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creamcorn
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Posts: 2,962
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Tweaking PF jars for super fast colonization! 6
#5574833 - 04/30/06 06:00 PM (17 years, 8 months ago) |
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Ok. All the newbies ask it. "How can I speed this up?" Well, here you go. This isn't a tek per se, its more of a collection of tips that even a first timer can use, although it does assume you're very familiar with the pf-tek method, and preferably have gone through it at least once... using all these tips should get you similar results. Using some of them will still help improve things!
Phase 1: Jar preparation - Pack your jars loosely kids. That can't be stressed enough. Spoon your mix in and absolutely do not pack it down one bit. Inspect your jar before putting the dry verm layer on top. If you see a big gap somewhere, or a clump of BRF or otherwised packed section, dump it back into your mixing bowl and fill it again so it looks consistent. An extra minute or two effort here can save you days. - Use wide mouth jars if possible. The bottom, which is usually most stubborn to colonize, gets started much faster. Your needle reaches perfectly to the half-way point, rather than near the top too. - Try 5 holes in a wide mouth! It works wonders. Space them evenly. Draw a star on top if you want to help visualize where to put your holes. As you can see in the picture I didn't do a very good job at that on this jar Its not critical though. - Use coffee! It is like rocket fuel for BRF jars. Its tried and tested with grains, but you can use it in a BRF jar too. 25% freshly brewed coffee to 75% water works quite well. Tip: There's 4 tablespoons in a quarter cup, so on a per-jar level thats a tablespoon of coffee per three tablespoons of water. I'm sure you folks are smart enough to figure out how to get that ratio in larger measurements. - Bake your ingredients first. Lay out the BRF in a pyrex dish or baking pan, as well as a whole bunch of verm in another. Bake at 275 for about 30 minutes. Two purposes here: the main idea is to bake the moisture out. Verm out of the bag is already damp. BRF is to an extent too. Moreso once the packages have been opened and they have sucked moisture out of the air. This way you know every time you're starting with an identical baseline in dryness with your ingredients. The second purpose, it gives you a head start on sterilizing. You know your ingredients are clean when you start. Please don't misconstrue this as a means to skimp on your boiling or pressure cooking. You still must sterilize as normal once the jars are prepared.
Phase 2: inoculation - Use liquid culture. Learn it and love it. Its easy even for newbies, don't be intimidated by it. Its the most efficient way to use spores, and shaves time off like you wouldn't believe. - Make sure to get as much mycelium from your liquid culture into your syringe as possible! The more the merrier! Whats left in your LC jar will grow back if the nutes aren't spent. - Be generous with the LC when innoculating. 2.5CC per jar is great. Divide the amount out by your holes to figure it out. That's .5CC per hole when using 5 holes. You'll need about 3 syringes full to do a dozen jars. A fantastic thing happens when using this much: it drips all the way down to the bottom. Colonization starts in stripes across the jar reaching around the bottom right away, rather than little round spots like with spores.
Phase 3: Incubating - For the love of god, use a proper tub-in-tub incubator. This is the best $25 dollar and 15 minute investment you could possibly make in this hobby. Hold jars at a steady 82-84F. - Get that damn tape off! I like the following method: cover jars in foil when boiling/pc'ing, no tape here. Tape after you inoculate, preferably with micropore tape (medical tape, you can find it near the band-aids). After 3-4 days when colonization really takes off, take the tape off! I like to turn it sideways, and put it back on at an angle from the ring to the lid, sort of like a "tent" over the hole. It leaves the hole totally breathable, yet acts sort of like a dust cover. See the picture for what I mean. - Try not to peek so much! I know its hard to help, especially starting out. It doesn't have anything to do with letting light in, but everything to do with keeping your heat in and steady. Every time you open up your TiT you let heat out and it takes time to build back up.
Here's some pictures for you. This bad boy is a B+ that was inoculated on the 24th, that's a mere 6 days ago. Some of you know when starting from spores at 6 days you're lucky to see tiny little fuzz balls starting. Should be done by day 10 if not sooner! The proof is in the pictures here that these tips work:
Side view:

Bottom view:

Top view (note 5 holes, and the little "tape tent" method I explained, the holes are underneath, but they're not actually covered since the tape isn't stuck down onto them):

