Home | Community | Message Board


Gaiana.nl
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Brown Rice Flour

Jump to first unread post. Pages: 1
OfflineCatzeye
member

Registered: 10/24/01
Posts: 168
Loc: The South
Last seen: 14 years, 3 months
Clonning Mushrooms by injecting H2O into a shroom.
    #557500 - 02/20/02 03:45 PM (15 years, 3 months ago)

I read a post a few months back about someone who had cloned a shroom by inserting a hypodermic needle into the cap of a shroom and injecting sterile water then sucking it back in.
Has anywone tried this technique? Sounds Fascinating to me.

Catzeye


--------------------
~It is Green, It Is AquaMarine~
It is Colors I have Never Seen.
I can See Past You
To the White Sands.
Stevie Nicks


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 15 years, 29 days
Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
    #558052 - 02/21/02 01:53 AM (15 years, 3 months ago)

It doesn't sound like you'll have consistent results because you can't guarantee the sterility inside the mushroom. However, if you clone a piece of tissue on peroxidated agar, it doesn't really matter whether it's sterile or not, so that increases your chance of sucess.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Azure]
    #558073 - 02/21/02 02:16 AM (15 years, 3 months ago)

Ive had recent success by taking pieces of a fruit from the interior of its stem, grinding this into a glass of h202, then placing it straight from this glass - using a glovebox - into a sterile sealed dish of either malt/water (standard 1ml malt to 100mls water) or as Lizardking suggests a brf and water paste.
As soon as i witnessed growth, i hit it with about 2.5mls of h202 waited 24 hours, then used this liquid as innoculatant. Worked great for me. Ill post pics very soon.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
    #558297 - 02/21/02 10:31 AM (15 years, 3 months ago)

I think what you are referring to is injecting the water near the gills before the veil separates, right? Basically you'll get a few spores, not mycelium. They will almost always be sterile, unless you see a contam directly attacking the subject fruit body. And you aren't cloning, you are just getting sterile spores.

The practice works rather well, although not 100% consistency. Sometimes it just seems like there aren't enough spores that early on. Could also be strain dependent.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCatzeye
member

Registered: 10/24/01
Posts: 168
Loc: The South
Last seen: 14 years, 3 months
Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: mycofile]
    #558566 - 02/21/02 03:05 PM (15 years, 3 months ago)

Well, if memory serves me correctly. Someone had tried sticking a syringe full of water into a living mushroom, injecting the water and sucking it back in. From what I can remember (the brain is not what it used to be) the needle was inserted into the point of the cap and pushed down into the area where the stem and cap meet. Yes, there would be sterility questions. I was just wondering if any of you had tried such a method. I guess the ideal would be to retrieve dikaryotic mycelium. Of course agar or malt plates with peroxide and transfering a portion of the shroom would be more effective. This other way would be quicker if you could get viable mycelium into the syringe. I guess you could even mix the syringe water with peroxide to kill bacteria. I may have to try this sometime as an experiment. Anybody else game?

Catzeye


--------------------
~It is Green, It Is AquaMarine~
It is Colors I have Never Seen.
I can See Past You
To the White Sands.
Stevie Nicks


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Clonning Mushrooms by injecting H2O into a shroom. [Re: Catzeye]
    #560040 - 02/22/02 07:31 PM (15 years, 3 months ago)

I just don't think you are going to get much/any mycelium. Then again, perhaps the needle will extract a plug of mycelium which could be sucked into the syringe. In that case, no need to inject water, just use a half full syringe and try to suck the plug through into the syringe. Doesn't sound promising though.

Again, the only similar method I've ever heard of or tried is extracting small amounts of spores from the cap before it opens, thus ensuring sterility.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop:   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Brown Rice Flour

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Mushroom cultivators - the Shroom needs you! AsanteA 1,413 12 03/08/13 01:18 PM
by Javadog
* different colored shrooms? crazytab 1,085 3 07/27/03 09:34 PM
by resin
* Re: Mycelium H2O + H2O2? joee 2,222 6 11/30/99 06:07 PM
by mycofile
* "Hydraponic Mushroom Growing"
( 1 2 all )
shroomballa 3,810 33 09/07/12 01:01 PM
by RogerRabbit
* Mushroom Storage - C02 shroomologist 1,530 15 12/18/02 07:02 AM
by Raadt
* Re: WANTED: Your input on co2 injection into grow chamber Code 1,388 6 01/26/00 06:08 PM
by PanTrop
* exploding mushrooms Fick_Duck 1,723 16 11/13/02 08:04 PM
by mycofile
* psychedelic mushrooms and developing nations - a new chapter in the relation between shrooms and man AsanteA 1,048 4 02/01/06 07:01 AM
by blackout

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit
1,102 topic views. 1 members, 4 guests and 3 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Sporeworks
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.007 seconds on 18 queries.