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Ascension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
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Was it substrate or from the needle?
#5582946 - 05/02/06 08:19 PM (17 years, 8 months ago) |
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I inoculated this jar 4 days ago with just a basic spore syringe from a print I had made. The substrate was PC'd and the needle was cleaned, and im pretty sure the print was clean. (took all precatutions to make a clean print) But obviously somewhere something went wrong as their is green mold.

PS, sorry bout the bad pic, it was taken with my phone. It looks kind of black but trust me the mold is very green.
Anyways if you look at the picture, you can see the green is ONLY where the myc is growing.
Now my question is, does the green meanie only show itself where the myc is growing, because im thinking if the substrate wasnt sterelized properly then the green would be everywhere, but since its ONLY where the myc ha started to grow, then it must of come in WITH the spores, so is this good enough deductive reasing to say the contams came in from the needle?
Or does the green only like to show up once the myc has shown? because if thats the case then my substrate may have not be sterelized properly.
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MAIA
World-BridgerKartikeya (DftS)


Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
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Re: Was it substrate or from the needle? [Re: Ascension]
#5585212 - 05/03/06 10:47 AM (17 years, 8 months ago) |
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I wish things were that simple man. To know if the problem is from the syringe, you'll need to have a control jar on the next batch. Just don't inoculate one jar and see if it gets infected as well.
Besides the substrate and the syringe, the inoculation procedure is also very important. So first, what substrate did you use, what PCing timings were applied and how did you inoculate ?
MAIA
-------------------- Spiritual being, living a human experience ... The Shroomery Mandala
 Use, do not abuse; neither abstinence nor excess ever renders man happy. Voltaire
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Ascension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
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Re: Was it substrate or from the needle? [Re: MAIA]
#5588610 - 05/04/06 12:27 AM (17 years, 8 months ago) |
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substrare is brf/verm. PC'd for an hour. I inoculated by taking a clean syringe and cleaning the outside of a baggie that had a spore print inside on a peice of cardbord. Injected the water, and got all the spores into solution then sucked back into the syringe. Used rubbing alcohol to clean everything and flamed the tip, all precautions were taken.
I have 3 other jars that seem fine so maybe it was just bad luck.
If the other 3 jars are okai, and they were injected with the same syringe then I guess it would have to be the substrate, actually that was pretty obvious then.
But do you understand what I was trying to say? the mold ONLY appeared where the myc has started growing, so if the jar was contamed then it must of been pot luck that the green started at the same point the myc did. UNLESS the contam came in with the spore solution then the mold could ONLY start where the myc starts to grow.
If the myc started growing on one side of the jar, and the green started on the other side, then the substrate must of been the cause.
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MLBjammer
Invitro Freak


Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
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Re: Was it substrate or from the needle? [Re: Ascension]
#5589203 - 05/04/06 07:20 AM (17 years, 8 months ago) |
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You could PC some jars and leave them for a few days as a test. If they still look good after 3 days or so then you know it was your syringe.
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MagicalKnife
Shroomologist


Registered: 02/12/06
Posts: 135
Last seen: 15 years, 5 months
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Re: Was it substrate or from the needle? [Re: MLBjammer]
#5590606 - 05/04/06 04:15 PM (17 years, 8 months ago) |
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Someone said the Green Meanie has mycelium similar to the mycelium of the mushroom and that the green parts are actually the spores of the mould. Who knows, half of that mycelium might not even be mushroom mycelium.
-------------------- ?
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RogerRabbit
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Registered: 03/26/03
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Re: Was it substrate or from the needle? [Re: MagicalKnife]
#5590628 - 05/04/06 04:24 PM (17 years, 8 months ago) |
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Trichoderma spores are killed by very low temperatures. In fact, they're killed off by temperatures far below boiling. We don't use a pressure cooker to avoid green molds. Boiling alone would do that. We use a pc against bacteria. If you followed even basic PC instructions, you can be sure you introduced the mold when you inoculated. Either your print, the syringe, the water or the needle were dirty, or all of the above. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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