Happy growing!
Edited by creamcorn (04/30/06 06:43 PM)
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WIZOLZ
Poor with Needs


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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5574859 - 04/30/06 06:14 PM (17 years, 8 months ago) |
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Your one helpfull fella CC...These tips are an excellent collection of things to take into consideration, if not follow exactly when working with PF projects. I havnt seen that baking step before when preping the Jar mix, although, I actually did do this when preparing my first PF jars about a month ago. What surprised me was how quickly it obsorbed the water when it was added and how much it fluffed out compared to just regular BRF and Verm. If I could get a 1/2 pint to colonize fully in 6 days, that would be great! I'll have to go with that coffee tek next time, sounds beneficial for sure. Of coarse, by that time I'll have the LC ready. (Started today)
Zooooooooooom Zoooooooom
Edited by WIZOLZ (04/30/06 06:21 PM)
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monstermitch
Growing in Bags Doesn't Work


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Re: Tweaking PF jars for super fast colonization! [Re: WIZOLZ]
#5574948 - 04/30/06 06:54 PM (17 years, 8 months ago) |
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Great collection of tips for brf jars, and well explained.
I have some brf sitting around from a while ago, I'll give it a go step for step your way. I have some stevia, algit, and black mustard seed that I use in other methods. Do you see any benefit to using additives such as these?
With no h/poo, I figure the substrate could use some help in the nute dept.
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creamcorn
mad scientist


Registered: 03/13/06
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Re: Tweaking PF jars for super fast colonization! [Re: monstermitch]
#5574979 - 04/30/06 07:03 PM (17 years, 8 months ago) |
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no direct experience with additives besides coffee in brf jars to give a good answer, but i don't see how they could hurt. one thing all those ingredients you mentioned have in common is a high protien content, specifically tryptophan. now that the fda allows sale of pure l-tryptophan as a suppliment again i've been toying with the idea of adding that to the water for a potency boost. we've been discussing it quite a bit in this thread. should it work well, that will be the next step towards the easy preparation of the ultimate pf jars i certainly won't reccomend such things until its been tested and proven though.
but i say use what you got, don't see it as causing any harm.
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monstermitch
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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5575001 - 04/30/06 07:10 PM (17 years, 8 months ago) |
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thank you creamcorn.
I have read the thread and I respect what RogerRabbit has done with his efforts. He attests to no real evidence in potency gain, so I place no merit in it. And yes, I know they are all high in protein and tryptophan, that's why I got them.
Yeah, I'll use them, and now the coffee too. Sounds great. the available tryptophan I can find is really pricey though. Hope someone will track down some cheaper. I'll keep my eye on that thread too.
Thanks again creamcorn.
Just was wondering
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creamcorn
mad scientist


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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn] 1
#5703822 - 06/02/06 11:51 AM (17 years, 7 months ago) |
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just a little bump here for those who haven't seen this. i've also gotten a lot of feedback (all good!) and some questions on the coffee aspects, so i'll clear those up and give you some mycoporn inspiration!
first off, the coffee formula isn't very sensitive. don't worry about how exact you measure it. don't worry how strong or weak you brew your coffee, just brew it like you'd normally drink it and that's "close enough"... don't stress on brands of coffee or quality, it really doesn't matter. i'm beginning to suspect its the high nitrogen content, along with other nutrition in coffee that's playing a part here. you can probably use decaf with exact same results. there's more coffee related experiments on the way, stay tuned. (a case in progress right now, a couple crumbled cakes spawned to a pastuerized coir/coffee ground mixture, should see pins there shortly and will post on it...)
well i'm totally convinced coffee is awesome for colonization speed. but people have also asked how the fruiting is.
on average the cakes with coffee pin in about 5 days after a dunk. this regularly beats out non-coffee cakes by 2-3 days. i dunk 12hr and double end case (verm on top, tray of moist verm on bottom). i occasionally "feed" the tray of verm on the bottom with water from an eyedropper, maybe two or three times throughout the fruiting cycle.
potency as far as i've observed so far is as good if not slightly better than straight brf. haven't had time to eat all these guys yet to come to a real conclusion there. 
these pictures are from the same batch of jars the example jar in the first post was from. its been so long because they've been hanging out in the fridge a while waiting for room. my jars colonize so fast these days it takes much longer to fruit. especially because i *always* get 3 good flushes, and have gotten as many as 6!
first flush, intense pinsets!

