePaulyPoor
Stranger
Male

Registered: 07/02/06
Posts: 12
Loc: Southern Illinois
Last seen: 16 years, 10 months
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: fastfred]
    #6049995 - 09/11/06 01:34 PM (17 years, 4 months ago)

this is an interesting idea... sounds like alot of people have access to labs... excellent, i myself work in a phytochemistry lab... i don't remember who it was who said it, because i only have a limited amount of time to do this, i'm a busy man these days, which is why i am probably a stranger to all of you, but anyways, i think that the variation will be much much much less than 25%, most genetic variance between related species is very minute let alone mr's of the same species but different races? anyways i think we have the beginnings of an interesting line of inquiry here


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OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
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Registered: 06/18/02
Posts: 1,806
Loc: Land of Oz
Last seen: 5 years, 8 months
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: PaulyPoor]
    #6055211 - 09/12/06 09:09 PM (17 years, 4 months ago)

Hey BliemyGrimey - I sorted out things with my lecturer and I can also do an RFLP; so we can combine our results.

I will carry out RFLP's between the members of the Psilocybe genus - and you already look set to do comparisons between cubensis strains so we have both bases covered!
I already have a reasonable (but small) selection of different Psilocybe species, so I'll get right onto sourcing some of the others... :laugh:


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OfflineIGNVS
Stranger

Registered: 04/23/10
Posts: 17
Last seen: 13 years, 1 month
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: Feelers]
    #13548152 - 11/27/10 01:08 PM (13 years, 2 months ago)

did anything ever come of this?


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OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
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Registered: 06/18/02
Posts: 1,806
Loc: Land of Oz
Last seen: 5 years, 8 months
Re: The Shroomery Strain DNA-Length Analysis Project! [Re: IGNVS]
    #13551758 - 11/28/10 08:54 AM (13 years, 2 months ago)

Wow it's been a while since I posted in this thread. In the end Sporeworks came through (very generously) and sponsored me with many spore prints to try out. I tried to actually get the Large ribosomal subunits sequenced, but I was told that it was too expensive by my lecturer.

To do all the species I had was going to cost around $600, the budget for the entire class was $1200. I asked him how much of that money had really been spent and he said less than $200, but that it wasn't fair to the other students. I eventually offered to pay for it myself, but he said I didn't know how to use the sequencer/pcr equipment. When I found a PhD student who was going to help me, the lecturer was still dead set against it.

Funny because I now have my own PCR!

All was not lost however, I revamped my experiment to utilize other expensive things that could be done in the time remaining, and jumped on the electron microscope.
These threads...
Here...
And here...



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