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 Catzeye
member
Registered: 10/24/01
Posts: 168
Loc: The South
Last seen: 22 years, 1 month
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 Clonning Mushrooms by injecting H2O into a shroom. 
#557500 - 02/20/02 01:45 PM (23 years, 1 month ago) |
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I read a post a few months back about someone who had cloned a shroom by inserting a hypodermic needle into the cap of a shroom and injecting sterile water then sucking it back in.
Has anywone tried this technique? Sounds Fascinating to me.
Catzeye
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~It is Green, It Is AquaMarine~
It is Colors I have Never Seen.
I can See Past You
To the White Sands.
Stevie Nicks
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Azure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 10 months
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 Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
#558052 - 02/20/02 11:53 PM (23 years, 1 month ago) |
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It doesn't sound like you'll have consistent results because you can't guarantee the sterility inside the mushroom. However, if you clone a piece of tissue on peroxidated agar, it doesn't really matter whether it's sterile or not, so that increases your chance of sucess.
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 Zen Peddler
 
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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 Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Azure]
#558073 - 02/21/02 12:16 AM (23 years, 1 month ago) |
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Ive had recent success by taking pieces of a fruit from the interior of its stem, grinding this into a glass of h202, then placing it straight from this glass - using a glovebox - into a sterile sealed dish of either malt/water (standard 1ml malt to 100mls water) or as Lizardking suggests a brf and water paste.
As soon as i witnessed growth, i hit it with about 2.5mls of h202 waited 24 hours, then used this liquid as innoculatant. Worked great for me. Ill post pics very soon.
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mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
#558297 - 02/21/02 08:31 AM (23 years, 1 month ago) |
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I think what you are referring to is injecting the water near the gills before the veil separates, right? Basically you'll get a few spores, not mycelium. They will almost always be sterile, unless you see a contam directly attacking the subject fruit body. And you aren't cloning, you are just getting sterile spores.
The practice works rather well, although not 100% consistency. Sometimes it just seems like there aren't enough spores that early on. Could also be strain dependent.
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"From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Catzeye
member
Registered: 10/24/01
Posts: 168
Loc: The South
Last seen: 22 years, 1 month
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Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: mycofile]
#558566 - 02/21/02 01:05 PM (23 years, 1 month ago) |
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Well, if memory serves me correctly. Someone had tried sticking a syringe full of water into a living mushroom, injecting the water and sucking it back in. From what I can remember (the brain is not what it used to be) the needle was inserted into the point of the cap and pushed down into the area where the stem and cap meet. Yes, there would be sterility questions. I was just wondering if any of you had tried such a method. I guess the ideal would be to retrieve dikaryotic mycelium. Of course agar or malt plates with peroxide and transfering a portion of the shroom would be more effective. This other way would be quicker if you could get viable mycelium into the syringe. I guess you could even mix the syringe water with peroxide to kill bacteria. I may have to try this sometime as an experiment. Anybody else game?
Catzeye
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~It is Green, It Is AquaMarine~
It is Colors I have Never Seen.
I can See Past You
To the White Sands.
Stevie Nicks
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mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
#560040 - 02/22/02 05:31 PM (23 years, 1 month ago) |
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I just don't think you are going to get much/any mycelium. Then again, perhaps the needle will extract a plug of mycelium which could be sucked into the syringe. In that case, no need to inject water, just use a half full syringe and try to suck the plug through into the syringe. Doesn't sound promising though.
Again, the only similar method I've ever heard of or tried is extracting small amounts of spores from the cap before it opens, thus ensuring sterility.
--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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