cake on its second flush, not so shabby eh?

third flush, only get 3-4 shrooms off a cake, but they're monsterous and tend to outweigh an abundant flush of smaller ones. here's two 30+ gram specimens that are absolutely gorgeous. not a bad size to be regularly pulling from a cake, huh?

keep the questions, comments, and success stories coming, i love it
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IGnosticAbhorI
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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5703886 - 06/02/06 12:15 PM (17 years, 7 months ago) |
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I wouldn't attribute everything to the coffee.
I remeber when RR first posted about it in Adv. Cult. way back when and everything was rather inconclusive.
IME, I get about the same results...Although it's rather difficult to get scientific results since lots of peeps don't have controls and such for their projects. However, it doesn't seem to harm anything (coffee), so 
Nice fruits BTW 
I wonder if RR or anyone else has tried casings utilyzing coffee...As in dunking the cakes in coffee solution before breaking them up and spawning or casing....
-Gnostic
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creamcorn
mad scientist


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Re: Tweaking PF jars for super fast colonization! [Re: IGnosticAbhorI]
#5703907 - 06/02/06 12:21 PM (17 years, 7 months ago) |
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well I confidently attribute it to the coffee. its not huge, but its noticable. consistently faster colonizing cakes, quicker to pin, and more prolific fruiting, compared side by side to cakes of the same strain in the same FC grown literally side by side. this is after many many dozens of cakes, not just the ones i'm showing here... these were just handy examples.
i have closely studied those original thread(s) about the coffee and you're right, there certainly weren't conclusive results. but we're talking a little different.. that was mostly in reference to soaking grains in coffee water, here i'm using standard pf cakes, substituting plain water with a coffee solution... i don't recall anything from those threads about anybody trying it this way. i've experimented with many different concentrations of coffee water too to get where it is today at the 25% reccomendation. coffee (spent grounds) has long been used in commercial cultivation, so i think its usefulness is long known yet often overlooked.
take it as you will, but i garuntee you won't see any harm done, and have a hunch you'll be pleased with the results you do get
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WIZOLZ
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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5706435 - 06/03/06 01:19 AM (17 years, 7 months ago) |
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Good to see your still adding to this thread CC. I also much enjoy the experimentation factor involved with cultivation, though, I must admit, I'm still an amateur at this point. None the less, its fair to say that although mycelium prefer these well documented grow mediums (BRF, RYE, HPOO etc.) it is not entirely restricted to them. I used a 1/8 cup of orange juice in a BRF cake and though it didnt colonize any quicker, or more efficiantly (lots of mico-piss) it still validated the ability to fruit, which it is now in a small OJ contanier...^_^
God love these little mushrooms...
-------------------- ---------o----o----o-------o------------------------o--o-o- ---------------------------------------------------------------- Requim for a Dream - Paul Oakenfold --------------------------------------------------------------- "The mis/abuse of any form of power, is the worst form of ignorance" ------------------------------------------------------------- WIZOLZ - Lover with a Killer's Smile
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kilroy
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Re: Tweaking PF jars for super fast colonization! [Re: WIZOLZ]
#5706466 - 06/03/06 01:30 AM (17 years, 7 months ago) |
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Ever since I found this thread I have been using coffee in my brf jars and I must admit it helps the colonization alot. Thanks CC and I usally include a link to this thread when new people ask questions about speeding up thier jars. GJ keep it up
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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IGnosticAbhorI
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Re: Tweaking PF jars for super fast colonization! [Re: kilroy]
#5706703 - 06/03/06 04:53 AM (17 years, 7 months ago) |
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Indeed.
What bugs me most is knowing exactly what it is in the coffee (if it's anything at all)
Caffinee? Something in the bean itself? That's what drives me nuts.
However, without a scientific study with controls and certains things mointored closly and without using an isolated strain, you can't say for a fact that it's due to coffee. However, you can't say that it isn't due to the coffee either 
Either way ...Great thread regardless
-Gnostic
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creamcorn
mad scientist


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Re: Tweaking PF jars for super fast colonization! [Re: IGnosticAbhorI]
#5719156 - 06/06/06 02:27 PM (17 years, 7 months ago) |
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Quote:
IGnosticAbhorI said: Indeed.
What bugs me most is knowing exactly what it is in the coffee (if it's anything at all)
For starters coffee has a high nitrogen content. This is why it's an awesome additive to compost used for mushroom growing, and why its used as plant fertilizer too. A high nitrogen content in hpoo is one of the reasons it works so well. Check this too, coffee has an array of amino acids, vitamins and minerals. We don't think of coffee itself as being very nutritive - but there was a study released recently that most Americans get a majority of their antioxidants from coffee! It's actually rather nutritionally diverse and even though there's certainly things in it useless to a mushroom, there's things useful to it (like phosphorus, which is an all important element when creating psilocybin.)
Quote:
Caffinee? Something in the bean itself? That's what drives me nuts.
Totally doubt its the caffeine. I've been meaning to put that one to rest as I've got a literal "jug" full of lab grade caffeine anyhdrous powder sitting around the house I could certainly experiment with.
Quote:
However, without a scientific study with controls and certains things mointored closly and without using an isolated strain, you can't say for a fact that it's due to coffee. However, you can't say that it isn't due to the coffee either 
Excellent point. That's why that is pretty much how I did it - a tissue culture grown out in LC, and used to start many jars with varied amounts of coffee and controls with none at all. I'm totally dorking and assign a "tracking number" of sorts to my jars and keep a little log book so I know exactly what has what in it, which cake is which, etc. Results have been coming in and continue to do so; got a stockpile of 'em in the fridge and I run a control example along side some of the coffee ones and have repeated a few times, am going for the 4th try at the moment and am pretty confident its going to agree with the first three runs through I wasn't satisfied with the lack of conclusion in previous threads so I did my homework.
Quote:
Either way ...Great thread regardless -Gnostic
Thanks. There's more to come.
I'm working on figuring out "how much is too much"... coffee grounds, if left as grounds, are an excellent source of slower releasing nitrogen. We want our N levels around 2.7% (I forget where I read this but I know I've read it!) I'm trying to figure out how exactly to tweak a regular old PF substrate to have something in that neighborhood by actually adding spent coffee grounds right into the mix. A few early experiments so far show myc gobbles it down at alarming rates while colonizing. Problem is its also acidic, and while myc doesn't seem to mind, bacteria also love it - I can tell you if you want to lose 70% of your jars to bacteria, go ahead and add coffee grounds to them. So part of the process here next is figuring out a pH buffer (thinking gypsum) and doing some pH testing to come up with the right amounts to suggest an easy-to-follow recipe.
Should be interesting. Again its like all the discussions with all the tons of possible additives out there, and this is no miracle pill, but I think this one is pretty appealing because you don't have to hunt around for exotic ingredients in health food stores or anything, just grab those left over coffee grounds in the pot from your morning brew and you're on your way to happier mushrooms
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retro_killa
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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5746595 - 06/13/06 05:54 PM (17 years, 7 months ago) |
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wow i like it alot i hope mine turn out to look that good
-------------------- - Get outdoors it's amazing! - Enjoy life to the fullest \m/ d-_-b \m/ - RESPECT
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twiggedoubt
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Re: Tweaking PF jars for super fast colonization! [Re: retro_killa]
#5746984 - 06/13/06 07:32 PM (17 years, 7 months ago) |
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Too many variables to say that for sure, you would need to do every jar measured to the mg of every substance, you would have to have many control jars, you would have to measure the water/coffee's weight, you would have to measure its mass to the ul. You would have to but each individual jar in a temperture controlled environement. Not saying it isn't true, just saying it would be a waste of time trying to prove it, whatever works for you, if you think it works thats all that really matters, but it can't be written in stone that it works.
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kilroy
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Re: Tweaking PF jars for super fast colonization! [Re: twiggedoubt]
#5747002 - 06/13/06 07:35 PM (17 years, 7 months ago) |
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The coffee replacing a 1/4 of the water seems to really make mine go quicker.
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Tweaking PF jars for super fast colonization! [Re: twiggedoubt]
#5747111 - 06/13/06 08:00 PM (17 years, 7 months ago) |
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so you're telling me after several dozens of jars, its just a coincidence that every single one with coffee consistently outperformed every single one without (all in a semi-controlled experiment mind you... isolated clones, they were all incubated in the same incubator, they were all grown in the same FC, so they were subject to the same exact growing parameters anyway)... there's simply no need to measure minute details beyond all that.
are you familiar with statistical analysis? many things are proven every day in our world without such anal experiments. do you think they clone humans and keep them in environmentally controlled cages to test new medications? of course not. and every person is unique. yet they come to conclusions as to which medications are effective and which are harmful all the time. (haha granted i didn't DO a statistical analysis either, but i didn't have to, if you understand how they work and how "confidence intervals" are defined, you'd realize that the results i received given the number of samples didn't even merit one, the answer is pretty obvious.)
i appreciate an eye for detail but i also appreciate being realistic.
you don't have to believe me, nobody does... i've got nothing to gain by helping strangers out on some internet message board. i don't even claim credit for the idea in the first place, as it wasn't mine. so take it as you will. several folks have given it a try and noticed a marked improvement, but you don't have to believe them either. i don't think it works, i know it does, and as i finish out the last of the control jars still hanging around in the fridge i'll never do it the "old" way again
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SenselessRebel
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Re: Tweaking PF jars for super fast colonization! [Re: creamcorn]
#5914426 - 07/30/06 08:39 AM (17 years, 6 months ago) |
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i know noones been on this thread for a while but CC man you need to chill for a sec. mad props on the nice compilation of things to tweak pf jars. im definitely going to do the coffee thing. if it doesn't hurt then why not do it!!!
but on the matter of weather it helps: twigged out its right to a certain degree and so are you CC. i think twigged is going a little little bit toooo far with the measurements but he is right that if you wanna be able to 110% prove weather it helps speed up growth or not then you pretty much need to get it down to the .01 of a gram or down to the microL. and you doo need to make sure that everything stays constant except for the one variable you are testing. CC, this is in no way an attack on you and this is in no way a siding with twiggedout. all im saying is that i know the basics for scientific design. keep every variable constant in all your trials and in your control except for the var. your testing.. science runs through my fam too... my father is a shrink and has been for a damn long time. he is a professor (and does studies in many other things but i'm not trying to give away my face nor his). and if i dare say it, he's pritty famous in the shrink field,(but as a personal joke that i make with him, how famous a shrink can be hahaha) but yea and i vol. in a lab with the gov't and i dig science down to the bone. so if you do have a way to keep a constant temp in that incubator (no matter how the growth of mycelium tryes to fluctuate(sp??) it) thats one major thing cuz everyones place is different and everyones growth will change the temp differently. everyones methods are different too but ifyou get everything as exact as possible and have the only variable be the amount of coffee you got in that shit and you have several trial jars for each (at least 3) and you have the same amount of controls as trials and you prove it!!! well then damn my friend you have come across something that puts your name in the books of growing mushrooms (unlesss someone has already done a true test on this and proved it one way are another i just stumbled here today) but yea great thread and i hope you work up your self and are invested in this enough to actually do a scientific test!!!!! if you do dude and you want help setting it up by all means i'll help you and im sure there are mycologists and other scientists up in here!!! later CC -SenselessRebel
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creamcorn
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Re: Tweaking PF jars for super fast colonization! [Re: SenselessRebel]
#5915166 - 07/30/06 02:42 PM (17 years, 5 months ago) |
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Quote:
SenselessRebel said: so if you do have a way to keep a constant temp in that incubator (no matter how the growth of mycelium tryes to fluctuate(sp??) it) thats one major thing cuz everyones place is different and everyones growth will change the temp differently. everyones methods are different too but ifyou get everything as exact as possible and have the only variable be the amount of coffee you got in that shit and you have several trial jars for each (at least 3) and you have the same amount of controls as trials and you prove it!!! well then damn my friend you have come across something that puts your name in the books of growing mushrooms (unlesss someone has already done a true test on this and proved it one way are another i just stumbled here today) but yea great thread and i hope you work up your self and are invested in this enough to actually do a scientific test!!!!! if you do dude and you want help setting it up by all means i'll help you and im sure there are mycologists and other scientists up in here!!! later CC -SenselessRebel
well.... thanks for the props. but yeah the coffee thing wasn't my idea in the first place, not only has it been discussed lots on here previously, but its something commercial growers have known about for ages. so i in no way take credit for any of it, credit is due to all those who figured it out and played with it first. i just screwed around to find an appropriate mount to put in a pf cake, which by no means is a big accomplishment on its own... just a tip, on a message board, for people to try 
anyway, as far as the experimental design... you're missing the point as well as the other poster. ill just put it out there: who cares what the temps are for the experiment? they're near optimal, and relatively constant. perhaps not perfect. but they do not have to be for you to perform a valid experiment. that's what control jars are for. experimental jars AND control jars, go in the same incubator at the same exact time. so if the temp fluctuates for one category, it does so in an identical manner for the other category... because they're being treaded the same way. they were fruited side by side at the same time in the same FC too, so experienced the same temps, humidity, fae, all that.
if i split things up in some way, and tested controls first, then tested coffee second... or had two incubators to keep them separate, yeah then id have problems and it wouldn't be a valid experiment - and in fact wouldn't thave valid controls anymore. (i guess i make too big an assumption when i say "control" that people really know what it means, and all the subtleties that go with it.) thing is, i went through several rounds of this, with coffee jars and control jars, side by side every time, so they were both subject to the same conditions and things like temperature, etc can be thrown right out the window because its not significant to the outcome. if you're so interested in science, learn about good experimental design. you'll see that by doing things like this, many factors can simply be ignored with validity, because they effect the experimental and controls in the same exact way. its really the purpose of using controls. the problem with comparing things to your father's field is that, there's really no such thing as a perfect control in psychology. or in medicine, like i mentioned further back in the thread. you can give people placebos and such, but look how often the placebo recipients still 'experience' the intended result... every person is different (even identical twins come close but are still different people due to unique life experiences) so instead we use large sample sizes and statistical methods to filter out the "noise" in the experiment... its really two different ways (scientific, and statistical) to go about it, and often both types are used together. but at the end of the day, any type of experiment, can be designed to provide valid results, if you understand the nature of what you're working with.
not meaning to get off the subject too much but people keep picking at this like im trying to defend a thesis or something... maybe its my fault for not explaining how i did the experiment in the first place, but i never really thought it was all that big a deal and wasn't even the initial intention of the thread (remember, its a thread about preparing pf jars, just so happens i experimented and found the coffee to be beneficial, so i threw it in as one of the tips... was never meant to be a long explanation of how i came up with that idea, was just sharing it for those who wanted to try ) besides, its really a simple experiment i did and people need to understand its possible to come up with valid conclusions without having big budget research grants and expensive analytical machines and specialy labs built full of phD staffers... just use a little common sense when you design an experiment, and then it can tell you something - its really that simple. and can be appled to lots of things people argue back and forth about on the boards here, so anybody else that wants to try to experiment on some of the mycology "myths" and "vaugeries" they absolutely should (haha just be prepared for everybody to dispute your results)
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SenselessRebel
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Re: Tweaking PF jars for super fast colonization! [Re: SenselessRebel]
#5918284 - 07/31/06 11:30 AM (17 years, 5 months ago) |
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i know i in no way was giveing you credit for developing the tek and as well i didn't know you had them all side by side all in the same inc and FC. i thought you did several runs and came to the conclusion. so for that point i was incorrect. if you do have them in the same conitions then many vars can be ignored becasue they remain constant through out all the trials. what ever im done here but yea i only inputed cuz i thought you were like trying to prove, for 100% all the time, that coffee will make a benificial difference in the growth of the mushrooms. whatever im done with the cock fight later CC and good tek -SenselessRebel
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fastfred
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Re: Tweaking PF jars for super fast colonization! [Re: SenselessRebel]
#5918401 - 07/31/06 12:15 PM (17 years, 5 months ago) |
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Nice thread CC. I might have missed a few points, but I had a couple questions about your experimental design.
I'm assuming you used monoculture as you mentioned that you used an isolated sub-strain. Just hoping you would confirm this and detail that part a little.
You note faster pinning... Could this be simply because of the faster colonization or did you birth each one at 100% colonization?
Can you detail a little more on your concentration experiments? And how confident you are that 25% is the optimal concentration?
Thanks, and good work CC.
-FF
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